Application of bis-eugenol to a zinc oxide eugenol cement

Fujisawa, Seiichiro; Kashiwagi, Yusuke; Atsumi, Toshiko; Iwakura, I; Ueha, T; Hibino, Y.; Yokoe, Ichiro

doi:10.1016/s0300-5712(98)00056-6

Cited by 30 publications

(33 citation statements)

References 14 publications

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“…HGF were isolated, as described previously. 9) Briefly, gingival tissues were obtained from healthy gingival biopsies from a 10-year-old girl, undergoing periodontal surgery. The tissue was cut into 1 to 2 mm 3 pieces, washed twice with phosphate-buffered saline (PBS, 0.01 M phosphate buffer, 0.15 M NaCl, pH 7.4) supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin, and placed into 25 cm 2 tissue culture flasks.…”

Section: Methodsmentioning

confidence: 99%

Steroidal Glycosides from the Bulbs of Camassia leichtlinii and Their Cytotoxic Activities.

Kuroda

Mimaki

Hasegawa

et al. 2001

CHEMICAL & PHARMACEUTICAL BULLETIN

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Phytochemical analysis of the bulbs of Camassia leichtlinii (Liliaceae) resulted in the isolation of six new spirostanol saponins, a new furostanol saponin, a cholestane glucoside, and four known steroidal saponins. The structures of the new saponins were determined by detailed analysis of their spectral data, including two-dimensional NMR spectroscopy, and by the results of hydrolytic cleavage. Cytotoxic activities of the isolated compounds against human oral squamous cell carcinoma (HSC-2) cells and normal human gingival fibroblasts (HGF) are also reported.

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Section: Methodsmentioning

confidence: 99%

Steroidal Glycosides from the Bulbs of Camassia leichtlinii and Their Cytotoxic Activities.

Kuroda

Mimaki

Hasegawa

et al. 2001

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…Also, the choice of cell line and HGF or HSG cells used in continuous culture permitted an accurate evaluation of the changes in cytotoxic activity of the dental materials [18]. Also, the choice of cell line and HGF or HSG cells used in continuous culture permitted an accurate evaluation of the changes in cytotoxic activity of the dental materials [18].…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Cytotoxicity and Phospholipid-Liposome Phase-Transition Properties of 2-Hydroxyethyl Methacrylate (Hema)

Fujisawa

Atsumi

Kadoma

2001

Artificial Cells, Blood Substitutes, and Biotechnology

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To elucidate the cytotoxic induction mechanisms of the hydrophilic HEMA, the comparative cytotoxic activities of HEMA and the hydrophobic monomers TEGDMA and MMA were studied, using erythrocytes, gingival fibroblasts and a salivary gland carcinoma cell line. Also, the gel-to-fluid phase transition properties (i.e. temperature, Tm; cooperativity, H/HHW; enthalpy, deltaH) of dipalmitoylphosphatidylcholine (DPPC) and DPPC/cholesterol (CS) liposomes (as a model for biological membranes) induced by methacrylates were investigated, using differential scanning calorimetry (DSC). In addition, the methacrylate-chemical-shifts in DPPC liposomes were assayed using NMR spectroscopy. Both the hemo lytic and cytotoxic activity declined in the order: TEGDMA> HEMA>MMA. The changes in Tm increased in the order: HEMA >TEGDMA, while in contrast, that of HEMA was slightly increased without changes in the deltaH. The DSC changes in DPPC/CS liposomes with HEMA were the largest of those recorded. The cytotoxicity of HEMA may be induced by the hydrophobic interaction derived from the molecular association of OH groups of HEMA and, in addition, by the preferential interaction with CS.

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“…Cytotoxic assay HGF and HSG cells were prepared in the same manner as previously reported (Fujisawa et al, 1999). The MTT[3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay measures succinic dehydrogenase activity, giving an indication of the level of cell respiration.…”

Section: Haemolysis Studymentioning

confidence: 99%

Cytotoxicity of methyl methacrylate (MMA) and related compounds and their interaction with dipalmitoylphosphatidylcholine (DPPC) liposomes as a model for biomembranes

2000

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OBJECTIVE: To clarify the potential mechanism of action of methyl methacrylate (MMA) and related compounds to membranes of living cells, compared with their interaction with dipalmitoylphosphatidylcholine (DPPC) liposomes as a model for biological membranes. MATERIALS AND METHODS: For (meth)acrylates, MMA, ethyl acrylate(EA), n‐butyl acrylate (BA) and n‐butyl methacrylate (BMA) and for living cells, primary human gingival fibroblast (HGF), human submandibular gland adenocarcinoma cell line (HSG) and human erythrocytes were used. The physicochemical changes in DPPC liposomes induced by (meth)acrylates were studied using differential scanning calorimetry (DSC) and nuclear magnetic resonance spectroscopy (NMR). RESULTS: Cytotoxicity decreased as follows: BA>BMA>EA>MMA.Changes in phase transition properties (temperature Tm, enthalpy ΔH and Height/Half‐Height Width (H/HHW) of DSC peak were decreased as follows: BA>EA>MMA. BMA enhanced H/HHW and increased Tm slightly. NMR‐shielding effect decreased as follows: BMA>MMA>BA, EA. CONCLUSION: BA and BMA exhibited large cytotoxicity and high DPPC‐interaction due to their lipophilicity, compared to EA or MMA.MMA showed little cytotoxicity and small changes in DPPC liposomes, whereas BA showed large cytotoxicity and large changes in the liposomes characterized by the membrane disturbance. Hae‐molytic activity and cytotoxicity of acrylates were higher than those of methacrylates. The physico‐chemical properties (Log P or Qσ) of (meth) acrylates affect the lipid bilayer in biological membranes.

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Application of bis-eugenol to a zinc oxide eugenol cement

Cited by 30 publications

References 14 publications

Steroidal Glycosides from the Bulbs of Camassia leichtlinii and Their Cytotoxic Activities.

Steroidal Glycosides from the Bulbs of Camassia leichtlinii and Their Cytotoxic Activities.

Cytotoxicity and Phospholipid-Liposome Phase-Transition Properties of 2-Hydroxyethyl Methacrylate (Hema)

Cytotoxicity of methyl methacrylate (MMA) and related compounds and their interaction with dipalmitoylphosphatidylcholine (DPPC) liposomes as a model for biomembranes

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