Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines

Vilela, Julianne; Rohaim, Mohammed A.; Munir, Muhammad

doi:10.3389/fcimb.2020.581504

Cited by 15 publications

(3 citation statements)

References 101 publications

(146 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a subsequent study, they inserted two additional viral antigen expression cassettes, which included genes of infectious laryngotracheitis virus (ILTV) and avian influenza virus (AIV), into the HVT-VP2 genome [121]. Theirs and other studies demonstrated that CRISPR/Cas9 is a simple and efficient method for the generation of vector vaccines carrying more than one foreign viral gene [122,123]. In addition, the nature of the CRISPR/Cas9 system promises the possibility of editing several target sites simultaneously, which will facilitate the development of recombinant vaccines that confer protection against multiple diseases.…”

Section: Clustered Regularly Interspaced Short Palindromic Repeats (Crispr)/cas9 Systemmentioning

confidence: 99%

Methods for the Manipulation of Herpesvirus Genome and the Application to Marek’s Disease Virus Research

et al. 2021

View full text Add to dashboard Cite

Herpesviruses are a group of double-strand DNA viruses that infect a wide range of hosts, including humans and animals. In the past decades, numerous methods have been developed to manipulate herpesviruses genomes, from the introduction of random mutations to specific genome editing. The development of genome manipulation methods has largely advanced the study of viral genes function, contributing not only to the understanding of herpesvirus biology and pathogenesis, but also the generation of novel vaccines and therapies to control and treat diseases. In this review, we summarize the major methods of herpesvirus genome manipulation with emphasis in their application to Marek’s disease virus research.

show abstract

Section: Clustered Regularly Interspaced Short Palindromic Repeats (Crispr)/cas9 Systemmentioning

confidence: 99%

Methods for the Manipulation of Herpesvirus Genome and the Application to Marek’s Disease Virus Research

et al. 2021

View full text Add to dashboard Cite

show abstract

“…NHEJ is also faster than HDR, and it is also known to suppress the HDR process [ 34 ]. The combination of CRISPR/Cas9 and Cre–Lox recombination techniques provides a powerful and versatile system to excise pre-determined LoxP sites, allowing the excision of specific genetic fragments and selectable markers [ 26 , 35 ]. Through its simplicity and effectivity, CRISPR/Cas9 technology has shown to be beneficial for gene modification and provides an alternative to recombinant vaccine construction.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9 Editing of Duck Enteritis Virus Genome for the Construction of a Recombinant Vaccine Vector Expressing ompH Gene of Pasteurella multocida in Two Novel Insertion Sites

et al. 2022

View full text Add to dashboard Cite

Duck enteritis virus (DEV) and Pasteurella multocida, the causative agent of duck plague and fowl cholera, are acute contagious diseases and leading causes of morbidity and mortality in duck. The NHEJ-CRISPR/Cas9-mediated gene editing strategy, accompanied with the Cre–Lox system, have been employed in the present study to show that two new sites at UL55-LORF11 and UL44-44.5 loci in the genome of the attenuated Jansen strain of DEV can be used for the stable expression of the outer membrane protein H (ompH) gene of P. multocida that could be used as a bivalent vaccine candidate with the potential of protecting ducks simultaneously against major viral and bacterial pathogens. The two recombinant viruses, DEV-OmpH-V5-UL55-LORF11 and DEV-OmpH-V5-UL44-44.5, with the insertion of ompH-V5 gene at the UL55-LORF11 and UL44-44.5 loci respectively, showed similar growth kinetics and plaque size, compared to the wildtype virus, confirming that the insertion of the foreign gene into these did not have any detrimental effects on DEV. This is the first time the CRISPR/Cas9 system has been applied to insert a highly immunogenic gene from bacteria into the DEV genome rapidly and efficiently. This approach offers an efficient way to introduce other antigens into the DEV genome for multivalent vector.

show abstract

“…These criteria are usually needed in biotechnological and molecular biological techniques, including diagnostics and interferences (Brezgin et al, 2019;Banan, 2020). Additionally, the potential use of the CRISPR-Cas system for future vaccine development can be accomplished, as our research group has described earlier (Atasoy et al, 2019;Vilela et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Potential Use of CRISPR/Cas13 Machinery in Understanding Virus–Host Interaction

Bayoumi

Munir

2021

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Prokaryotes have evolutionarily acquired an immune system to fend off invading mobile genetic elements, including viral phages and plasmids. Through recognizing specific sequences of the invading nucleic acid, prokaryotes mediate a subsequent degradation process collectively referred to as the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-associated (Cas) (CRISPR–Cas) system. The CRISPR–Cas systems are divided into two main classes depending on the structure of the effector Cas proteins. Class I systems have effector modules consisting of multiple proteins, while class II systems have a single multidomain effector. Additionally, the CRISPR–Cas systems can also be categorized into types depending on the spacer acquisition components and their evolutionary features, namely, types I–VI. Among CRISPR/Cas systems, Cas9 is one of the most common multidomain nucleases that identify, degrade, and modulate DNA. Importantly, variants of Cas proteins have recently been found to target RNA, especially the single-effector Cas13 nucleases. The Cas13 has revolutionized our ability to study and perturb RNAs in endogenous microenvironments. The Cas13 effectors offer an excellent candidate for developing novel research tools in virological and biotechnological fields. Herein, in this review, we aim to provide a comprehensive summary of the recent advances of Cas13s for targeting viral RNA for either RNA-mediated degradation or CRISPR–Cas13-based diagnostics. Additionally, we aim to provide an overview of the proposed applications that could revolutionize our understanding of viral–host interactions using Cas13-mediated approaches.

show abstract

Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines

Cited by 15 publications

References 101 publications

Methods for the Manipulation of Herpesvirus Genome and the Application to Marek’s Disease Virus Research

Methods for the Manipulation of Herpesvirus Genome and the Application to Marek’s Disease Virus Research

CRISPR/Cas9 Editing of Duck Enteritis Virus Genome for the Construction of a Recombinant Vaccine Vector Expressing ompH Gene of Pasteurella multocida in Two Novel Insertion Sites

Potential Use of CRISPR/Cas13 Machinery in Understanding Virus–Host Interaction

Contact Info

Product

Resources

About