Abstract:Routine test of cerebrospinal fluid (CSF), such as glucose concentrations, chloride ion, protein and leukocyte, as well as color, turbidity and clot, were important indicators for intracranial infection. However, there were no models to predict the intracranial infection with these parameters. We collected data of 221 cases with CSF positive-culture and 50 cases with CSF negative culture from January 1, 2016 to December 31, 2018 in the First Affiliated Hospital of Nanchang University, China. SPSS17.0 software … Show more
“…It’s been reported that postoperative infection in patients with cerebrovascular diseases, craniocerebral trauma and craniocerebral tumors is relatively high, and its possible causes are closely related to the types of surgery, surgical methods and the characteristics of surgery in patients with this disease [ 15 , 16 ]. Patients with cerebrovascular diseases and craniocerebral injuries mainly undergo emergency surgery in clinical settings, and there are often insufficient pre-operative preparations and incomplete debridement during surgery [ 17 ]. Therefore, patients have a relatively high risk of intracranial infection.…”
Background
Intracranial infection after puncture of cerebral hematoma in patients with intracerebral hemorrhage is very common in the department of neurosurgery, yet the relevant risks remain unknown. We attempted to analyze the risk factors of intracranial infection after puncture of cerebral hematoma, to provide insights into the management of patients with intracerebral hemorrhage after puncture of cerebral hematoma.
Methods
Patients with intracerebral hemorrhage after puncture of cerebral hematoma treated in our hospital from January 2017 to January 2020 were selected, the related characteristics of intracranial infection and no infection patients were compared. Logistic regression analyses were conducted to analyze the risk factors for intracranial infection after puncture of cerebral hematoma.
Results
A total of 925 patients with puncture of cerebral hematoma were included. The incidence of postoperative intracranial infection was 7.03%. There were significant statistical differences between the infected group and the no infection group in the American Association of Anesthesiologists (ASA) grade, length of hospital stay, consecutive operation, duration of surgery, extra-ventricular drainage (EVD) use (all p < 0.05). There was statistically significant difference in the duration of EVD between the infection and no infection groups (p = 0.002), and there was no significant difference in the frequency of EVD insertion between the two groups (p = 0.094). The length of hospital stay≥10 days (OR1.832, 1.062–3.158), consecutive operation (OR2.158, 1.358–3.430), duration of surgery≥4 h (OR1.581, 1.031–2.425), EVD use (OR1.694, 1.074–2.670), and duration of EVD ≥ 7 days (OR2.699, 1.689–4.311) were the risk factors of intracranial infection in patients with intracerebral hemorrhage after puncture of cerebral hematoma (all p < 0.05).
Conclusion
Clinical medical workers should take corresponding preventive measures against the different risk factors for prevention of intracranial infection in patient with puncture of cerebral hematoma.
“…It’s been reported that postoperative infection in patients with cerebrovascular diseases, craniocerebral trauma and craniocerebral tumors is relatively high, and its possible causes are closely related to the types of surgery, surgical methods and the characteristics of surgery in patients with this disease [ 15 , 16 ]. Patients with cerebrovascular diseases and craniocerebral injuries mainly undergo emergency surgery in clinical settings, and there are often insufficient pre-operative preparations and incomplete debridement during surgery [ 17 ]. Therefore, patients have a relatively high risk of intracranial infection.…”
Background
Intracranial infection after puncture of cerebral hematoma in patients with intracerebral hemorrhage is very common in the department of neurosurgery, yet the relevant risks remain unknown. We attempted to analyze the risk factors of intracranial infection after puncture of cerebral hematoma, to provide insights into the management of patients with intracerebral hemorrhage after puncture of cerebral hematoma.
Methods
Patients with intracerebral hemorrhage after puncture of cerebral hematoma treated in our hospital from January 2017 to January 2020 were selected, the related characteristics of intracranial infection and no infection patients were compared. Logistic regression analyses were conducted to analyze the risk factors for intracranial infection after puncture of cerebral hematoma.
Results
A total of 925 patients with puncture of cerebral hematoma were included. The incidence of postoperative intracranial infection was 7.03%. There were significant statistical differences between the infected group and the no infection group in the American Association of Anesthesiologists (ASA) grade, length of hospital stay, consecutive operation, duration of surgery, extra-ventricular drainage (EVD) use (all p < 0.05). There was statistically significant difference in the duration of EVD between the infection and no infection groups (p = 0.002), and there was no significant difference in the frequency of EVD insertion between the two groups (p = 0.094). The length of hospital stay≥10 days (OR1.832, 1.062–3.158), consecutive operation (OR2.158, 1.358–3.430), duration of surgery≥4 h (OR1.581, 1.031–2.425), EVD use (OR1.694, 1.074–2.670), and duration of EVD ≥ 7 days (OR2.699, 1.689–4.311) were the risk factors of intracranial infection in patients with intracerebral hemorrhage after puncture of cerebral hematoma (all p < 0.05).
