Application of computer‐aided sperm analysis (CASA) for detecting sperm‐immobilizing antibody

Wakimoto, Yu; Fukui, Atsushi; Kojima, Teruhito; Hasegawa, Akiko; Shigeta, Minoru; Shibahara, Hiroaki

doi:10.1111/aji.12814

Cited by 16 publications

(18 citation statements)

References 31 publications

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“…Recently, our group applied the computer‐aided sperm analysis (CASA) for detecting sperm‐immobilizing antibodies as these tests have been carried out by counting the number of motile sperm under a microscope, suggesting that the results may vary because of subjective judgement of the operator, as seen with routine semen examination 20 . We developed a novel method using CASA, and the results were compared with those obtained by the traditional method.…”

Section: Detection Of Anti‐sperm Antibodiesmentioning

confidence: 99%

Anti‐sperm antibodies and reproductive failures

Shibahara

Wakimoto

Fukui

et al. 2020

American J Rep Immunol

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Reproductive failures include infertility and recurrent pregnancy loss (RPL). Although the relative importance of immunological factors in human reproduction remains unclear, there may be immune‐mediated reproductive failures in a portion of unexplained infertility and RPL. As a cause of immunological factor, anti‐sperm antibodies (ASA) are produced both in men and women. There have been reported several antigens in the surface of sperm that are especially foreign to women. The presence of ASA, especially sperm‐immobilizing antibodies, in the sera of infertile women has been shown to inhibit sperm migration in the female genital tract. Therefore, the effectiveness of the treatments for infertile women with sperm‐immobilizing antibodies by timed intercourse or intra‐uterine insemination is limited. Such antibodies can also exert inhibitory effects on various stages of sperm‐egg interaction and subsequent embryo development in vitro. It is suggested that ASA testing for infertile women should be performed before proceeding IVF. The manipulation of gametes and embryos from patients having sperm‐immobilizing antibodies should be carefully carried out especially to avoid contaminating patient's serum and follicular fluid in the culture medium in order to overcome the immunological causes of female infertility by ASA, and satisfactory results under suitable conditions for gametes and embryos have been obtained. The relationship between ASA and RPL was controversially reported. Increased miscarriage rates in women with ASA were demonstrated by some authors. In contrast, lack of association between ASA and RPL was reported. In this manuscript, we are focusing the roles of ASA in women with reproductive failures.

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Section: Detection Of Anti‐sperm Antibodiesmentioning

confidence: 99%

Anti‐sperm antibodies and reproductive failures

Shibahara

Wakimoto

Fukui

et al. 2020

American J Rep Immunol

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“…To manage this, we recently outlined a new method using CASA to make SIT evaluation more convenient and objective as compared to the traditional method 11 . We found the assay results for detecting the SI antibodies and estimating the quantitative titer of SI antibodies to be significantly correlated between the traditional method of manually counting sperm by eye estimation by a highly skilled embryologist and the new method of counting sperm through the chamber slides using CASA 11 . Moreover, this study provided evidence that a highly skilled embryologist is not necessarily required in order to perform this test.…”

Section: Discussionmentioning

confidence: 99%

Sperm immobilization test and quantitative sperm immobilization test using frozen‐thawed sperm preparation applied with computer‐aided sperm analysis

Wakimoto

Fukui

Kojima

et al. 2021

Reprod Medicine & Biology

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Purpose In a previous study, a new method was described using the sperm immobilization test (SIT) with computer‐aided sperm analysis (CASA). However, obtaining high‐quality sperm as needed was a known issue. Here, we compared the results of using frozen‐thawed sperm and fresh sperm for the SIT using the CASA method. Methods For the frozen‐thawed preparation, 500 μL of condensed semen and 500 μL of Sperm Freeze were mixed in a cryovial and cryopreserved in liquid nitrogen. Density gradient centrifugation was used for the collection of motile sperm in both the fresh and frozen‐thawed sperm preparations. A total of 50 serum samples were prepared for both the fresh and frozen‐thawed sperm with each sample tested containing 10 μL of serum, 1 μL of either fresh or frozen motile sperm suspension, and 2 μL of complement. Sperm motilities were measured using CASA after a 1‐hour incubation period for both fresh and frozen‐thawed sperm. Results Both fresh and frozen‐thawed sperm reacted similarly when exposed to serum containing sperm‐immobilizing antibodies asserting the use of frozen‐thawed sperm for the diagnosis of immunological infertility. Conclusion These results suggest the possibility of using cryopreserved sperm for the SIT when fresh sperm is unavailable.

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“…Over the decades, the CASA system has developed protocols to efficiently analyze the kinematic parameters of sperm in animals [ 98 , 99 ]. However, its use is not just limited to kinematic parameters, it can also analyze morphology, viability, DNA fragmentation, and acrosome reaction [ 100 ], which are considered vital for fertility determination.…”

Section: Sperm Motilitymentioning

confidence: 99%

Semen evaluation: methodological advancements in sperm quality-specific fertility assessment

et al. 2021

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Assessment of male fertility is based on the evaluation of sperm. Semen evaluation measures various sperm quality parameters as fertility indicators. However, semen evaluation has limitations, and it requires the advancement and application of strict quality control methods to interpret the results. This article reviews the recent advances in evaluating various sperm-specific quality characteristics and methodologies, with the help of different assays to assess sperm-fertility status. Sperm evaluation methods that include conventional microscopic methods, computer-assisted sperm analyzers (CASA), and flow cytometric analysis, provide precise information related to sperm morphology and function. Moreover, profiling fertility-related biomarkers in sperm or seminal plasma can be helpful in predicting fertility. Identification of different sperm proteins and diagnosis of DNA damage has positively contributed to the existing pool of knowledge about sperm physiology and molecular anomalies associated with different infertility issues in males. Advances in methods and sperm-specific evaluation has subsequently resulted in a better understanding of sperm biology that has improved the diagnosis and clinical management of male factor infertility. Accurate sperm evaluation is of paramount importance in the application of artificial insemination and assisted reproductive technology. However, no single test can precisely determine fertility; the selection of an appropriate test or a set of tests and parameters is required to accurately determine the fertility of specific animal species. Therefore, a need to further calibrate the CASA and advance the gene expression tests is recommended for faster and field-level applications.

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Application of computer‐aided sperm analysis (CASA) for detecting sperm‐immobilizing antibody

Abstract: Using the novel method, manually counting the number of motile spermatozoa becomes unnecessary. The novel method presented here will increase the objectivity and convenience of using the SIT as a clinical indicator.

Cited by 16 publications

References 31 publications

Anti‐sperm antibodies and reproductive failures

Anti‐sperm antibodies and reproductive failures

Sperm immobilization test and quantitative sperm immobilization test using frozen‐thawed sperm preparation applied with computer‐aided sperm analysis

Semen evaluation: methodological advancements in sperm quality-specific fertility assessment

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