Application of core–shell technology for determination of retinol and alpha-tocopherol in breast milk

Plíšek, Jiří; Kašparová, Markéta; Solichová, Dagmar; Krčmová, Lenka Kujovská; Kučerová, Barbora; Sobótka, L.; Solich, Petr

doi:10.1016/j.talanta.2013.01.031

Cited by 14 publications

(10 citation statements)

References 37 publications

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“…For saponification, KOH or isopropanol are used . For further sample purification, LLE is additionally applied using heptane , hexane , ethyl acetate , chloroform , methyl tert ‐butyl ether , or a mixture of hexane/ethanol/acetone/toluene . Deproteinization and LLE resulted in endogenous compounds of the matrix passing into the supernatant, which may affect the separation and determination of the analytes.…”

Section: Fat‐soluble Vitaminsmentioning

confidence: 99%

“…methanol) to optimize selectivity [27]. Several methods for the determination of lipovitamins in biological fluids involve chromatographic separation using UV-Vis [31,41,43,44,48,51] or fluorescence detection (FLD) [45,47,50,56]. The LODs and LOQs of these methods are usually sufficient for the analysis of carotenoids, retinoids, and tocopherols with appropriate precision and accuracy.…”

Section: Liquid Chromatography 145 2 Fat-soluble Vitaminsmentioning

confidence: 99%

“…Reversed‐phase chromatography is a well‐suited technique for analysis of these compounds. Mobile phases are composed mainly of organic solvents, such as methanol or acetonitrile , because of the different solubilities of fat‐soluble vitamins and their reversed‐phase retention properties. Low water content is used to enhance separation of the analytes.…”

Section: Fat‐soluble Vitaminsmentioning

confidence: 99%

“…However, HPLC–FLD methods showed multiple improvements to the sensitivity in comparison with the HPLC–UV methods. For example, LOQ of α‐tocopherol obtained by UV was 0.2 μM , while the value of 21.4 nM was reached by FLD . Measurement of other analytes, such as vitamin K derivatives or vitamin D hydroxy metabolites, is limited because of their low concentrations in the blood.…”

Section: Fat‐soluble Vitaminsmentioning

confidence: 99%

“…These analytical methods require preconditioning of the samples for analysis, including the separation of fat‐soluble vitamins from complex matrices of biological samples, such as plasma or serum , adipose tissue , breast milk , and amniotic fluid . For this purpose, protein and lipid precipitation followed by an extraction step is typically applied before liquid chromatographic analysis.…”

Section: Fat‐soluble Vitaminsmentioning

confidence: 99%

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A review of chromatographic methods for the determination of water‐ and fat‐soluble vitamins in biological fluids

Karaźniewicz−Łada

Główka

2015

J of Separation Science

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Vitamins are an essential element of nutrition and thus contribute to human health. Vitamins catalyze many biochemical reactions and their lack or excess can cause health problems. Therefore, monitoring vitamin concentrations in plasma or other biological fluids may be useful in the diagnosis of various disorders as well as in the treatment process. Several chromatographic methods have been developed for the determination of these compounds in biological samples, including high-performance liquid chromatography with UV and fluorescence detection. Recently, high-performance liquid chromatography with tandem mass spectrometry methods have been widely used for the determination of vitamins in complex matrices because of their high sensitivity and selectivity. This method requires preconditioning of samples for analysis, including protein precipitation and/or various extraction techniques. The choice of method may depend on the desired cost, convenience, turnaround time, specificity, and accuracy of the information to be obtained. This article reviews the recently reported chromatographic methods used for determination of vitamins in biological fluids. Relevant papers published mostly during the last 5 years were identified by an extensive PubMed search using appropriate keywords. Particular attention was given to the preparation steps and extraction techniques. This report may be helpful in the selection of procedures that are appropriate for certain types of biological materials and analytes.

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Section: Fat‐soluble Vitaminsmentioning

confidence: 99%

Section: Liquid Chromatography 145 2 Fat-soluble Vitaminsmentioning

confidence: 99%

Section: Fat‐soluble Vitaminsmentioning

confidence: 99%

Section: Fat‐soluble Vitaminsmentioning

confidence: 99%

Section: Fat‐soluble Vitaminsmentioning

confidence: 99%

See 3 more Smart Citations

A review of chromatographic methods for the determination of water‐ and fat‐soluble vitamins in biological fluids

Karaźniewicz−Łada

Główka

2015

J of Separation Science

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Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and α-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography†

et al. 2013

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The reduction of analysis time, cost, and improvement of separation efficiency are the main requirements in the development of high-throughput assay methods in bioanalysis. It can be achieved either by ultra-high-performance liquid chromatography (UHPLC) using stationary phases with small particles (<2 μm) at high back pressures or by using opposite direction--monolithic stationary phases with low back pressures. The application of new types of monolithic stationary phases for UHPLC is a novel idea combining these two different paths. The aim of this work was to test the recently introduced second-generation of monolithic column Chromolith® HighResolution for UHPLC analysis of liposoluble vitamins in comparison with core-shell and fully porous sub-2 μm columns with different particle sizes, column lengths, and shapes. The separation efficiency, peak shape, resolution, time of analysis, consumption of mobile phase, and lifetime of columns were calculated and compared. The main purpose of the study was to find a new, not only economical option of separation of liposoluble vitamins for routine practice.

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Recent advances in the determination of tocopherols in biological fluids: from sample pretreatment and liquid chromatography to clinical studies

et al. 2016

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Vitamin E comprises eight related compounds: α-, β-, γ-, δ-tocopherols and α-, β-, γ-, δ-tocotrienols. In the past, α-tocopherol has been the isomer that was studied most, and its anti-inflammatory and anti-proliferative effects have been described. Therefore, many prevention trials have investigated the effect of α-tocopherol on human health. Current research studies have also defined the important roles of other tocopherols, such as anti-inflammatory, anti-proliferative and cancer preventative effects. Knowledge of the individual tocopherols could help to understand their roles in various metabolic pathways. This review summarizes the recent trends in sample pretreatment, liquid chromatography and selected applications of the determination of tocopherols in various biological materials. The relationship between tocopherol isomers and serious diseases is also described. Graphical Abstract Article structure.

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Application of core–shell technology for determination of retinol and alpha-tocopherol in breast milk

Cited by 14 publications

References 37 publications

A review of chromatographic methods for the determination of water‐ and fat‐soluble vitamins in biological fluids

A review of chromatographic methods for the determination of water‐ and fat‐soluble vitamins in biological fluids

Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and α-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography†

Recent advances in the determination of tocopherols in biological fluids: from sample pretreatment and liquid chromatography to clinical studies

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