Conclusion
Clinical medical workers should take corresponding preventive measures against the different risk factors for prevention of intracranial infection in patient with puncture of cerebral hematoma.
“…Analyzing CSF aids the diagnosis of CNS diseases . The routine clinical analysis of CSF relies on cell culture, latex agglutination tests, and immunologic and biochemical analyses. , Despite being well established, these methods have intrinsic limitations, such as complicated sample pretreatment, large sample consumption, long response time, etc. In our method, 1 μL of CSF was directly spotted on the self-assembled AuNP arrays for LDI-MS analysis.…”
Section: Resultsmentioning
confidence: 99%
“…Indeed, the reduction of CSF glucose levels is a clinical sign of bacterial CNS infection. , For absolute quantification of glucose in CSF, cellobiose was introduced as an internal standard (IS) due to its similar ionization efficiency compared to glucose. As shown in Figure f, peaks at m / z 203 and 365, corresponding to [glucose + Na] + and [cellobiose + Na] + , could be clearly detected by the ISANAS-assisted LDI-MS from 1 μL of artificial CSF containing different concentrations of glucose and 1 mM cellobiose.…”
Precise
and rapid monitoring of metabolites in biofluids is a desirable
but unmet goal for disease diagnosis and management. Matrix-assisted
laser desorption/ionization mass spectrometry (MALDI-MS) exhibits
advantages in metabolite analysis. However, the low accuracy in quantification
of the technique limits its transformation to clinical usage. We report
herein the use of Au nanoparticle arrays self-assembled at liquid–liquid
interfaces for mass spectrometry (MS)-based quantitative biofluids
metabolic profiling. The two-dimensional arrays feature uniformly
and closely packed Au nanoparticles with 3 nm interparticle gaps.
The experimental study and theoretical simulation show that the arrays
exhibit high photothermal conversion and heat confinement effects,
which enhance the laser desorption/ionization efficacy. With the nanoscale
roughness, the AuNP arrays as laser desorption/ionization substrates
can interrupt the coffee-ring effect during droplet evaporation. Therefore,
high reproducibility (RSD <5%) is obtained, enabling accurate quantitative
analysis of diverse metabolites from 1 μL of biofluids in seconds.
By quantifying glucose in the cerebrospinal fluid (CSF), it allows
us to identify patients with brain infection and rapidly evaluate
the clinical therapy response. Consequently, the method shows potential
in advanced metabolite analysis and biomedical diagnostics.
“…Elevated protein levels and red blood cell counts in CSF were also detected in patients with viral encephalitis (14)(15)(16). Studies have shown that lower chloride levels (<120 mEq/L) might be seen in tuberculous, cryptococcal and bacterial meningitis (17,18).…”
Objective: Altered mental status (AMS) is challenging diagnosis. It was aimed to evaluate the underlying causes, reveal laboratory, microbiological and imaging findings, and determine the infectious process in older patients who presented with AMS.
Materials and Methods:This retrospective study was conducted at a training and research hospital. Sixty-five year and older patients who presented with AMS and underwent lumbar puncture, were included.Results: Among 98 older patients with AMS, the median age was 75.0 years (interquartile range: 69.0-75.0) and 58.2% of patients were female. Of the patients, 26.5% meningitis/encephalitis, 33.6% other infection sources, and 39.8% other disorders were found, respectively. Cerebrospinal fluid (CSF) white blood cell and protein levels were found higher; CSF chloride levels were detected lower in meningitis/encephalitis group. In brain, magnetic resonance imaging (p<0.001) and electroencephalogram (p=0.009) were found more pathologies suggesting infection in meningitis/ encephalitis patients, while brain computed tomography revealed no differences between meningitis/encephalitis and other diagnoses group. The need for intensive care was higher in the other disorder group (p=0.02) while admission to service was higher in the meningitis/encephalitis group (p=0.03).
Conclusion:Clinical characteristics failed to differentiate between meningitis/encephalitis and other diagnoses in older patients with AMS, and CSF analysis, cranial imaging methods were required for the final diagnosis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.