Application of centrifugal microfluidics in immunoassay, biochemical analysis and molecular diagnosis

Shi, Yan; Peng, Ye; Yang, Kang; Meng, Jie; Jie, Guo; Pan, Zhixiang; Zhao, Wenhao; Guo, Jinhong

doi:10.1039/d1an00629k

Cited by 34 publications

(20 citation statements)

References 95 publications

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“…[46] The detailed description of the CD microfluidic system are as follows. [47][48][49] Cohen et al proposed a microsphere-based assay to detect dynamic changes in the concentration of anti-TNF-α antibodies, without the washing steps required by standard immunoassays. [50] In another study, a microfluidic chip electrophoresis assay was more effective than traditional SDS-PAGE in determining the purity and size of IgG in hybridoma cell cultures.…”

Section: Detection and Analysis Of Hybridoma Cellsmentioning

confidence: 99%

Application of microfluidic technology in antibody screening

Wang

2022

Biotechnology Journal

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Specific antibodies are widely used in the biomedical field. Current screening methods for specific antibodies mainly involve hybridoma technology and antibody engineering techniques. However, these technologies suffer from tedious screening processes, long preparation periods, high costs, low efficiency, and a degree of automation, which have become a bottleneck for the screening of specific antibodies. To overcome these difficulties, microfluidics has been developed as a promising technology for high‐throughput screening and high purity of antibody. In this review, we provide an overview of the recent advances in microfluidic applications for specific antibody screening. In particular, hybridoma technology and four antibody engineering techniques (including phage display, single B cell antibody screening, antibody expression, and cell‐free protein synthesis) based on microfluidics have been introduced, challenges, and the future outlook of these technologies are also discussed.

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Section: Detection and Analysis Of Hybridoma Cellsmentioning

confidence: 99%

Application of microfluidic technology in antibody screening

Wang

2022

Biotechnology Journal

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“…The developed AI app successfully recognized various fluidic states in the reaction chamber and appropriately actuated the microfluidic device for performing ELISA by resolving fluid errors such as bubbles and incomplete filling in the reaction space. Compared to the manually controlled microfluidic immunoassay that already offers improved results than the conventional ELISA, the AI-powered automated microfluidic platform provided better repeatability by reducing the overlapping of the test results with the other concentrations result due to high standard deviation, which is significantly influential to the assay outcomes and contributing to the lower limit of detection (LOD) for diagnosing cTnI. ,− Thus, the clinical application of this proposed device may have a great impact for replacing the manual method by AI for clinical applications and commercialization, also saving lives by diagnosing cardiac diseases in a very early stage.…”

Section: Introductionmentioning

confidence: 99%

Artificial Intelligence-Controlled Microfluidic Device for Fluid Automation and Bubble Removal of Immunoassay Operated by a Smartphone

et al. 2022

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There have been tremendous innovations in microfluidic clinical diagnostics to facilitate novel point-of-care testing (POCT) over the past decades. However, the automatic operation of microfluidic devices that minimize user intervention still lacks reliability and repeatability because microfluidic errors such as bubbles and incomplete filling pose a major bottleneck in commercializing the microfluidic devices for clinical testing. In this work, for the first time, various states of microfluid were recognized to control immunodiagnostics by artificial intelligence (AI) technology. The developed AI-controlled microfluidic platform was operated via an Android smartphone, along with a low-cost polymer device to effectuate enzyme-linked immunosorbent assay (ELISA). To overcome the limited machine-learning capability of smartphones, the region-of-interest (ROI) cascading and conditional activation algorithms were utilized herein. The developed microfluidic chip was incorporated with a bubble trap to remove any bubbles detected by AI, which helps in preventing false signals during immunoassay, as well as controlling the reagents' movement with an on-chip micropump and valve. Subsequently, the developed immunosensing platform was tested for conducting real ELISA using a single microplate from the 96-well to detect the Human Cardiac Troponin I (cTnI) biomarker, with a detection limit as low as 0.98 pg/mL. As a result, the developed platform can be envisaged as an AI-based revolution in microfluidics for point-of-care clinical diagnosis.

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“…Among them, the centrifugal microfluidic immunoassay system plays a great role in disease prevention and diagnosis. However, different from the traditional laboratory, the centrifugal microfluidic immunoassay system is based on the microchannel network, which integrates all steps, including fluid loading, reaction and detection onto a "lab-on-a-chip" where precise step-by-step liquid transfer is required [4,5]. Then, the performance of the liquid flow on the chip greatly affects their rapidity and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System

Shi

Wang

et al. 2022

Micromachines

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The surface of a centrifugal microfluidic immunoassay system chip such as polymethyl methacrylate (PMMA) is often hydrophobic, which leads to problems such as poor liquid transfer efficiency and easy-to-block siphon channels, leading to bad fluid control. Therefore, surface hydrophilic modification for such chips is necessary to improve the rapidity and sensitivity of the system. Chemical modification is commonly used, but there is little research on the hydrophilic effect of different concentrations of hydrophilic reagents. According to function requirements for different microchannels of the chip (some only need to ensure the liquid can flow into the next chamber, and some also need to ensure the function of “closing the door” during immunoassay incubation), we explored the best combination of hydrophilic reagent and concentration through experiments. Firstly, three hydrophilic reagents were used for modification. Secondly, the hydrophilic effects of different reagents and concentrations were explored by contact angle test, the influence of different modification methods on liquid transfer efficiency was characterized by residual liquid calculation in the chamber. Finally, the effect of different hydrophilic reagents on absorbance was also tested. By experimental results and comprehensively considering the stability of the modification effect and the function requirements, Tween-20 (2.0% v/v) was chosen as the modifying reagents of the first siphon valve and the second siphon valve, and TritonX-100 (2.0% v/v) was chosen for the third siphon valve, which effectively reduces the contact angle and improves the liquid transfer efficiency, leading to further improvement of the rapidity and sensitivity of the centrifugal microfluidic immunoassay system by efficient siphoning and high plasma separation efficiency (99%).

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Application of centrifugal microfluidics in immunoassay, biochemical analysis and molecular diagnosis

Abstract: Rapid diagnosis plays a vital role in daily life, and is effective in reducing treatment costs and increasing curability, especially in remote areas with limited availability of resources. Among the...

Cited by 34 publications

References 95 publications

Application of microfluidic technology in antibody screening

Application of microfluidic technology in antibody screening

Artificial Intelligence-Controlled Microfluidic Device for Fluid Automation and Bubble Removal of Immunoassay Operated by a Smartphone

Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System

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