Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species

Kusunoki, Shinji; Ezaki, Takayuki; Tamesada, Makoto; Hatanaka, Y.; Asano, Kosuke; Hashimoto, Yasuhiro; Yabuuchi, Eiko

doi:10.1128/jcm.29.8.1596-1603.1991

Cited by 127 publications

(51 citation statements)

References 19 publications

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“…Thus, the Ad Hoc Committee of International Systematic Bacteriology had released recommendation that the DNA sequence for at least five housekeeping genes be used for taxonomy as alternative to using chromosomal DNA-DNA similarity (28). rpoB, gyrB, ITS, and groEL are candidate because they have larger sequence variation than the 16S rDNA sequence (17). The rpoB sequence of members of the Enterobacteriaceae family has been determined in detail and has proven to be promising for differentiating closely related species within this family Enterobacteriaceae (20).…”

Section: Discussionmentioning

confidence: 99%

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Quantitative Microarray‐Based DNA‐DNA Hybridization Assay for Measuring Genetic Distances among Bacterial Species and Its Application to the Identification of Family Enterobacteriaceae

Amano¹,

Ohkusu

Kusaba

et al. 2005

Microbiology and Immunology

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The sequences of nearly 6,000 taxonomically described species and bacteria have been reclassified according to their 16S ribosomal DNA (rDNA) sequences. As a result, ribosomal RNA sequencing has become a powerful method for determining the phylogenetic position of unidentified strains. All together, the sequences of more than 60,000 16S rDNAs covering more than 97% of the sequences of officially described species has been accumulated. This data has revealed that many established species have nearly identical rRNA sequences. Thus, given sequencing errors and variations among the five to eight copies of the 16S rDNA operon on a chromosome from a single species, it is practically impossible to identify an isolated strain by sequencing if the strain less than 2% different than a closely related established species. The Ad Hoc Committee of the International Systematic Bacteriology recommended that, if the 16S rDNA sequence variation between a new strain and an established species was less than 3%, chromosomal DNA-DNA hybridization data is needed to describe the new species (18). Since the last century, members of the family Enterobacteriaceae have been classified according to their biochemical traits (3). For this reason, genetically identical organisms, such as four species of genus Shigella and Escherichia coli, had been misclassified as different species (26,27). Also, early in the 20th century, more than 2,000 Salmonella serovars had been classified as independent species, but they are now known to be a single species (33).Chromosomal DNA-DNA hybridization can solve the problems of phenotypic identification and 16S rDNA sequencing. A bacterial species is genetically defined as a group of strains that shares more than 70% Abstract: Quantitative DNA-DNA hybridization to measure the genetic distances among bacterial species is indispensable for taxonomical determination. In the current studies, we developed a method to determine bacterial DNA relatedness on a glass microarray. Reference DNAs representing a total 93 species of Enterobacteriaceae were arrayed on a glass microplate, and signal intensities were measured after 2 hr of hybridization with Cy3-labeled bacterial DNAs. All immobilized DNAs from members of the family Enterobacteriaceae were identified by this method except for DNAs from Yersinia pseudotuberculosis and Y. pestis. These results suggest that quantitative microarray hybridization could be an alternative to conventional DNA-DNA hybridization for measuring chromosome relatedness among bacterial species.

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Section: Discussionmentioning

confidence: 99%

“…Type strain hybridized to reference DNAs -------------GTC 109 GTC 118 tion (7,8,17). In this technique, DNA is fixed to the surface of the microtiter wells by physical adsorption.…”

Section: Labeled Strains Strongly Cross-array Positionmentioning

confidence: 99%

Quantitative Microarray‐Based DNA‐DNA Hybridization Assay for Measuring Genetic Distances among Bacterial Species and Its Application to the Identification of Family Enterobacteriaceae

Amano¹,

Ohkusu

Kusaba

et al. 2005

Microbiology and Immunology

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“…The clinical features of pulmonary MAC disease presenting as a solitary nodule have been described in only one report. 6 Recently, because of improved techniques for isolating and identifying non-tuberculous mycobacteria, [7][8][9] an increase in the frequency of isolation of non-tuberculous mycobacteria has been observed. The present study aimed to evaluate the clinical findings and management of patients in Japan who had a solitary pulmonary nodule due to MAC.…”

Section: Introductionmentioning

confidence: 99%

Four cases of pulmonary Mycobacterium avium intracellulare complex presenting as a solitary pulmonary nodule and a review of other cases in Japan

et al. 2006

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“…The present ¢ndings thus indicate the possibility of testing for 18-kDa HSP expression at 45³C by a given MAC strain to di¡erentiate MAV and MIN, for clinical diagnosis of MAC infections. This test can be easily performed with a low cost, in contrast to diagnostic kits based on a DNA probe test (AccuProbe) [3] or quantitative DNA^DNA hybridization (DDH mycobacteria) [15] that are expensive and, moreover, require special apparatus.…”

Section: Resultsmentioning

confidence: 99%

Expression profiles of low molecular mass heat shock proteins by Mycobacterium avium and Mycobacterium intracellulare

Tomioka

2001

FEMS Microbiology Letters

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Closely related non-tuberculous mycobacterial species, Mycobacterium avium and Mycobacterium intracellulare, were compared for the profiles of their production of low molecular mass heat shock proteins at 45³C, by performing polyacrylamide gel electrophoresis analysis of bacterial cell lysate proteins. All of the M. intracellulare but not M. avium strains potently increased the production of the 18-kDa heat shock protein, when cultured at 45³C. Half of the M. intracellulare strains with high sensitivity to 45³C produced not only the 18-kDa heat shock protein but also the 16-kDa heat shock protein at 45³C. These findings indicate that M. avium and M. intracellulare differentially respond to 45³C heat shock in terms of the production of low molecular mass heat shock proteins. ß

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Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species

Cited by 127 publications

References 19 publications

Quantitative Microarray‐Based DNA‐DNA Hybridization Assay for Measuring Genetic Distances among Bacterial Species and Its Application to the Identification of Family Enterobacteriaceae

Quantitative Microarray‐Based DNA‐DNA Hybridization Assay for Measuring Genetic Distances among Bacterial Species and Its Application to the Identification of Family Enterobacteriaceae

Four cases of pulmonary Mycobacterium avium intracellulare complex presenting as a solitary pulmonary nodule and a review of other cases in Japan

Expression profiles of low molecular mass heat shock proteins by Mycobacterium avium and Mycobacterium intracellulare

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