Application of chitosan microparticles for mitigation of
            <i>Salmonella</i>
            in agricultural water

Fan, Ying; Ginn, Amber; Ma, Zhengxin; Kang, Myeong Jin; Jeong, Kwang Cheol; Wright, Anita C.

doi:10.1111/jam.13566

Cited by 11 publications

(10 citation statements)

References 41 publications

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“…The most notorious and common pathogenic Escherichia coli ( E. coli ) O157:H7, Salmonella Typhimurium and Salmonella Pullorum strains are primary enteric pathogens that not only inhibit human and animal health but also cause food poisoning and food-related epidemics around the world (Sable et al, 2000; Ma et al, 2016; Fan et al, 2017). Especially, fresh produce, such as milk, meat, and egg, is increasingly related to the incidence of foodborne illness, and outbreaks associated with fresh produce consumption by human or animals have risen substantially recently (Centers for Disease Control and Prevention [CDC], 2010, 2013).…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Antimicrobial Activity Evaluation of the Recombinant Microcin J25 Against the Foodborne Pathogens Salmonella and E. coli O157:H7 by Using a Matrix of Conditions

Zeng

et al. 2019

Front. Microbiol.

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Natural microcin J25 (MccJ25) represent promising alternatives to traditional antibiotics for the treatment of drug-resistant infections. However, little is known about the antibacterial activity of recombinant MccJ25 against foodborne pathogens. Here, the activity of recombinant MccJ25 was examined using a matrix of conditions in order to assess the efficacy of recombinant MccJ25 as a mitigation against foodborne pathogens, such as Salmonella species and Escherichia coli ( E. coli ) O157:H7. Results showed that recombinant MccJ25 displayed excellent antimicrobial activity against these foodborne pathogens, including clinical isolates of Salmonella and E. coli , as well as clinical antibiotic-resistant Salmonella and E. coli isolates with different minimal inhibitory concentrations. In addition, antimicrobial activity curves and Live/Dead assay evidenced that recombinant MccJ25 harbors strong bactericidal activity against Salmonella and E. coli O157:H7. Notably, recombinant MccJ25 also had great potency and induced fast mortality against different growth phase of Salmonella and E. coli. The stability analysis results showed that the activity of recombinant MccJ25 was not influenced by temperatures as high as 121°C. Varying the pH from 2.0 to 9.0 did not appear to affect the activity of recombinant MccJ25. Under the challenge of several proteases, simulated gastrointestinal fluids and serum, recombinant MccJ25 still maintained exceptionally strong antimicrobial activity. Significant reductions in Salmonella Pullorum levels were also achieved in food biological environments, such as milk, egg and meat. Moreover, we demonstrated that recombinant MccJ25 appeared to act by inducing membrane breaks, thinning, and disintegration in the Salmonella Pullorum cytoplasmic membrane. Taken together, these results indicated that recombinant MccJ25 could be an effective alternative for mitigating and prevention of Salmonella and E. coli infection in food, animal and agriculture applications.

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Section: Introductionmentioning

confidence: 99%

A Comprehensive Antimicrobial Activity Evaluation of the Recombinant Microcin J25 Against the Foodborne Pathogens Salmonella and E. coli O157:H7 by Using a Matrix of Conditions

Zeng

et al. 2019

Front. Microbiol.

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“…Due to a previous report on the antimicrobial activity of CM against E. coli, an MIC for CM was first tested against the host E. coli. We also determined the MIC of CM for Salmonella serovar Montevideo to compare the antibacterial efficacy of our product with the same formulation reported elsewhere [43]. In our study, the respective MICs for the E. coli and Salmonella were 0.006% and 0.008% (w/v).…”

Section: Determining the Impact Chitosan Microparticles On Human Noromentioning

confidence: 89%

“…Chitosan is an environmentally friendly carbohydrate biopolymer and is recognized for delivering a broad in vivo and in vitro antibacterial activity. For example, CM has been successfully applied for the mitigation of Salmonella in agricultural water [39,42,43]. The antiviral activity of CM was tested against murine norovirus and bacteriophage MS2 in PBS (pH 7.4).…”

