Application of CRISPR/Cas9 in Rapeseed for Gene Function Research and Genetic Improvement

Tian, Qingfeng; Li, Baojun; Feng, Yizhen; Zhao, Weiguo; Huang, Jinyong; Chao, Hongbo

doi:10.3390/agronomy12040824

Cited by 11 publications

(4 citation statements)

References 200 publications

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“…Compared to the extensive studies on thousands of genes in rice and Arabidopsis thaliana , the number of genes studied in B. napus is very small and the progress of research on different traits is not balanced. At the same time, the molecular mechanisms and gene regulatory networks regulating important traits are not well studied (Tian et al, 2022).…”

Section: Challeges and Perspectivesmentioning

confidence: 99%

Functional genomics of Brassica napus: Progress, challenges, and perspectives

Tan,

Han,

Dai

et al. 2024

JIPB

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Brassica napus, commonly known as rapeseed or canola, is a major oil crop contributing over 13% to the stable supply of edible vegetable oil worldwide. Identification and understanding the gene functions in the B. napus genome is crucial for genomic breeding. A group of genes controlling agronomic traits have been successfully cloned through functional genomics studies in B. napus. In this review, we present an overview of the progress made in the functional genomics of B. napus, including the availability of germplasm resources, omics databases and cloned functional genes. Based on the current progress, we also highlight the main challenges and perspectives in this field. The advances in the functional genomics of B. napus contribute to a better understanding of the genetic basis underlying the complex agronomic traits in B. napus and will expedite the breeding of high quality, high resistance and high yield in B. napus varieties.

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Section: Challeges and Perspectivesmentioning

confidence: 99%

Functional genomics of Brassica napus: Progress, challenges, and perspectives

Tan,

Han,

Dai

et al. 2024

JIPB

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“…New genomic techniques (NGTs), such as CRISPR/Cas9, have been successfully applied several times in Brassicaceae (Li et al, 2022;Tian et al, 2022;Ahmad et al, 2023). The technology has made it possible to alter multiple copies of a gene, or even alter several different genes simultaneously (multiplexing).…”

Section: For Food or Industrialmentioning

confidence: 99%

Environmental Risk Assessment Scenarios of Specific NGT Applications in <em>Brassicaceae</em> Oilseed Plants

Koller,

Cieslak,

Bauer-Panskus

2024

Preprint

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Oilseed plants of the Brassicaceae plant family are cultivated for food, feed and industrial purposes on large-scale in Europe. This review gives an overview of current market-oriented applications of new genomic techniques (NGTs) in relevant Brassicaceae oilseed crops based on a literature survey. In this respect, changes in oil quality, yield, growth and resistance to biotic and abiotic stress are goals in oilseed rape (B. napus) and camelina (C. sativa). Environmental risk assessment scenarios are developed for specific NGT applications in Brassicaceae oilseed crops with either a changed oil composition or with fitness related traits. In case of a changed oil composition an increase or decrease of polyunsaturated fatty acids (PUFA) demonstrates risks for health and survival of pollinators. In case of fitness related traits other risks were identified, i. e. an increased risk of spread and persistence of NGT plants. Furthermore, there are indications for potential disturbance of interactions with the environment, involving signalling pathways and reaction to stress conditions. It is shown that for a comprehensive risk assessment the technological potential of NGTs, the plants’ biology and the scale of releases have to be considered in combination. Therefore, the release of NGT plants into the environment for agricultural purposes will require risk assessment and monitoring of single traits as well as of combinatorial and long-term cumulative effects. In addition, risk management should develop concepts and measures to control and potentially restrict the scale of releases. This is especially relevant for NGT Brassicaceae in Europe which is a centre of diversity of this plant family.

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“…Polygenic traits will require a more intricate multiplex approach, targeting more than one locus in a single round of editing. Yield is one such trait that is complex and will likely benefit from a multiplex approach in order to achieve rapid improvement [ 120 ]. Although hybrid-CRISPR Cas enzymes have been designed for targeting multiple loci for inactivation or activation [ 121 , 122 ], the technology has yet to be applied in Brassica .…”

Section: Developing New Phenotypes Via Genome Editing Technologymentioning

confidence: 99%

“…Although hybrid-CRISPR Cas enzymes have been designed for targeting multiple loci for inactivation or activation [ 121 , 122 ], the technology has yet to be applied in Brassica . In Brassica oilseeds, yield remains poorly characterised on the molecular level, and knowledge of the genetic mechanisms controlling seed size and oil content is scarce [ 120 ]. Hence, many of the traits currently targeted for yield improvement are those that are closely related to yield, or those that are indirectly related, but highly correlated.…”

Section: Developing New Phenotypes Via Genome Editing Technologymentioning

confidence: 99%

The Global Assessment of Oilseed Brassica Crop Species Yield, Yield Stability and the Underlying Genetics

Zandberg

Fernandez

Danilevicz

et al. 2022

Plants

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The global demand for oilseeds is increasing along with the human population. The family of Brassicaceae crops are no exception, typically harvested as a valuable source of oil, rich in beneficial molecules important for human health. The global capacity for improving Brassica yield has steadily risen over the last 50 years, with the major crop Brassica napus (rapeseed, canola) production increasing to ~72 Gt in 2020. In contrast, the production of Brassica mustard crops has fluctuated, rarely improving in farming efficiency. The drastic increase in global yield of B. napus is largely due to the demand for a stable source of cooking oil. Furthermore, with the adoption of highly efficient farming techniques, yield enhancement programs, breeding programs, the integration of high-throughput phenotyping technology and establishing the underlying genetics, B. napus yields have increased by >450 fold since 1978. Yield stability has been improved with new management strategies targeting diseases and pests, as well as by understanding the complex interaction of environment, phenotype and genotype. This review assesses the global yield and yield stability of agriculturally important oilseed Brassica species and discusses how contemporary farming and genetic techniques have driven improvements.

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Application of CRISPR/Cas9 in Rapeseed for Gene Function Research and Genetic Improvement

Cited by 11 publications

References 200 publications

Functional genomics of Brassica napus: Progress, challenges, and perspectives

Functional genomics of Brassica napus: Progress, challenges, and perspectives

Environmental Risk Assessment Scenarios of Specific NGT Applications in <em>Brassicaceae</em> Oilseed Plants

The Global Assessment of Oilseed Brassica Crop Species Yield, Yield Stability and the Underlying Genetics

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