Application of comparative genomics in chicken gene mapping

Abstract

The NDRG4 gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may be involved in the regulation of mitogenic signalling in vascular smooth muscle cells. To map this gene in the chicken chromosome, a 6000 rads chicken-hamster radiation hybrid panel (ChickRh6) was used. primers were designed according to the published human sequence for amplification of the chicken NDRG4 gene by comparative genomics. We compared the corresponding human mRNa sequence with the predicted coding sequence of the chicken A07089;