Application of Bio-Inspired Gold Nanoparticles as Advanced Nanomaterial in Halt Nociceptive Pathway and Hepatotoxicity via Triggering Antioxidation System

Abstract

This study aimed to investigate the pharmacological efficacy of gold nanoparticles (GNs) synthesized using a green route, employing the rhizome extract of Euphorbia wallichii (REEW) as a bioreductant and surface stabilizing agent. The GNs were characterized by a series of complementary analytical techniques including SEM-EDX, XRD, DLS, and IR spectroscopy. The reducing ability of REEW and synthesis of GNs were confirmed using UV–visible absorption spectroscopy, and the presence of spherical GNs with an fcc geometry was further confirmed through SEM and XRD analyses. The role of REEW’s extract as a stabilizing agent towards GNs was verified through FTIR and electronic absorption spectral measurement. The GC-MS analysis showed the presence of 41 different phytochemicals in REEW (chloromethyl 2-chloroundecanoate; cortisone; benzo[h]quinolone; piperidine, 2,4-dimethyl- 3,6,7-trimethoxyphenanthroindolizidine; 4-methyl-; 2-[2-quinolylmethyleneamino]ethanol, etc.), with RT values ranging from 3.10 to 27.22 min. The REEW-functionalized GNs exhibited promising antioxidant efficacy against H2O2 and *OH used as probe molecules. DPPH scavenging test showed significant EC50 values of 19.47 µg/mL, 13.53 µg/mL, and 10.57 µg/mL at 30, 60, and 90 min of incubation, respectively. Thermal nociceptive mice significantly acquired analgesia in a dose-dependent manner. Moreover, pre-treatment with REEW-GNs significantly restored serum ALT, AST, ALT, T.P, and Bilirubin levels in PCM-intoxicated mice. The antidiabetic activity in alloxan-induced diabetic rabbits fell in orders of metformin > GNs 300 mg/kg b.w > GNs 200 mg/kg b.w > GNs 100 mg/kg b.w > saline at 0.1 h of drug administration. To sum up, REEW functionalized GNs have tremendous potential for curing degenerative/metabolic diseases caused as a result of oxidative stresses.

Kinetin Capped Zinc Oxide Nanoparticles Improve Plant Growth and Ameliorate Resistivity to Polyethylene Glycol (PEG)-Induced Drought Stress in Vigna radiata (L.) R. Wilczek (Mung Bean)

“…The reaction mixture was placed for one h in the dark and the OD was determined at 517 nm following the protocol of Singleton and Rossi [ 62 ]. The hydrogen peroxide (H 2 O 2 ) scavenging potential was evaluated using the method of Velikova et al [ 63 ], followed by [ 64 , 65 , 66 ]. For this purpose, 0.1 g of fresh mung bean leaves were homogenized in 0.1% TCA and centrifuged for 15 min at 8000 rpm.…”

Production of some Secondary Metabolites of Antibiotic Nature from Mycorrhizal Helper Bacteria (MHB) Associated with Conifers

“…Moreover, FTIR was also used to detect impurities and contaminants in the sample. This method is based on the measurement of wavelength transmission in the range of 500-3500 cm [37][38][39].…”

Evaluation of Ethno-Veterinary Grasses from Suburbs through Microscopic Technique: Insights into Antioxidant Defense System and Phenol

“…ROS are also called free radical oxygen species which include non-radical species such as hydrogen peroxide [23,24]. They are exceptionally susceptible substance species that are released during typical oxygen consuming cellular digestion in any tissue and damage the various intracellular segments, for example, nucleic acids, lipids and proteins [25][26][27][28]. Free radicals generated by endogenous metabolic reactions are associated with various infections such as tumors, shock, irritation, diabetes, cerebrum damage and malignant growth [29,30].…”