Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes

Abstract

This study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/ mL) at 37 °C for 4 h followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further information on toxicology should be obtained in future studies.Uniterms: Genotoxicity. White bean flour/genotoxic effects. Comet assay/toxicological analysis of food. Food/toxicological analysis.Este estudo foi realizado para avaliar o potencial de indução de efeitos genotóxicos da farinha de feijão branco utilizando o teste do Cometa. O ensaio foi realizado com linfócitos humanos presentes no sangue imediatamente após a coleta, por incubação com farinha de feijão branco em três concentrações (3,92, 9,52 e 18,18 mg/mL) a 37 °C por 4 h, seguida de preparação das lâminas. As amostras foram consideradas positivas (acima de 20% de dano), quando os danos observados no DNA celular foram maiores do que o controle negativo. Verificou-se que as doses testadas não mostraram potencial genotóxico. No entanto, seria prematuro fazer recomendações sobre o padrão de riscos para a saúde humana resultantes de danos ao DNA já que exposição das células ao extrato foi restrito ao período de quatro horas e não durante um ciclo celular completo. Além disso, outras informações sobre a toxicologia devem ser obtidas no futuro.Unitermos: Genotoxicidade. Farinha de feijão branco/efeitos genotóxicos. Teste do Cometa/análise toxicológica de alimentos. Alimentos/análise toxicológica.

Phaseolin, a Protein from the Seed of Phaseolus vulgaris, Has Antioxidant, Antigenotoxic, and Chemopreventive Properties

“…Various reports using beans have yielded congruent results. Lopes et al [ 54 ] examined the genotoxic potential of white bean flour by means of the comet assay and found no effect, a result that was related to the presence of polyphenolic compounds, including tannins, terpenes, and lectins. In addition, Azevedo et al [ 13 ] found no DNA damage with the comet and the micronucleus tests when cooked and dehydrated P. vulgaris (1–20%) were given to mice for 15 days; in contrast, they observed genoprotection against the damage induced by cyclophosphamide.…”

Cytotoxicity, genotoxicity, and mutagenicity potential of ethanolic extract of Heracleum persicum Desf. ex Fischer

“…We used normal human lymphocyte, which commonly regarded as biological model for this aim (Natalia Bailon‐Moscoso et al, ). It is stated that the basic mechanisms of DNA damage are alike in various tissues, therefore recommending that the level of damage in lymphocytes may reflect those happening in other tissues (Silva Pereira et al, ). In this study, we used comet assay to evaluate genotoxicity of EEHP.…”

Toxicity Effect of Ultraviolet (UV) Radiations on the Human Germ Cell and the Protective Role of Vitamin E

“…Viable cells transferred into normalmelting-point agarose, detergents and alkaline salts were used the cell membrane and placed horizontal electrophoresis tank. Damaged DNA of cell run to anode of electrophoresis with single and/or double strand breaks under alkaline conditions, cells transform into head and tail of comet [14,15]. If DNA single or double-strand breaks are not repaired, mutated cells, micronuclei, cell anomaly, and mutations can be formed in the cell [16] .…”