Application of bottom‐up and top‐down proteomics in
            <i>Medicago</i>
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Gutsch, Annelie; Sergeant, Kjell; Renaut, Jenny

doi:10.1002/9781119409144.ch141

Cited by 1 publication

(1 citation statement)

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“…Mass spectroscopy is central to large-scale proteome analysis that enhances the resolution, sensitivity, and accuracy of proteins mass prediction ( Cassidy et al, 2021 ). Due to these and the speed of analysis for large protein samples through released peptides after proteolytic digestion (bottom-up), shotgun proteomics is used to describe the process ( Gutsch et al, 2019b ). On the other hand, protein is partially digested to characterize co-existing post-translational modifications (PTMs; Sidoli et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Understanding the Phytoremediation Mechanisms of Potentially Toxic Elements: A Proteomic Overview of Recent Advances

et al. 2022

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Potentially toxic elements (PTEs) such as cadmium (Cd), lead (Pb), chromium (Cr), and arsenic (As), polluting the environment, pose a significant risk and cause a wide array of adverse changes in plant physiology. Above threshold accumulation of PTEs is alarming which makes them prone to ascend along the food chain, making their environmental prevention a critical intervention. On a global scale, current initiatives to remove the PTEs are costly and might lead to more pollution. An emerging technology that may help in the removal of PTEs is phytoremediation. Compared to traditional methods, phytoremediation is eco-friendly and less expensive. While many studies have reported several plants with high PTEs tolerance, uptake, and then storage capacity in their roots, stem, and leaves. However, the wide application of such a promising strategy still needs to be achieved, partly due to a poor understanding of the molecular mechanism at the proteome level controlling the phytoremediation process to optimize the plant’s performance. The present study aims to discuss the detailed mechanism and proteomic response, which play pivotal roles in the uptake of PTEs from the environment into the plant’s body, then scavenge/detoxify, and finally bioaccumulate the PTEs in different plant organs. In this review, the following aspects are highlighted as: (i) PTE’s stress and phytoremediation strategies adopted by plants and (ii) PTEs induced expressional changes in the plant proteome more specifically with arsenic, cadmium, copper, chromium, mercury, and lead with models describing the metal uptake and plant proteome response. Recently, interest in the comparative proteomics study of plants exposed to PTEs toxicity results in appreciable progress in this area. This article overviews the proteomics approach to elucidate the mechanisms underlying plant’s PTEs tolerance and bioaccumulation for optimized phytoremediation of polluted environments.

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Section: Introductionmentioning

confidence: 99%