Application of Cationic Colloidal Gold Staining for the Demonstration of Anionic Sites.

Saga, Kenji

doi:10.1267/ahc.31.1

Cited by 4 publications

(5 citation statements)

References 30 publications

(52 reference statements)

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“…Distribution of anionic sites Cationic colloidal gold labels net negative charge on the histologic sections (Skutelsky and Roth, 1986;Saga, 1998). Therefore, an alteration of pH has a marked effect on the labeling with cationic gold.…”

Section: Markers To Be Used For the Differentiation Of Eccrine And Apocrine Sweat Glandsmentioning

confidence: 99%

“…Charge theory alone does not explain this paradoxical pHdependency observed in apocrine sweat glands. It is speculated that an effect of low pH may be to alter the structural con®guration of sialoprotein, thus rendering charge sites available for labeling with cationic colloidal gold (Saga and Takahashi, 1993;Saga, 1998).…”

Section: Markers To Be Used For the Differentiation Of Eccrine And Apocrine Sweat Glandsmentioning

confidence: 99%

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Histochemical and Immunohistochemical Markers for Human Eccrine and Apocrine Sweat Glands: An Aid for Histopathologic Differentiation of Sweat Gland Tumors

Saga

2001

Journal of Investigative Dermatology Symposium Proceedings

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Apocrine and eccrine sweat glands are distinct in function, although they are closely related to each other developmentally and morphologically. In certain sweat gland tumors, it is difficult to differentiate between eccrine or apocrine sweat glands. Therefore, this paper reviews histochemical and immunohistochemical markers to differentiate apocrine and eccrine sweat glands with the aim of better understanding the structural and functional characteristics of these sweat glands. Specific markers for apocrine sweat glands are as follows: neuraminidase sensitive anionic sites detected by cationic colloidal gold at pH 2.0, and mitochondrion-like secretory granules that have epidermal growth factor-like antigenicity. The following antibodies react with apocrine sweat glands but not with eccrine sweat glands; the antibodies raised against 70 kDa glycoprotein purified from human milk fat globule membranes, and HMFG-1 (1.10.F3) monoclonal antibody produced by immunizing mice with defatted human milk fat globule membranes. Markers for eccrine sweat glands are as follows: dark cell granules that have chondroitinase ABC sensitive anionic sites detected by cationic gold at pH 2.0 after pretreatment with EGTA, and intercellular canaliculi with high activity of alkaline phosphatase. CEA and GCDFP-15 are expressed in both eccrine and apocrine sweat glands. Anti-EMA monoclonal antibody (E29) stains both eccrine and apocrine sweat glands.

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Section: Markers To Be Used For the Differentiation Of Eccrine And Apocrine Sweat Glandsmentioning

confidence: 99%

Section: Markers To Be Used For the Differentiation Of Eccrine And Apocrine Sweat Glandsmentioning

confidence: 99%

Histochemical and Immunohistochemical Markers for Human Eccrine and Apocrine Sweat Glands: An Aid for Histopathologic Differentiation of Sweat Gland Tumors

Saga

2001

Journal of Investigative Dermatology Symposium Proceedings

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“…Therefore demonstration of sialomucin by conventional mucin staining [4,18] does not determine the direction of differentiation in Paget cells. Positively charged colloidal gold stains anionic sites on the sections embedded in the hydrophilic resins [14,20,24]. At pH 2.0, cationic gold labels sialic acid and sulfated glycosaminoglycans such as chondroitin sulfate, dermatan sulfate, and heparan sulfate [14,22].…”

Section: Discussionmentioning

confidence: 99%

“…Positively charged colloidal gold stains anionic sites on the sections embedded in the hydrophilic resins [14,20,24]. At pH 2.0, cationic gold labels sialic acid and sulfated glycosaminoglycans such as chondroitin sulfate, dermatan sulfate, and heparan sulfate [14,22]. We have previously reported that eccrine and apocrine sweat glands showed different distribution of anionic sites and different susceptibility to enzyme digestion [16].…”

Section: Discussionmentioning

confidence: 99%

Localization and Characterization of Anionic Sites in Extramammary Paget's Disease with Cationic Colloidal Gold.

Saga

Jimbow

1999

Acta Histochem. Cytochem

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“…Provided suitable probes are available, scanning electron microscopy (SEM) unquestionably would be a more adequate approach to provide an overall picture of the sperm surface and to address this issue. Colloidal gold has been introduced in recent years as a cationic probe (Kashio et al 1992;Ohtsuka and Murakami 1994;Saga 1998;Skutelsky and Roth 1986;Skutelsky et al 1995). This lead us to attempt to track the anionic charges by means of cationised gold and using the backscattered electron signal in SEM.…”

Section: Introductionmentioning

confidence: 99%

Demonstration and cytochemical analysis of anionic sites on ejaculated boar spermatozoa: a scanning electron microscopy study using cationised colloidal gold

Stoffel

Frieß

2001

Histochem Cell Biol

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The plasmalemma of spermatozoa bears negative charges as is the case for most mammalian cells. This has been concluded from the sperm cell's electrophoretic behaviour and from labelling experiments with various cationic probes followed by transmission electron microscopy of ultrathin sections. An overall view of the cell surface, however, is necessary in order to assess the distribution and density of the anionic sites adequately. We, therefore, used scanning electron microscopy in combination with cationised colloidal gold labelling to analyse the presence of anionic sites on ejaculated boar spermatozoa. Incubations were performed at pH 3.5, 2.5 and 1.0. Labelling was specific and bound gold particles were unequivocally identified using the backscattered electron signal. The chemical nature of the anionic sites involved was investigated by treating spermatozoa with pronase, phosphatase and neuraminidase as well as by methylation, acid hydrolysis and beta-elimination prior to cationised gold labelling. Our results suggest that besides phosphates, carboxyl groups are predominantly accountable for the binding of cationised colloidal gold. Presumptive macromolecules bearing these anionic sites are phospholipids and sialic acid residues. The combination of methods presented herewith should be of value in order to elucidate charge interactions which have been shown to play a role in cellular recognition events and adhesion.

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Application of Cationic Colloidal Gold Staining for the Demonstration of Anionic Sites.

Cited by 4 publications

References 30 publications

Histochemical and Immunohistochemical Markers for Human Eccrine and Apocrine Sweat Glands: An Aid for Histopathologic Differentiation of Sweat Gland Tumors

Histochemical and Immunohistochemical Markers for Human Eccrine and Apocrine Sweat Glands: An Aid for Histopathologic Differentiation of Sweat Gland Tumors

Localization and Characterization of Anionic Sites in Extramammary Paget's Disease with Cationic Colloidal Gold.

Demonstration and cytochemical analysis of anionic sites on ejaculated boar spermatozoa: a scanning electron microscopy study using cationised colloidal gold

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