Application of bovine microsatellite markers on Saola (Pseudoryx nghetinhensis)

Abstract

The aim of the present study was to assess the applicability of bovine microsatellite markers on Saola (Pseudoryx nghetinhensis). A total of 127 microsatellite markers were tested on a male and a young female Saola. An efficient amplification was observed for 123 markers (96.8%), 73 markers (59.3%) were polymorphic. Four loci (BM2304, BMS1928, BMS779 and ILSTS006) on cattle chromosomes 1, 4, 7 and 8, respectively, failed to amplify in Saola. Two cattle Y-chromosome-specific microsatellite markers (INRA126 and BM861) were successfully amplified from both sexes in Saola. However, two additional markers (INRA124 and INRA189) on Y-chromosome failed to amplify in the female animal. These results show that most of the bovine microsatellite markers are applicable in Saola and therefore they can be used to study the phylogenetic relationships and the genetic diversity of the Saola population.

Genomic conservation of cattle microsatellite loci in wild gaur (Bos gaurus) and current genetic status of this species in Vietnam

“…This could be attributed to the absence of homologous sequences in both species. Moreover, studies demonstrated that BM861 and INRA126 successfully amplified from both sexes in yak [ 18 , 21 ] and saola ( Pseudoryx nghetinhensis – [ 20 ]), suggesting that they are not Y-specific. These findings indicated that yak and saola X chromosome retained a homologous segment of the Y chromosome, which contains both BM861 and INRA126 microsatellite markers.…”

“…Successful amplification and extensive conservation of cattle microsatellite sequences in several species of Bovidae and Cervidae families have been documented in numerous works [ 13 , 14 ], thus allowing possible population genetic studies on related Bovidae species for which microsatellites have not been developed [ 15 - 18 ]. Furthermore, cross-species amplification was also applied to the study of population variations in geographically isolated or endangered species [ 19 , 20 ]. These studies suggest that a characterization of wild gaur, as a member of the subfamily Bovinae, with bovine microsatellite markers is highly pertinent and suitable.…”

Genetic structure analysis of a highly inbred captive population of the African antelope Addax nasomaculatus. Conservation and management implications

“…Being phylogenetically very close to A. nasomaculatus , the results obtained are comparable and similar to the ones presented here. Nguyen et al [2005] analyzed 127 bovine microsatellites in a male and a female of the Vietnamese antelope saola ( Pseudoryx nghetinhensis ), achieving a successful amplification of 96.8% of the markers. All these results show a close relationship between wild antelopes and domestic bovids, and the usefulness of markers developed for well‐known species for characterization and monitoring of endangered ones.…”

“…Thirteen microsatellites amplified successfully, but only three were found to be polymorphic. At present, there are very few studies that have used molecular markers in related species [Marshall et al, 1999; Nguyen et al, 2005].…”