Application of biofluid 1H nuclear magnetic resonance-based metabonomic techniques for the analysis of the biochemical effects of dietary isoflavones on human plasma profile

Solanky, Kirty S.; Bailey, Nigel J.; Beckwith-Hall, Bridgette M.; Davis, Adrienne L.; Bingham, Sheila; Holmes, Elaine; Nicholson, Jeremy K.; Cassidy, Aedín

doi:10.1016/j.ab.2003.08.028

Cited by 187 publications

(148 citation statements)

References 16 publications

Supporting

Mentioning

141

Contrasting

Unclassified

Order By: Relevance

“…The first published study in which a metabolomics approach was described in a human nutrition experiment used NMR technology to monitor the effect of supplementing the diet with soy [53]. Only a small number of plasma samples were available and there was considerable inter-person variability but, despite these limitations, careful data pre-processing in combination with powerful discriminatory analysis grouped the samples into two main classes that reflected the dietary intervention.…”

Section: Metabolomics Approaches Applied To Measurement Of Dietary Exmentioning

confidence: 99%

Measurement of dietary exposure: a challenging problem which may be overcome thanks to metabolomics?

et al. 2009

View full text Add to dashboard Cite

The diet is an important environmental exposure, and its measurement is an essential component of much health-related research. However, conventional tools for measuring dietary exposure have significant limitations being subject to an unknown degree of misreporting and dependent upon food composition tables to allow estimation of intakes of energy, nutrients and non-nutrient food constituents. In addition, such tools may be inappropriate for use with certain groups of people. As an alternative approach, the recent techniques of metabolite profiling or fingerprinting, which allows simultaneous monitoring of multiple and dynamic components of biological fluids, may provide metabolic signals indicative of food intake. Samples can be analysed through numerous analytical platforms, followed by multivariate data analysis. In humans, metabolomics has been applied successfully in pharmacology, toxicology and medical screening, but nutritional metabolomics is still in its infancy. Biomarkers of a small number of specific foods and nutrients have been developed successfully but less targeted and more high-throughput methods, that do not need prior knowledge of which signals might be discriminatory, and which may allow a more global characterisation of dietary intake, remain to be tested. A proof a principle project (the MEDE Study) is currently underway in our laboratories to test the hypothesis that high-throughput, non-targeted metabolite fingerprinting using flow injection electrospray mass spectrometry can be applied to human biofluids (blood and urine) to characterise dietary exposure in humans.

show abstract

Section: Metabolomics Approaches Applied To Measurement Of Dietary Exmentioning

confidence: 99%

Measurement of dietary exposure: a challenging problem which may be overcome thanks to metabolomics?

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Differences in diet can affect the metabolites identified in body fluids. [59][60][61][62] Salicyluric and salicylic acids are examples of compounds that are excreted in urine in higher concentrations in vegetarians than in omnivores. 59) Several of the compounds we have identified in fecal water could be of plant origin.…”

