Application of Bacteriophages in Nanotechnology

Paczesny, Jan; Bielec, Krzysztof

doi:10.3390/nano10101944

Cited by 42 publications

(19 citation statements)

References 215 publications

(242 reference statements)

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“…Nanoscale materials based on self-assembly of proteins are used for various purposes, including the formation of structurally different shapes; the development of biosensors; the manufacture of optical, conductive, semiconductor, and magnetic nanoelectronics materials; as well as in gene and drug-delivery devices and vaccines [1][2][3]. Viruses, as a natural source of countless self-assembling proteins, are particularly widely studied and adapted for these purposes [4,5]. They come in a wide variety of shapes and sizes.…”

Section: Introductionmentioning

confidence: 99%

Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein

et al. 2021

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We report on the construction of functionalized nanotubes based on tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated 041 protein (041Δ200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. The generated chimeric proteins were successfully synthesized in E. coli BL21 (DE3) cells and self-assembled into tubular structures. We detected the fluorescence of the structures, which was confirmed by stimulated emission depletion microscopy. When 041Δ200GFP and 041Δ200mCherry were coexpressed in E. coli BL21 (DE3) cells, the formed nanotubes generated Förster resonance energy transfer, indicating that both fluorescent proteins assemble into a single nanotube. Chimeric 041Δ200YqfB nanotubes possessed an enzymatic activity, which was confirmed by hydrolysis of N4-acetyl-2′-deoxycytidine. The enzymatic properties of 041Δ200YqfB were similar to those of a free wild-type YqfB. Hence, we conclude that 041-based chimeric nanotubes have the potential for the development of delivery vehicles and targeted imaging and are applicable as scaffolds for biocatalysts.

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Section: Introductionmentioning

confidence: 99%

Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein

et al. 2021

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“…Phage-based plaque detection has the advantage of high sensitivity, but it is time consuming. With the advent of chemical biology, a type of organic–inorganic hybrid probe formed by phage coupling to nanomaterials has been developed, based on the chemical modification of phages and the assembly of metal nanoparticles with the modified phages [ 39 ]. The optical properties of nanomaterials can be used for rapid and sensitive detection.…”

Section: Signals From Nanomaterials Conjugated With Phagesmentioning

confidence: 99%

Advances in the Development of Phage-Based Probes for Detection of Bio-Species

et al. 2022

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Bacteriophages, abbreviated as “phages”, have been developed as emerging nanoprobes for the detection of a wide variety of biological species, such as biomarker molecules and pathogens. Nanosized phages can display a certain length of exogenous peptides of arbitrary sequence or single-chain variable fragments (scFv) of antibodies that specifically bind to the targets of interest, such as animal cells, bacteria, viruses, and protein molecules. Metal nanoparticles generally have unique plasmon resonance effects. Metal nanoparticles such as gold, silver, and magnetism are widely used in the field of visual detection. A phage can be assembled with metal nanoparticles to form an organic–inorganic hybrid probe due to its nanometer-scale size and excellent modifiability. Due to the unique plasmon resonance effect of this composite probe, this technology can be used to visually detect objects of interest under a dark-field microscope. In summary, this review summarizes the recent advances in the development of phage-based probes for ultra-sensitive detection of various bio-species, outlining the advantages and limitations of detection technology of phage-based assays, and highlighting the commonly used editing technologies of phage genomes such as homologous recombination and clustered regularly interspaced palindromic repeats/CRISPR-associated proteins system (CRISPR-Cas). Finally, we discuss the possible scenarios for clinical application of phage-probe-based detection methods.

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“…There are many other studies on this use of phages beyond the scope of this brief overview that are can be found in several recent review articles [ 129 , 134 , 142 , 143 ].…”

Section: Genetically Engineered Phages To Facilitate Tissue Constructionmentioning

confidence: 99%

The Many Applications of Engineered Bacteriophages—An Overview

2021

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Since their independent discovery by Frederick Twort in 1915 and Felix d’Herelle in 1917, bacteriophages have captured the attention of scientists for more than a century. They are the most abundant organisms on the planet, often outnumbering their bacterial hosts by tenfold in a given environment, and they constitute a vast reservoir of unexplored genetic information. The increased prevalence of antibiotic resistant pathogens has renewed interest in the use of naturally obtained phages to combat bacterial infections, aka phage therapy. The development of tools to modify phages, genetically or chemically, combined with their structural flexibility, cargo capacity, ease of propagation, and overall safety in humans has opened the door to a myriad of applications. This review article will introduce readers to many of the varied and ingenious ways in which researchers are modifying phages to move them well beyond their innate ability to target and kill bacteria.

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Application of Bacteriophages in Nanotechnology

Cited by 42 publications

References 215 publications

Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein

Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein

Advances in the Development of Phage-Based Probes for Detection of Bio-Species

The Many Applications of Engineered Bacteriophages—An Overview

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