Application of Crude Protease from Cheap and Local Raw Material as a Biopesticide for the Disruption of Pomacea Canaliculata Eggs

Abstract

Abstract-The application of crude protease of Aspergillus oryzae to suppress the hatchability of Pomacea canaliculata eggs is highlighted. The enzyme was produced through solid state fermentation (SSF) using cassava, which is cheap and abundantly available. It was found that the enzyme suppressed the hatchability of P. canaliculata eggs as much as 30% and 20% at 20.8U/ml enzyme for 3 and 9 days old eggs respectively. The ANOVA result revealed that the hatchability was significantly affected by both protease concentration as well as egg's age. The LC 50 for 3 and 9 days old eggs were 3.29 U/ml and 3.54 U/ml, respectively; while LC 95 for 3 and 9 days old eggs were 19.11 U/ml and 20.09 U/ml respectively. It is proposed that the crude protease propagated from SSF using locally available and cheap material should be considered as an alternative biopesticide to suppress the hatchability of P. canaliculata eggs.

Effects of Steam Pretreated Rice Biomass Condensate Containing Soluble Solid Inhibitors on Disruption of Pomacea Canaliculata Egg

“…One of the major concerns of rice cultivation issues in Malaysia is the spread of Pomacea canaliculate which is the only freshwater snail listed amongst the 100 worst invasive species (Lowe et al, 2000). The combination of several factors, such as feeding habits which is primarily focused on young stem and leaves (Salleh & Arbain, 2015) and the capability to eat 7-24 seedling per day (Halwart, 1994) with high fecundity and unusual characteristics of their eggs, lead to the successful establishment of their population in invaded areas (Dreon et al, 2014). There are many control strategies recommended by the Department of Agricultural (DOA) Malaysia, including cultural management, physical, chemical, and biological (Salleh et al, 2012).…”