2010
DOI: 10.1016/j.biologicals.2009.12.004
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Application of deglycosylation and electrophoresis to the quantification of influenza viral hemagglutinins facilitating the production of 2009 pandemic influenza (H1N1) vaccines at multiple manufacturing sites in China

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Cited by 37 publications
(38 citation statements)
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“…In our previous report 14 and during this study, we found that split samples from various subtype/types (H1N1, H3N2, B) as well as H7N9 bulk samples were stable for at least 6 months in SDS-PAGE profiles (data not shown). Unlike H1N1 and H3N2 virus, the HA1 and HA2 molecules of H7N9 can be easily separated from other viral proteins in reduced conditions.…”
Section: Sds-page Analysis Of H7n9 Bulksupporting
confidence: 59%
See 1 more Smart Citation
“…In our previous report 14 and during this study, we found that split samples from various subtype/types (H1N1, H3N2, B) as well as H7N9 bulk samples were stable for at least 6 months in SDS-PAGE profiles (data not shown). Unlike H1N1 and H3N2 virus, the HA1 and HA2 molecules of H7N9 can be easily separated from other viral proteins in reduced conditions.…”
Section: Sds-page Analysis Of H7n9 Bulksupporting
confidence: 59%
“…Clearly, after deglycosylation treatment with PNGase F, the HA1 and HA2 bands became much sharper as is the case for both H5N1 and H1N1. 14,15 Although the PNGase F band is close to HA1, the 2 bands were well separated ( Fig. 1), making it easier for quantification (below).…”
Section: Sds-page Analysis Of H7n9 Bulkmentioning
confidence: 99%
“…One of the simplest techniques that have been explored as an influenza potency assay is the use of SDS‐PAGE combined with densitometry to quantify the HA content in vaccine samples 29. During the 2009 H1N1 pandemic, this assay was used to formulate vaccine for clinical trials and expedite the approval of an H1N1 vaccine in China.…”
Section: New Assay Developmentmentioning
confidence: 99%
“…Quantitative determination of influenza viral HA and NA are critical for vaccine research and development since these two surface proteins are most important viral components inducing immune responses [6][7][8][9][10][11] . Previously reported immunological methods for the detection of these proteins require strain specific antibodies.…”
Section: Discussionmentioning
confidence: 99%
“…The NA content of these reference samples was determined by SDS-PAGE analysis of deglycosylated samples in conjunction with densitometry scanning analysis, as described previously 9 , with slight modifications 10 . In brief, the deglycosylated vaccine reference samples (5 μg) were mixed with sample buffer and loaded on the gel.…”
Section: Preparation Of Influenza Vaccine Samples and Reference Standmentioning
confidence: 99%