Application of CFD in Bioprocessing: Separation of mammalian cells using disc stack centrifuge during production of biotherapeutics

Shekhawat, Lalita Kanwar; Sarkar, Jayati; Gupta, Rachit; Hadpe, Sandeep Ramesh; Rathore, Anurag S.

doi:10.1016/j.jbiotec.2017.12.016

Cited by 22 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…where r m is mixed density, r=(∂=∂x)i + (∂=∂y)j + (∂=∂z)k is a Hamiltonian (i, j, k are the unit vectors in the axis directions of x, y, z, respectively), v * m is the mass average velocity, _ m, which can be zero unless otherwise specified, is a user-defined mass source or the mass transfer caused by cavitation. [54][55][56] The momentum equation for the mixed phase is…”

Section: Mixture Multiphase Modelmentioning

confidence: 99%

An ultrasonic-assisted soft abrasive flow processing method for mold structured surfaces

Zhu

2019

Advances in Mechanical Engineering

View full text Add to dashboard Cite

As a fluid-based precise processing method, soft abrasive flow processing has been widely used in advanced electromechanical systems, complex mold manufacturing, and other engineering fields. Because of the low volume fraction of abrasive particles and micro-force/cutting removal characteristics, there exists a potential improvement in terms of processing efficiency and uniformity. In view of the above problems, this article presents an ultrasonic-assisted soft abrasive flow processing method. Based on the realizable k–ε turbulence model and the mixture flow model, an ultrasonic coupling enhancement dynamic model for soft abrasive flow is set up, and the kinetic energy transport equation of realizable k–ε turbulence model can be revised. Using particle image velocimetry technology, an on-line observation experimental platform for ultrasonic-assisted soft abrasive flow is developed to conduct the real-time acquisition of abrasive flow state and particle distribution in a constrained flow passage. An ultrasonic-assisted soft abrasive flow processing experimental platform is established to complete the processing experiment. The experimental results show that the ultrasonic excitation vibration can effectively enhance the turbulence intensity and distribution uniformity of the abrasive flow, the average processing time can be shortened by more than 6 h, and a better surface quality can be obtained.

show abstract

Section: Mixture Multiphase Modelmentioning

confidence: 99%

An ultrasonic-assisted soft abrasive flow processing method for mold structured surfaces

Zhu

2019

Advances in Mechanical Engineering

View full text Add to dashboard Cite

show abstract

“…Germany) 26; 55 45 38.5 ( Espuny, 2016 ) Frau CN2S Frau (Italy) 15; 54.5 51 52 ( Jukkola et al., 2019 ) Culturefuge 100™ Alfa Laval AB (Lund. Sweden) 36; 84.5 82 8 0.004 40 ( Shekhawat et al., 2018 ) …”

Section: Methodsmentioning

confidence: 99%

White-flesh guava juice clarification by a fixed-angle conical rotor centrifuge laboratory and characterization of continuous disk stack centrifuges

Ninga

Desobgo

Nso

et al. 2022

Heliyon

View full text Add to dashboard Cite

“…In industrial centrifugation, conical designs such as cone-stack type centrifuge [39,40], and conical plate centrifuge [41] are prevalent. In subsequent designs (C-E), we adopted a truncated cone shape design to achieve a drag coefficient to 0.05-0.5 and thus reduce the aerodynamic drag force operating around the rotor body.…”

Section: R9mentioning

confidence: 99%

A low-cost, open-source centrifuge adaptor for separating large volume clinical blood samples

et al. 2022

View full text Add to dashboard Cite

Blood plasma separation is a prerequisite in numerous biomedical assays involving low abundance plasma-borne biomarkers and thus is the fundamental step before many bioanalytical steps. High-capacity refrigerated centrifuges, which have the advantage of handling large volumes of blood samples, are widely utilized, but they are bulky, non-transportable, and prohibitively expensive for low-resource settings, with prices starting at $1,500. On the other hand, there are low-cost commercial and open-source micro-centrifuges available, but they are incapable of handling typical clinical amounts of blood samples (2-10mL). There is currently no low-cost CE marked centrifuge that can process large volumes of clinical blood samples on the market. As a solution, we customised the rotor of a commercially available low-cost micro-centrifuge (~$125) using 3D printing to enable centrifugation of large clinical blood samples in resource poor-settings. Our custom adaptor ($15) can hold two 9 mL S-Monovette tubes and achieve the same separation performance (yield, cell count, hemolysis, albumin levels) as the control benchtop refrigerated centrifuge, and even outperformed the control in platelet separation by at least four times. This low-cost open-source centrifugation system capable of processing clinical blood tubes could be valuable to low-resource settings where centrifugation is required immediately after blood withdrawal for further testing.

show abstract

Application of CFD in Bioprocessing: Separation of mammalian cells using disc stack centrifuge during production of biotherapeutics

Cited by 22 publications

References 33 publications

An ultrasonic-assisted soft abrasive flow processing method for mold structured surfaces

An ultrasonic-assisted soft abrasive flow processing method for mold structured surfaces

White-flesh guava juice clarification by a fixed-angle conical rotor centrifuge laboratory and characterization of continuous disk stack centrifuges

A low-cost, open-source centrifuge adaptor for separating large volume clinical blood samples

Contact Info

Product

Resources

About