Application of bead array technology to simultaneous detection of human leucocyte antigen and human platelet antigen antibodies

Abstract

In this study, we developed a rapid, simple and reliable method for the simultaneous analysis of HLA and HPA antibodies. ICFA can also be used as an alternative to the lymphocyte cytotoxicity test for HLA cross-matching.

The impact of anti-HLA antibodies on unrelated cord blood transplantations

“…For such an occurrence, instead of opting not to use CB for the transplantation, it would be beneficial for transplant centers to have access to confirmative tests for antibodies. A test method that targets antigens naturally expressed on the cell surface, such as immunocomplex capture fluorescence analysis 30 or a lymphocyte indirect immunofluorescence test, 31 or alternative crossmatch tests with HLA-typed cells could be helpful in clinical decision making.…”

Development of a single-antigen magnetic bead assay (SAMBA) for the sensitive detection of HPA-1a alloantibodies using tag-engineered recombinant soluble β3 integrin

“…HPA-1a alloantibodies are responsible for various syndromes in immune-mediated thrombocytopenia. Although a wide range of assays and techniques have been established (Stafford et al, 2008;von dem Borne et al, 1978;Campbell et al, 2007;Meyer et al, 2006a,b;Bakchoul et al, 2007;Fujiwara et al, 2009;Wautier, 2000;Thibaut et al, 2012;Chong et al, 2011;Quiel et al, 2012), the MAIPA is still considered the gold standard for the detection of HPA-1a alloantibodies. However, the sensitivity of many of these assays has been questioned , and interlaboratory variation has been observed in the detection of HPA-specific alloantibodies Sachs et al, 2012).…”

“…The odyssey towards HPA-1a alloantibody detection based on immobilization of platelet antigens with monoclonal antibodies (MoAbs) in the assay known as monoclonal antibody-specific immobilization of platelet antigens assay (MAIPA) was first described by Kiefel et al (1987). Since then, dramatic efforts and progress have been made towards establishing alternative assays for HPA-1a alloantibody detection (Stafford et al, 2008;von dem Borne et al, 1978;Campbell et al, 2007;Meyer et al, 2006a,b;Bakchoul et al, 2007;Fujiwara et al, 2009;Wautier, 2000;Thibaut et al, 2012;Chong et al, 2011;Quiel et al, 2012). However, such assays are hampered by the limited availability of MoAbs and suitable platelet donors.…”

Antibody microarrays for high-throughput, multianalyte analysis

“…Typically, without any detectable assay cross-reactivity or interference, multiple proteins can be simultaneously quantified within a single sample. It has been widely reported that the ELISA microarray is an efficient tool for measuring the levels of up to 50 proteins with excellent sensitive (∼1 pg/ml), dynamic range (∼3 logs), and low coefficients of varia- [93][94][95][96][97][98][99][100][101] Nitrocellulose on a chip Bind to antibodies in a noncovalent, irreversible manner…”

“…Thus, the amount of the targeted PTM on the captured antigens will be measured by this approach. (Colours are visible in the online version of the article; http://dx.doi.org/10.3233/CBM-2009-0140) tion between replicate measurements (5-20%) in small sample volumes (5-20 µl) [13][14][15][16][17][18][19][20][21].…”