Application of Bacillus mucilaginosus in the carbonation of steel slag

Jin, Peng; Zhang, Siyi; Liu, Yu; Zhang, Wei; Wang, Ruixing

doi:10.1007/s00253-021-11641-z

Cited by 14 publications

(4 citation statements)

References 40 publications

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“…Although this work was carried out in the context of generating cementitious material, this technology could also be developed for use in ground improvement to build on previous work which found that native Bacillus cereus producing CA could improve sand curing [ 51 ]. CA has also been shown to be effective in bioremediation of steel slag [ 52 ], and utilisation of these novel strains in conjunction with S. pasteurii could be used in the same way.…”

Section: Resultsmentioning

confidence: 99%

Microbially induced calcium carbonate precipitation through CO2 sequestration via an engineered Bacillus subtilis

Gilmour,

Ghimire,

Wright

et al. 2024

Microb Cell Fact

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Background Microbially induced calcium carbonate precipitation has been extensively researched for geoengineering applications as well as diverse uses within the built environment. Bacteria play a crucial role in producing calcium carbonate minerals, via enzymes including carbonic anhydrase—an enzyme with the capability to hydrolyse CO2, commonly employed in carbon capture systems. This study describes previously uncharacterised carbonic anhydrase enzyme sequences capable of sequestering CO2 and subsequentially generating CaCO3 biominerals and suggests a route to produce carbon negative cementitious materials for the construction industry. Results Here, Bacillus subtilis was engineered to recombinantly express previously uncharacterised carbonic anhydrase enzymes from Bacillus megaterium and used as a whole cell catalyst allowing this novel bacterium to sequester CO2 and convert it to calcium carbonate. A significant decrease in CO2 was observed from 3800 PPM to 820 PPM upon induction of carbonic anhydrase and minerals recovered from these experiments were identified as calcite and vaterite using X-ray diffraction. Further experiments mixed the use of this enzyme (as a cell free extract) with Sporosarcina pasteurii to increase mineral production whilst maintaining a comparable level of CO2 sequestration. Conclusion Recombinantly produced carbonic anhydrase successfully sequestered CO2 and converted it into calcium carbonate minerals using an engineered microbial system. Through this approach, a process to manufacture cementitious materials with carbon sequestration ability could be developed.

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Section: Resultsmentioning

confidence: 99%

Microbially induced calcium carbonate precipitation through CO2 sequestration via an engineered Bacillus subtilis

Gilmour,

Ghimire,

Wright

et al. 2024

Microb Cell Fact

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“…Biological tools have also been used to enhance carbonation. Jin et al accelerated calcium carbonate precipitation by employing Bacillus mucilaginosus on steel slag powder [ 197 ], increasing the carbonation degree from 66.34 to 86.25%. Moreover, the mechanical properties and durability of the treated steel slag were enhanced.…”

Section: Carbonic Anhydrase In Carbon Capture Storagementioning

confidence: 99%

“…At a dosage of bacterial powders of 1 wt.%, carbonation reached the maximum. [197] To develop and characterize a highly efficient and stable biocatalyst for CO 2 sequestration using silica nanocomposite with auto-encapsulated CA. (CA)-based biocatalyst encapsulated in a biosilica with a peptide R5.…”

Section: Chemical Absorptionmentioning

confidence: 99%

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

et al. 2023

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Direct biocatalytic processes for CO2 capture and transformation in value-added chemicals may be considered a useful tool for reducing the concentration of this greenhouse gas in the atmosphere. Among the other enzymes, carbonic anhydrase (CA) and formate dehydrogenase (FDH) are two key biocatalysts suitable for this challenge, facilitating the uptake of carbon dioxide from the atmosphere in complementary ways. Carbonic anhydrases accelerate CO2 uptake by promoting its solubility in water in the form of hydrogen carbonate as the first step in converting the gas into a species widely used in carbon capture storage and its utilization processes (CCSU), particularly in carbonation and mineralization methods. On the other hand, formate dehydrogenases represent the biocatalytic machinery evolved by certain organisms to convert CO2 into enriched, reduced, and easily transportable hydrogen species, such as formic acid, via enzymatic cascade systems that obtain energy from chemical species, electrochemical sources, or light. Formic acid is the basis for fixing C1-carbon species to other, more reduced molecules. In this review, the state-of-the-art of both methods of CO2 uptake is assessed, highlighting the biotechnological approaches that have been developed using both enzymes.

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“…The carbonates formed can store carbon permanently in the ground and potentially reduce the amount of CO 2 accumulation in the atmosphere [6]. Biocementation can be realised by different pathways, such as urea hydrolysis [7], denitrification [8], carbonic anhydrase [9] and methane oxidation [10] amongst others, or by a combination of processes [11].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Utilisation of Hybrid Metal-Carbonic Anhydrase Enzyme Carrier System for Soil Biocementation

Mwandira,

Purchase,

Mavroulidou

et al. 2023

Applied Sciences

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Biocementation is an emerging nature-inspired method of producing eco-friendly cement for soil stabilization. This paper used the bovine-derived carbonic anhydrase (CA) enzyme to catalyse the bioprecipitation of CaCO3 in a fine-grained soil and thus to biocement the soil. To increase the efficiency of the CA, an innovative copper–carbonic anhydrase (CA) hybrid was fabricated. This study is a proof-of-concept of the potential application of these enzyme carriers for soil biocementation. The hybrid carriers are aimed to enhance the stability, recovery and reusability of the enzyme used in the biocementation process. The results showed that the fabricated copper phosphate-based inorganic hybrid was stable throughout the duration of the tests (2 months) and under a wide range of pH and temperatures. Its enzymatic activity was enhanced compared to the free CA enzyme and it was proved suitable for soil biocementation. This was further confirmed by the SEM analysis. Additionally, the treated soil with the formulated hybrid carrier showed improved unconfined compressive strength, especially when the carriers were implemented into the soil by mixing. The material analysis by Raman spectroscopy confirmed calcium carbonate as the primary precipitate, consistent with soil biocementation. Overall, this innovative method of delivery of enzymes with enhanced stability and activity shows promise that, upon further development, it can be successfully used to increase the efficiency and sustainability of the biocementation process.

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Application of Bacillus mucilaginosus in the carbonation of steel slag

Cited by 14 publications

References 40 publications

Microbially induced calcium carbonate precipitation through CO2 sequestration via an engineered Bacillus subtilis

Microbially induced calcium carbonate precipitation through CO2 sequestration via an engineered Bacillus subtilis

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

Synthesis and Utilisation of Hybrid Metal-Carbonic Anhydrase Enzyme Carrier System for Soil Biocementation

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