Section: Discussionmentioning

confidence: 99%

“…Studies investigating the impact of chitosan on stability of human norovirus surrogates have generated mixed results, and the effectiveness of the compound may be linked to particle size [40,41]. Recent advancements in generating chitosan particles at micro and nanoscales has enabled the successful in vivo and in vitro elimination of pathogenic bacteria in cattle [42] and in crop irrigation ponds [43]. Due to these enhanced properties, it was hypothesized that chitosan microparticles (CM) would be more effective against noroviruses than previously studied formulations of chitosan.This study investigated the impact of physical and chemical characteristics of surface water including ammonium, phosphate, and ultra-violet light, on the stability of murine norovirus in water at various temperatures.…”

mentioning

confidence: 99%

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Survival of Human Norovirus Surrogates in Water upon Exposure to Thermal and Non-Thermal Antiviral Treatments

Zhu

Barnes

Bhar

et al. 2020

Viruses

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Human noroviruses are the leading cause of foodborne gastroenteritis worldwide and disease outbreaks have been linked to contaminated surface waters as well as to produce consumption. Noroviruses are extremely stable in water and their presence is being detected with increasing frequency, yet there are no viable methods for reducing norovirus contamination in environmental water. Despite this, there is little knowledge regarding the physical and chemical factors that influence the environmental persistence of this pathogen. This study evaluated the impact of common chemical and physical properties of surface water on the stability of murine norovirus and examined the effect of food-safe chitosan microparticles on infectivity of two human norovirus surrogates. While chemical additives had a minor impact on virus survival, chitosan microparticles significantly reduced infectious titers of both murine norovirus and MS2 bacteriophage.

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“…Naturally, degradable materials mostly include polysaccharides and proteins, which are not only cheap and easy to obtain, but may also degrade by chemical or microbiological processes without causing significant environmental pollution . Besides, the degradation of some natural materials may also facilitate the production of crops and increase the production output . Therefore, naturally degradable materials as a pesticide carrier may effectively solve the carrier residue problem of new pesticide formulations.…”

Section: Introductionmentioning

confidence: 99%

Elaboration of a feather keratin/carboxymethyl cellulose complex exhibiting pH sensitivity for sustained pesticide release

Lin

Chen

Zhou

et al. 2018

J of Applied Polymer Sci

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Feather keratin (FK) and carboxymethyl cellulose (CMC) were used as raw materials to prepare a FK/CMC polyelectrolyte complex via electrostatic interactions. Using avermectin (AVM) as a model drug and elevated temperature, an FK/CMC@AVM drugcarrying complex was obtained. The structure and morphology of FK/CMC@AVM were both analyzed by Fourier transform infrared spectroscopy, dynamic light scattering, and scanning electron microscopy (SEM). Furthermore, the encapsulation efficiency, anti-ultraviolet, sustained release, and toxicity properties of FK/CMC@AVM were studied. The results showed that the average particle size of FK/CMC@AVM was 386.57 nm and the encapsulation efficiency was 67.06%. Under UV light irradiation, FK/CMC@AVM significantly improved the stability of AVM and the half-life of AVM was found to be delayed from 354 to 1800 min. Moreover, the sustained release of AVM featured pH sensitivity and was consistent with the Korsmeyer-Peppas model. Upon increasing the pH from 1.5 to 9.5, the release mechanism of AVM changed from Fick diffusion to non-Fick diffusion. Finally, the toxicity characteristics of FK/CMC@AVM were not significantly different from those of nonmodified AVM.

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Application of chitosan microparticles for mitigation of Salmonella in agricultural water

Abstract: This research indicated that CM, a natural compound with minimal environmental impact, could be an effective alternative for mitigating Salmonella in agricultural water applications.

Cited by 11 publications

References 41 publications

A Comprehensive Antimicrobial Activity Evaluation of the Recombinant Microcin J25 Against the Foodborne Pathogens Salmonella and E. coli O157:H7 by Using a Matrix of Conditions

A Comprehensive Antimicrobial Activity Evaluation of the Recombinant Microcin J25 Against the Foodborne Pathogens Salmonella and E. coli O157:H7 by Using a Matrix of Conditions

Survival of Human Norovirus Surrogates in Water upon Exposure to Thermal and Non-Thermal Antiviral Treatments

Elaboration of a feather keratin/carboxymethyl cellulose complex exhibiting pH sensitivity for sustained pesticide release

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