Application of Avidin−Biotin Technology and Adsorptive Transfer Stripping Square-Wave Voltammetry for Detection of DNA Hybridization and Avidin in Transgenic Avidin Maize

Masařík, Michal; Kizek, René; Kramer, Karl J.; Billová, Sabina; Vacek, Jan; Bailey, Murray; Jelen, František; Howard, John A.

doi:10.1021/ac020788z

Cited by 110 publications

(55 citation statements)

References 36 publications

(74 reference statements)

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“…The filtrate was centrifuged at 14 500 g for 15 min at 4°C. The pH of the supernatant was adjusted to pH 10.5 and then was centrifuged at 14 000 g for 30 min (Eppendorf 5402) in 4°C (Hood et al 1997, Masarik et al 2003). The resulting supernatant was then used to prepare the modified CPE.…”

Section: Methodsmentioning

confidence: 99%

“…It is known that the carbon paste electrode (CPE) is suitable for biosensors construction (Wang 1985, Wang et al 1997, Wang et al 1999, Wang et al 2000. Moreover, the carbon paste electrode can be modified by addition of various compounds in order to increase the sensitivity, selectivity and rapidness of the determination (Wang and Lin 1988, Kubiak and Wang 1989, Masarik et al 2003, Kizek et al 2005). In our work we used the carbon electrodes (CPE and modified CPE) coupled with square-wave voltammetry to measure nanogram amount of avidin.…”

mentioning

confidence: 99%

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The determination of avidin in genetically modified maize by voltammetric techniques

Petrlová¹,

Křížková²,

Šupálková³

et al. 2007

Plant Soil Environ.

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Quality assurance is a major issue in the food industry. The authenticity of food ingredients and their traceability are required by consumers and authorities. Plant species such as barley (Hordeum vulgare), rice (Oryza sativa), sunflower (Helianthus annus), wheat (Triticum aestivum) and maize (Zea mays) are very common objects of interest of genetic modification (GMO); therefore the development of specific assays for their specific detection and quantification of GMO are needed. Furthermore, the production and trade of genetically modified lines from an increasing number of plant species brings about the need for control within research, environmental risk assessment, labeling-legal, and consumers’ information purposes. Electrochemical sensors and biosensors based on modification of working electrode could be suitable tools for these purposes. Here, we report using of an avidin-modified carbon paste electrode for rapid and sensitive determination of avidin in plant extract solution and in a transgenic maize extract. The process could be used to determine avidin concentrations up to 3pM in solution and 170nM in a maize seed extract. Moreover, we applied the method to analyze different maize flours.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

The determination of avidin in genetically modified maize by voltammetric techniques

Petrlová¹,

Křížková²,

Šupálková³

et al. 2007

Plant Soil Environ.

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“…Electrochemical method for measurement of recombinant protein levels ( Fig. 8) using transgenic avidin maize as a model GMO, was reported by Masarik et al [158]. A disposable genosensor for GMO has been reported by Meric et al through detecting the nearly ubiquitous genetic element of transgenic organisms -NOS-terminator [159].…”

Section: Gmo Contentmentioning

confidence: 99%

“…(14), aprotinin (7.6); 2) native streptavidin; 3) thermally denatured streptavidin (99 8C, 30 min); 4) 10 mg of avidin-positive maize seed extract; 5) 10 mg of avidin-negative maize seed extract (negative control); 6) 10 mg of avidin-negative corn low-grade flour extract; 7) 10 mg of avidin-negative corn grout extract; 8) 10 mg of avidin-negative corn grits extract, and 9) 10 mg of avidin-negative corn farina extract. (from [158] with permission).…”

Section: Acknowledgementsmentioning

confidence: 99%

Electrochemical Biosensors for Medical and Food Applications

2008

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Sensing and quantification of DNA and proteins are becoming increasingly important in biochemistry, medicine and biotechnology. Traditional analytical techniques are lagging behind the demand for more information in less time, at a lower cost. An important step forward in this pursuit is to identify an analyte using its electrochemical behavior and to convert its presence and concentration into perceivable and distinct electrical signals. This review covers the strategies for electrochemical sensing of biomolecules, mainly, DNA and proteins by label-based and label-free approaches using disposable electrochemical printed chips and carbon nanotube based field effect transistors. Issues, such as ease of preparation, robustness, sensitivity, and realization of mass production of the detection strategies are also considered. A good coverage of the published literature, mostly, a wide treatment of original research articles reporting novel principles has been made. Finally, this review may help the researchers in developing an understanding of miniaturized electrochemical biosensors and their possible applications in medical and food science, with directions for future research.

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“…Under this scope, a series of experiments were performed using concentration levels for STR and bio-ODN of 2000 mg mL À1 and 100 mg mL À1 , respectively. In the case of STR modified CNT-PGEs, the voltammogram showed a broad peak at potential of þ 0.798 V (shown in Figure 1Ab), which is attributed to tryptophan and tyrosine residues in STR that can be oxidized electrochemically at carbon electrodes [29,30]. The biotinylated DNA attachment onto STR coated surface caused a decrease at the signal of STR ( Figure 1Ac) as a result of the specific and strong binding between STR and biotin.…”

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confidence: 98%

Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label‐Free Sequence Specific DNA Detection

et al. 2010

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Disposable graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (MWCNTs)-streptavidin (STR) conjugates were used for electrochemical monitoring of label-free DNA hybridization. The surface morphology of PGE electrode before and after hybridization was characterized by scanning electron microscopy. Electrochemical impedance spectroscopy was used to monitor each step of the construction of the DNA biosensor. The biosensor was demonstrated to have excellent selectivity, being able to differentiate complementary sequences from a noncomplementary ones and in addition select the target sequence of DNA from a mixture of other DNA without loss in current sensitivity.

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Application of Avidin−Biotin Technology and Adsorptive Transfer Stripping Square-Wave Voltammetry for Detection of DNA Hybridization and Avidin in Transgenic Avidin Maize

Cited by 110 publications

References 36 publications

The determination of avidin in genetically modified maize by voltammetric techniques

The determination of avidin in genetically modified maize by voltammetric techniques

Electrochemical Biosensors for Medical and Food Applications

Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label‐Free Sequence Specific DNA Detection

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