Application of chitosan with different molecular weights in cartilage tissue engineering

Zhang, Runjie; Chang, Shwu Jen; Jing, Yanzhen; Wang, Liyuan; Liu, Jen‐Tsai

doi:10.1016/j.carbpol.2023.120890

Cited by 16 publications

(4 citation statements)

References 159 publications

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“…In addition to the differences in molecular weight between the different types of CH tested (low vs. medium molecular weight), their deacetylation degree (DD) differs slightly, with CH-LW having a slightly higher DD (a higher percentage of amino groups in the molecular chain) than CH-MW. Some authors [ 56 ] have reported that molecular weight influences the surface properties, as well as the content of functional groups and the structure of the molecular chains, in the case of CH with similar DD. Both structural parameters (DD and molecular weight) affect the interaction of CH with other compounds, which could justify the different behavior observed in Figure 6 a,b.…”

Section: Resultsmentioning

confidence: 99%

The Potential of Chitosan-Based Composites for Adsorption of Diarrheic Shellfish Toxins

Leal,

Amado,

Lourenço

et al. 2024

Toxins

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Okadaic acid (OA) is one of the most potent marine biotoxins, causing diarrheal shellfish poisoning (DSP). The proliferation of microalgae that produce OA and its analogues is frequent, threatening human health and socioeconomic development. Several methods have been tested to remove this biotoxin from aquatic systems, yet none has proven enough efficacy to solve the problem. In this work, we synthesized and characterized low-cost composites and tested their efficacy for OA adsorption in saltwater. For the synthesis of the composites, the following starting materials were considered: chitosan of low and medium molecular weight (CH-LW and CH-MW, respectively), activated carbon (AC), and montmorillonite (MMT). Characterization by vibrational spectroscopy (FTIR), X-ray diffraction (XRD), and microscopy revealed differences in the mode of interaction of CH-LW and CH-MW with AC and MMT, suggesting that the interaction of CH-MW with MMT has mainly occurred on the surface of the clay particles and no sufficient intercalation of CH-MW into the MMT interlayers took place. Among the composites tested (CH-LW/AC, CH-MW/AC, CH-MW/AC/MMT, and CH-MW/MMT), CH-MW/MMT was the one that revealed lower OA adsorption efficiency, given the findings evidenced by the structural characterization. On the contrary, the CH-MW/AC composite revealed the highest average percentage of OA adsorption (53 ± 11%). Although preliminary, the results obtained in this work open up good perspectives for the use of this type of composite material as an adsorbent in the removal of OA from marine environments.

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Section: Resultsmentioning

confidence: 99%

The Potential of Chitosan-Based Composites for Adsorption of Diarrheic Shellfish Toxins

Leal,

Amado,

Lourenço

et al. 2024

Toxins

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“…Furthermore, increasing the molecular weight of chitosan led to an increase in the scaffold’s elastic modulus. This effect can be attributed to the entanglement of polymer chains [ 68 ].…”

Section: Resultsmentioning

confidence: 99%

Chitosan Scaffolds as Microcarriers for Dynamic Culture of Human Neural Stem Cells

et al. 2023

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Human neural stem cells (hNSCs) possess remarkable potential for regenerative medicine in the treatment of presently incurable diseases. However, a key challenge lies in producing sufficient quantities of hNSCs, which is necessary for effective treatment. Dynamic culture systems are recognized as a powerful approach to producing large quantities of hNSCs required, where microcarriers play a critical role in supporting cell expansion. Nevertheless, the currently available microcarriers have limitations, including a lack of appropriate surface chemistry to promote cell adhesion, inadequate mechanical properties to protect cells from dynamic forces, and poor suitability for mass production. Here, we present the development of three-dimensional (3D) chitosan scaffolds as microcarriers for hNSC expansion under defined conditions in bioreactors. We demonstrate that chitosan scaffolds with a concentration of 4 wt% (4CS scaffolds) exhibit desirable microstructural characteristics and mechanical properties suited for hNSC expansion. Furthermore, they could also withstand degradation in dynamic conditions. The 4CS scaffold condition yields optimal metabolic activity, cell adhesion, and protein expression, enabling sustained hNSC expansion for up to three weeks in a dynamic culture. Our study introduces an effective microcarrier approach for prolonged expansion of hNSCs, which has the potential for mass production in a three-dimensional setting.

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“…Chitosan (Figure S11), derived from the deacetylation of chitin and primarily composed of glucosamine units, serves as the primary structural component found in crustacean exoskeletons and is an assembly constituent of glycosaminoglycan in ECM [12,22]. With features such as biodegradability, biocompatibility, non-toxicity, antibacterial properties, anticancer effects, lipid-lowering capabilities, and immune enhancement, it finds extensive applications in fields like drug delivery [120,121], medical absorbable materials [122], tissue engineering [123], and pharmaceutical development [124]. Its macromolecular chain contains an abundant amount of amino groups, hydroxyl groups, and ether bonds, allowing for modification reactions such as acylation, esterification, carboxylation, etherification, oxidation, and Schiff base formation [125,126].…”

Section: Chitosanmentioning

confidence: 99%

Applications of Light-Based 3D Bioprinting and Photoactive Biomaterials for Tissue Engineering

Zhang,

et al. 2023

Materials

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The emergence of additive manufacturing, commonly referred to as 3D printing, has led to a revolution in the field of biofabrication. Numerous types of 3D bioprinting, including extrusion bioprinting, inkjet bioprinting, and lithography-based bioprinting, have been developed and have played pivotal roles in driving a multitude of pioneering breakthroughs in the fields of tissue engineering and regenerative medicine. Among all the 3D bioprinting methods, light-based bioprinting utilizes light to crosslink or solidify photoreactive biomaterials, offering unprecedented spatiotemporal control over biomaterials and enabling the creation of 3D structures with extremely high resolution and precision. However, the lack of suitable photoactive biomaterials has hindered the application of light-based bioprinting in tissue engineering. The development of photoactive biomaterials has only recently been expanded. Therefore, this review summarizes the latest advancements in light-based 3D bioprinting technologies, including the development of light-based bioprinting techniques, photo-initiators (PIs), and photoactive biomaterials and their corresponding applications. Moreover, the challenges facing bioprinting are discussed, and future development directions are proposed.

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Application of chitosan with different molecular weights in cartilage tissue engineering

Cited by 16 publications

References 159 publications

The Potential of Chitosan-Based Composites for Adsorption of Diarrheic Shellfish Toxins

The Potential of Chitosan-Based Composites for Adsorption of Diarrheic Shellfish Toxins

Chitosan Scaffolds as Microcarriers for Dynamic Culture of Human Neural Stem Cells

Applications of Light-Based 3D Bioprinting and Photoactive Biomaterials for Tissue Engineering

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