Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying

Ramezani, Vahid; Vatanara, Alireza; Seyedabadi, Mohammad; Meibodi, Mohsen Nabi; Fanaei, Hamed

doi:10.1080/03639045.2017.1293679

Cited by 26 publications

(11 citation statements)

References 52 publications

(50 reference statements)

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“…Regarding the sd_powders (Figure 5), which are characterized by a residual water content that is higher than that of the fd_powders, an increase in thermal stability of the protein is negligible for sd_ST, while it is significant in the presence of the cyclodextrin (sd_SM and sd_SMT). These results may be explained by referring to the natural role of trehalose, which is produced by unicellular organisms to protect proteins from denaturation after exposure to stress [57], and to the ability of CDs to enhance the physical stabilization of proteins against different kinds of stress conditions, such as thermal stress [50].…”

Section: Thermal Spectroscopic and Granulometric Analysesmentioning

confidence: 99%

Effect of Methyl–β–Cyclodextrin and Trehalose on the Freeze–Drying and Spray–Drying of Sericin for Cosmetic Purposes

et al. 2021

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Sericin is a protein extracted from Bombyx mori silk cocoons. Over the last decade, this wastewater product of the textile industry has shown many interesting biological properties. This protein is widely used in the cosmetic and biomedical fields. In this study, sericin has been obtained via a High–Temperature High–Pressure degumming process, and was dried using the freeze–drying (fd) and spray–drying (sd) techniques. Proteins tend to collapse during drying, hence, sericin has been dried in the presence of two selected carrier agents: methyl–β–cyclodextrin and trehalose. The obtained powders have been analyzed using thermal investigation, microscopy (optical, SEM), and granulometric and spectroscopic analyses. Moreover, the percentage yield of the spray–drying process has been calculated. Both the agents were able to significantly improve the drying process, without altering the physico–chemical properties of the protein. In particular, the co–spray–drying of sericin with methyl–β–cyclodextrin and trehalose gave good process yields and furnished a powder with low moisture content and handling properties that are better than those of the other studied dried products. These characteristics seem to be appropriate and fruitful for the manufacturing of cosmetic raw materials.

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Section: Thermal Spectroscopic and Granulometric Analysesmentioning

confidence: 99%

Effect of Methyl–β–Cyclodextrin and Trehalose on the Freeze–Drying and Spray–Drying of Sericin for Cosmetic Purposes

et al. 2021

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“…Spray-drying is widely used in many applications of the food and pharmaceutical industries (Masters, 1991) and is an extensively used method for drying protein and improving the stability of antibodies (Ramezani et al, 2017). This process has demonstrated to maintain the quality of live-virus vaccines during storage (Li et al, 2015;Kanojia et al, 2017), which is important because the need to maintain the cold chain for these vaccines is challenging.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum

Lomónaco

Sowul

Gutiérrez

et al. 2020

Journal of Dairy Science

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Previous studies have shown the presence of bovine leukemia virus (BLV) in colostrum and milk of naturally infected cows. The relationship between virus or provirus and specific antibodies in these secretions is particular to each infected cow and will probably determine whether the consumption of colostrum or milk from these naturally infected dams provides an infective or a protective effect in recipient calves. Our recent findings suggest that this issue is a key point in BLV transmission in very young calves. Based on this, the aim of the present study was to determine the effect of the spray-drying treatment of colostrum on BLV infectivity. The treatment was done on scale-down conditions, using fresh colostrum from BLV-negative cows spiked with infective BLV. Residual infectivity was tested in susceptible lambs. Lambs inoculated with colostrum spiked with BLV-infected cells or cell-free BLV showed evidence of infection 60 d after inoculation, whereas none of the lambs inoculated with spraydried colostrum showed evidence of infection 60 d after inoculation. These results provide direct evidence that the experimental spray-drying process used in this study was effective in inactivating infectious BLV in colostrum. These findings suggest that the risk for BLV transmission could be reduced if milk and colostrum were treated by spray-drying prior to consumption in dairy facilities. The effect of spray-drying on the functional properties and stability of the antibodies present in colostrum under long-term storage should be further investigated.

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“…The solubility of proteins is directly related to adsorption at the air-liquid interface and aggregation. Techniques that expose proteins to a high liquid interfacial area (e.g., SD, SFD), often require the use of additional excipients, such as cyclodextrins (Ramezani et al, 2017), surfactants (Bhatnagar B. and Tchessalov., 2020), ethanol (Johnson, 1997), or the utilization of acidic conditions (Liu et al, 1991). These excipients may also be necessary to modulate the dissolution of the protein.…”

Section: Solubility and Dissolutionmentioning

confidence: 99%

The Development of Thin-Film Freezing and Its Application to Improve Delivery of Biologics as Dry Powder Aerosols

Hufnagel

Sahakijpijarn

Moon

et al. 2022

KONA

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While the formulation of pharmaceuticals as liquids is common practice, powders are associated with enhanced stability, avoidance of the cold chain, lower dosing requirements, and more convenient administration. These are particularly critical for proteins, as they are expensive and complicated to manufacture. Powders also have improved aerosol properties for pulmonary delivery. Conventional techniques for formulating powders include spray-drying, shelf freeze-drying, spray freeze-drying, and spray freezing into liquid, but they produce powders with poor aerosol performance and/or activity due to suboptimal powder properties. Thin-film freezing (TFF) is a new cryogenic technique that can engineer highly porous, brittle, powder matrices with excellent aerosol performance properties and stability. Herein, we describe TFF in comparison to other cryogenic techniques. Physical properties of TFF powders such as morphology, moisture sorption, stability, solubility, and dissolution, as well as aerosol properties are discussed. In addition, factors that significantly affect the physical and aerosol properties of dry powders prepared by TFF, such as solids content, drug loading, solvent system, excipient, and dry powder delivery device, are analyzed. Finally, we provide evidence supporting the applicability of using TFF to prepare dry powder formulations of protein-based pharmaceuticals, enabling their cold chain-free storage as well as efficient pulmonary delivery.

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Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying

Abstract: HPBCD is an efficient excipient for the development of inhalable protein formulations. In this regard, optimal particle property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose.

Cited by 26 publications

References 52 publications

Effect of Methyl–β–Cyclodextrin and Trehalose on the Freeze–Drying and Spray–Drying of Sericin for Cosmetic Purposes

Effect of Methyl–β–Cyclodextrin and Trehalose on the Freeze–Drying and Spray–Drying of Sericin for Cosmetic Purposes

Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum

The Development of Thin-Film Freezing and Its Application to Improve Delivery of Biologics as Dry Powder Aerosols

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