Application of centrifugal force to the extraction and separation of parasorboside and gerberin from Gerbera hybrida

Abstract

A method for the separation of parasorboside and gerberin from the ornamental plant Gerbera hybrida (Asteraceae) has been developed. The two closely related glucosides were extracted using an Extrachrom instrument, a prototype multi-functional separation tool equipped with an extraction chamber. The rotation planar extraction procedure was compared with that of a medium pressure solid-liquid extraction system. The resulting extracts were pre-purified using rotation planar chromatography and the results compared with those obtained using medium pressure liquid chromatography with silica gel as the stationary phase and a mobile phase of methanol:ethyl acetate:tetrahydrofuran at selectivity point Ps = 111 with 1% formic acid as modifier. The title compounds were isolated from the purified extracts by TLC and their structures confirmed by 1H- and 13C-NMR spectroscopy.

Comparison of Medium Pressure Solid–Liquid Extraction and Rotation Planar Extraction of Ficus Leaves with Reference to Optimum Operating Parameters

“…In the extraction of floral stems and leaves of Gerbera hybrida, the two methods have previously been shown to be practically equal in terms of extraction yield and the quality of the extracts, when the number of extraction cycles was the same for both methods. [6] The two extraction methods seemed equal in terms of the volume of solvent used for the extraction. Increasing the volume of solvent led to a slight increase in the extraction yield but, at the same time, the amount of nonpolar components in the extract decreased.…”

“…The construction of the separation instrument has been described previously. [6] Dried and milled leaves of F. bengalensis L. were used as the plant material and were extracted with chloroform. The medium particle size and volume of solvent were chosen for the operating variables on the basis of the results of the MPSLE method (Table 1).…”

“…So far, only few studies concerning the use of RPE in the extraction of plant material have been published. [6][7][8] In this investigation, a factorial experimental design with three levels of three independent operating variables was applied for studying the MPSLE process and for predicting the influence of the operating variables on the extraction yield. The dependences of the extraction yield, the amount of nonpolar compounds in the extract, and the amount of nonpolar compounds obtained from the lower segment of the column on the three independent operating variables, were estimated using multilinear stepwise regression analysis.…”

Gerberan karvasaineet suojaavat hyönteisherbivorialta

“…Kasvien gerberiini-ja parasorbosidipitoisuudet mitattiin uuttamalla pakastekuivatut lehtinäytteet metanolilla ja analysoimalla uutteet ohutlevykromatografialla (Yrjönen et al 2002). Glukosidit tunnistettiin kastamalla levy 15% rikkihappoon metanolissa ja lämmittämällä levyä 10 min 120 °C.…”

Preparation and Application of Reversed Phase ChromatorotorTM for the Isolation of Natural Products by Centrifugal Preparative Chromatography

“…The method was later modified for adsorption chromatography [6][7][8][9] and was further developed and renamed as rotation planar chromatography (RPC) in the 1980s by Nyiredy [10,11], which catered for analytical, micro-preparative and preparative separations [12,13]. CPC is currently applied to preparative work using commercially available instruments, Chromatotron ® [14], Cyclograph ® [15], and Rotachrom P [16], while ExtraChrom [17], another RPC instrument used for preparative work is not available commercially.…”