Section: )mentioning

confidence: 99%

NMR Metabolomic Analysis of Fecal Water from Subjects on a Vegetarian Diet

Pettersson

Karlsson

Choi

et al. 2008

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Plant based food contains a multiplicity of bioactive phytochemicals. These compounds are believed to contribute to the cancer protective effect of a diet high in fruit and vegetables.1,2) One group of phytochemicals is the cyclooxygenase-2 (COX-2) inhibitors, which potentially can act as preventive agents against colon cancer. Such compounds and their derivatives may pass through the digestive system and reach the colon where they exert chemopreventive effects.The water fraction of feces (fecal water) is believed to be in direct contact with the colonocytes, and its composition appears to be important for a healthy environment in the colon.3) It is believed that the fecal water composition roughly reflects the food intake and that it contains a mixture of compounds that may prevent and promote cancer development. A favorable change in the composition of fecal water would consequently decrease the risk of developing colon cancer.4) Fecal water is nowadays a common tool used in human intervention studies. It is used to test cellular responses in vitro in cell systems. [5][6][7][8] Such studies have shown that fecal water can modulate several cellular parameters such as genotoxicity, proliferation and apoptosis, all of which are relevant for tumorigenesis. [9][10][11] Other human intervention studies have indicated that it is possible to alter the pH and the composition of bile acids in fecal water with a change in the diet. 3,12) Another parameter that can be modulated by fecal water is the colonic COX-2 enzyme. We have recently shown that fecal waters from human volunteers inhibit COX-2 protein expression and prostaglandin E 2 (PGE 2 ) production in colonic cells. 13)COX-2 is the inducible form of two isoforms of cyclooxygenases, and it catalyzes the production of prostaglandins. This enzyme is not normally expressed in the colon, but it can be induced by growth factors, cytokines, tumor necrosis factor and lipopolysaccharides in situations of stress. [14][15][16] An over expression of COX-2 protein and an increased production of prostaglandins have been reported in patients with colon cancer, [17][18][19] and inhibition of the COX-2 enzyme has been a drug target over the past fifteen years. Today, there are several specific COX-2 inhibitors available on the market, but some of these synthetic drugs were recently reported to have severe side effects on the cardiovascular system. 20,21) To characterize a complex mixture of metabolites such as fecal water, a comprehensive metabolite profiling method is required. Metabolomic profiling of complex extracts is not an easy task to perform since they usually display a wide range of structural diversity. Of the possible analytical methods used in metabolomics, NMR provides a rapid, reproducible method well suited for identification of many metabolites and it has several advantages compared to other methods. It is not only the high information content of the resulting spectra for chemical structures but also the convenience in practical aspects such as high reproducibilit...

show abstract

“…Metabonomics has become a well established analytical tool with successful applications in different fields, e.g., the study of disease processes and drug toxicity (15)(16)(17)(18), and detection of metabolites of inborn errors (19). Recent technological advances have enabled analysis of more subtle metabolic responses to challenges such as nutritional intervention (20), variation in hormonal cycles (21), gender differences, and diurnal variation (22). Moreover, this technology has the ability to differentiate phenotypes of experimental animal models (22).…”

mentioning

confidence: 99%

Metabonomic investigations in mice infected with Schistosoma mansoni : An approach for biomarker identification

Wang

Holmes

Nicholson

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

276

289

View full text Add to dashboard Cite

asis. Using a Schistosoma mansoni-mouse model, we present a characterization of a parasitic infection by metabolic profiling, employing 1 H NMR spectroscopy and multivariate pattern recognition techniques. We infected 10 mice with 80 S. mansoni cercariae each and collected urine samples 49 and 56 days postinfection. Urine samples were also obtained from 10 uninfected control mice at the same time. The metabolic signature of an S. mansoni infection consists of reduced levels of the tricarboxylic acid cycle intermediates, including citrate, succinate, and 2-oxoglutarate, and increased levels of pyruvate, suggesting stimulated glycolysis. A disturbance of amino acid metabolism was also associated with an S. mansoni infection, as indicated by depletion of taurine, 2-oxoisocaproate, and 2-oxoisovalerate and elevation of tryptophan in the urine. A range of microbial-related metabolites, i.e., trimethylamine, phenylacetylglycine, acetate, p-cresol glucuronide, butyrate, propionate, and hippurate, were also coupled with an S. mansoni infection, indicating disturbances in the gut microbiota. Our work highlights the potential of metabolic profiling to enhance our understanding of biological responses to parasitic infections. It also holds promise as a basis for novel diagnostic tests with high sensitivity and specificity and for improved disease surveillance and control.

show abstract

Application of biofluid 1H nuclear magnetic resonance-based metabonomic techniques for the analysis of the biochemical effects of dietary isoflavones on human plasma profile

Cited by 187 publications

References 16 publications

Measurement of dietary exposure: a challenging problem which may be overcome thanks to metabolomics?

Measurement of dietary exposure: a challenging problem which may be overcome thanks to metabolomics?

NMR Metabolomic Analysis of Fecal Water from Subjects on a Vegetarian Diet

Metabonomic investigations in mice infected with Schistosoma mansoni : An approach for biomarker identification

Contact Info

Product

Resources

About