A hierarchical CuO array film was fabricated by electrochemical deposition with the aid of a colloidal monolayer as a template, followed by oxidation at high temperature. The prepared CuO film with a granular structure was arranged with ordered, hexagonal close-packed, and bowl-like pores. Combined with a gold layer sputtered on a glass substrate, this CuO array film was used as an electrochemical electrode in the amperometric detection of L-ascorbic acid. The array film exhibited a high sensitivity of 3484 μA mM -1 cm -2 , a wide linear range from 1 μM to 7 mM, and a detection limit of 0.2 μM. Excellent stability was also achieved. The results demonstrate that the hierarchical CuO bowl-like array film is a promising new platform for the construction of non-enzymatic ascorbic acid sensors.