Application of Capillary Electrophoresis for High‐Throughput Screening of Drug Metabolism

Řemínek, Roman; Pauwels, Jochen; Wang, Xu; Hoogmartens, Jos; Glatz, Zdeněk; Schepdael, Ann Van

doi:10.1002/9781118530009.ch17

Cited by 2 publications

(2 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CYP3A4 in the purchased SUPERSOMES is membrane bound and probably has a lower diffusion coefficient than albumin. Based on efforts dealing with CYP2C9 SUPERSOMES, a diffusion coefficient of 1.34 × 10 -11 m 2 /s was reported for that system [29]. Using this value instead of 5.94 × 10 -11 m 2 /s resulted in an even broader Aris-Taylor dispersion (red broken line graph in Fig.…”

Section: Simulation Of Diffusional Mixing During Plug Injectionmentioning

confidence: 95%

“…For the investigations made in our work, an exact value is not required as spreading of the protein is sufficient for both cases. The diffusion coefficient of NADPH used (64.21 × 10 -11 m 2 /s) corresponds to the value given in [29] and was also employed for ketamine. These values may not be accurate but they provide sufficient insight into the principle of mixing by transverse diffusion when plugs are inserted with a specified velocity.…”

Section: Simulation Of Diffusional Mixing During Plug Injectionmentioning

confidence: 99%

See 1 more Smart Citation

Simulation and experimental study of enzyme and reactant mixing in capillary electrophoresis based on-line methods

Pelcová

Řemínek

Sandbaumhüter

et al. 2016

Journal of Chromatography A

Self Cite

View full text Add to dashboard Cite

The establishment of an efficient reaction mixture represents a crucial part of capillary electrophoresis based on-line enzymatic assays. For ketamine N-demethylation to norketamine mediated by the cytochrome P450 3A4 enzyme, mixing of enzyme and reactants in the incubation buffer at physiological pH was studied by computer simulation. A dynamic electrophoretic simulator that encompasses Taylor-Aris diffusivity which accounts for dispersion due to the parabolic flow profile associated with pressure driven flow was utilized. The simulator in the diffusion mode was used to predict transverse diffusional reactant mixing occurring during hydrodynamic plug injection of configurations featuring four and seven plugs. The same simulator in the electrophoretic mode was applied to study electrophoretic reactant mixing caused by voltage application in absence of buffer flow. Resulting conclusions were experimentally verified with enantioselective analysis of norketamine in a background electrolyte at low pH. Furthermore, simulations visualize buffer changes that occur upon power application between incubation buffer and background electrolyte and have an influence on the reaction mixture.

show abstract

Section: Simulation Of Diffusional Mixing During Plug Injectionmentioning

confidence: 95%

Section: Simulation Of Diffusional Mixing During Plug Injectionmentioning

confidence: 99%

Simulation and experimental study of enzyme and reactant mixing in capillary electrophoresis based on-line methods

Pelcová

Řemínek

Sandbaumhüter

et al. 2016

Journal of Chromatography A

Self Cite

View full text Add to dashboard Cite

show abstract

Recent advances in CE‐mediated microanalysis for enzyme study

Wang

Adams

et al. 2013

Electrophoresis

Self Cite

View full text Add to dashboard Cite

This review gives an overview of the recent developments and applications in the use of CE-mediated microanalysis for enzyme studies. The period covers mid-2011 until mid-2013. Both off-line and in-line enzyme assays with their applications using CE are described in this article. For the in-capillary enzyme reaction, the techniques using electrophoretically mediated microanalysis (EMMA) as well as immobilized enzyme reactor (IMER) are discussed. The applications include the evaluation of enzyme activity, enzyme kinetics, enzyme inhibition, screening of enzyme inhibitors, and the study of enzyme-mediated drug metabolism.

show abstract

Application of Capillary Electrophoresis for High‐Throughput Screening of Drug Metabolism

Cited by 2 publications

References 52 publications

Simulation and experimental study of enzyme and reactant mixing in capillary electrophoresis based on-line methods

Simulation and experimental study of enzyme and reactant mixing in capillary electrophoresis based on-line methods

Recent advances in CE‐mediated microanalysis for enzyme study

Contact Info

Product

Resources

About