Application of carbocyanine fluorescent probes for evaluation of the functional state of cell cultures after cryoconservation

Abstract

Èññëå äî âà íà âîç ìîae íîñòü ïðè ìå íå íèÿ êàð áî öè à íè íî âûõ ôëó î ðåñ öåí òíûõ çîí äîâ Ñ2, Ñ9 è JC-1 äëÿ õà ðàê òå ðèñ òè êè êðè î êîí ñåð âè ðî âàí íûõ êëå òîê äèï ëî èä íîé ëè íèè ôèá ðîá ëàñ òîâ ÷å ëî âå êà. Ïî êà çàíî, ÷òî ýòè çîí äû ìî ãóò áûòü èñ ïîëü çî âà íû ïðè êðè î êîí ñåð âè ðî âà íèè êëå òî÷ íûõ ëè íèé äëÿ ìî íèòî ðèí ãà èõ ôóíê öè î íàëü íî ãî ñî ñòî ÿ íèÿ. Êëþ ÷å âûå ñëî âà: ôëó î ðåñ öåí öèÿ, çîí äû, ôèá ðîá ëàñ òû ÷å ëî âå êà, êóëü òó ðà êëå òîê, êðè î êîí ñåð âè ðîâà íèå.

Newly synthesized carbocyanine fluorescent probes, their characteristics and behavior in proliferating cultures

“…We have synthesized and investigated the carbocyanine dyes differing in their hydrophilic-hydrophobic features. As it has been reported [1] the staining with these dyes was performed prior to investigating and there are only single papers on culturing cell lines with integrated dyes [2,3]. The research was aimed at comparative characterization of the behavior of syn-thesized by us probes in proliferating cell cultures.…”