Application of autologous bone marrow mononuclear cells in six patients with advanced chronic critical limb ischemia as a result of diabetes: our experience

Subrammaniyan, R; Amalorpavanathan, J; Shankar, R; Rajkumar, M; Baskar, Sivasubramanian; Manjunath, S; Senthilkumar, Rajappa; Murugan, P; Srinivasan, V.; Abraham, S

doi:10.3109/14653249.2011.579961

Cited by 28 publications

(17 citation statements)

References 34 publications

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“…Recently, tissue resident stem cells from human retinal pigment epithelium were grown on a polyester matrix and transplanted into rats with age-related macular degeneration with success [10]. Of particular relevance, CD34+ stem cells have been associated with enhanced healing, secretion of anti-inflammatory molecules, and revascularization of injured tissue [11–14]. These attributes recommend CD34+ cells as strong candidates for cellularized biomaterials for placement along vascular grafts, and CD34+ cells are assessed in the present work.…”

Section: Introductionmentioning

confidence: 99%

Decreasing matrix modulus of PEG hydrogels induces a vascular phenotype in human cord blood stem cells

et al. 2015

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Adult and congenital cardiovascular diseases are significant health problems that are often managed using surgery. Bypass grafting is a principal therapy, but grafts fail at high rates due to hyperplasia, fibrosis, and atherosclerosis. Biocompatible, cellularized materials that attenuate these complications and encourage healthy microvascularization could reduce graft failure, but an improved understanding of biomaterial effects on human stem cells is needed to reach clinical utility. Our group investigates stem-cell-loaded biomaterials for placement along the adventitia of at-risk vessels and grafts. Here, the effects of substrate modulus on human CD34+ stem cells from umbilical cord blood were evaluated. Cells were isolated by immunomagnetic separation and encapsulated in 3, 4, and 6 weight% PEG hydrogels containing 0.032% gelatin and 0.0044% fibronectin. Gels reached moduli of 0.34, 4.5, and 9.1 kPa. Cell viability approached 100%. Cell morphologies appeared similar across gels, but proliferation was significantly lower in 6 wt% gels. Expression profiling using stem cell signaling arrays indicated enhanced self-renewal and differentiation into vascular endothelium among cells in the lower weight percent gels. Thus, modulus was associated with cell proliferation and function. Gels with moduli in the low kilopascal range may be useful in stimulating cell engraftment and microvascularization of graft adventitia.

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Section: Introductionmentioning

confidence: 99%

Decreasing matrix modulus of PEG hydrogels induces a vascular phenotype in human cord blood stem cells

et al. 2015

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“…It has been reported that MSCs may also be used for treatment of ischemic diseases (15–18). In a study by Subrammaniyan et al (19), injection of bone marrow-derived mononuclear cells had satisfactory efficacy in the treatment of six cases of DM with critical limb ischemia and skin defects, and amputation was avoided in all patients undergoing autologous bone marrow-derived mononuclear cell injection.…”

Section: Discussionmentioning

confidence: 97%

Autologous bone marrow stem cell transplantation for the treatment of postoperative hand infection with a skin defect in diabetes mellitus: A case report

Liu

Wang

et al. 2014

Oncology Letters

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Among stem cells, autologous mesenchymal stem cells (MSCs) are ideal for transplantation by virtue of limited rejection reactions and marked proliferative ability. This study presents a novel method by which MSCs were harvested from the bone marrow of a patient who presented with severe post-traumatic infection and a non-healing skin defect in the hand, secondary to uncontrolled diabetes mellitus (DM). An autologous MSC suspension was injected into the persistent skin defect after stabilizing the blood glucose level and appropriate infection control. During the course of a regular 18-month postoperative follow-up, the patient exhibited immediate recovery with no transplant-associated complications, as well as no evidence of tumorigenicity. Thus, transplantation of autologous MSCs may play a role in the clinical application of stem cells, particularly for treatment of skin defects following surgery in cases of DM and for those caused by various other traumas.

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“…Peripheral blood stem cells are mainly selected by their CD34 antigen positivity. Different preparations of the hematopoietic cells have been claimed to be effective in correcting hyperglycaemia, improvement of endogenous insulin production, and diminishing or eliminating the need for insulin and other diabetes controlling treatments [19][20][21]. Wang, et al, [22] used autologous bone marrow to treat 31 patients with stem cell infusion into the major 7) Stem cell research also benefits the study of development stages that cannot be studied directly in a human embryo, which sometimes are linked with major clinical consequences such as birth defects, pregnancy-loss and infertility.…”

Section: Hematopoietic and Bone Marrow Cells For Type 2 Diabetes Mellmentioning

confidence: 99%

Stem Cell Therapy: Recent Success and Continuing Progress in Treating Diabetes

Mathias¹,

Goveas²,

Manish³

2018

Int J Stem Cell Res Ther

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Application of autologous bone marrow mononuclear cells in six patients with advanced chronic critical limb ischemia as a result of diabetes: our experience

Cited by 28 publications

References 34 publications

Decreasing matrix modulus of PEG hydrogels induces a vascular phenotype in human cord blood stem cells

Decreasing matrix modulus of PEG hydrogels induces a vascular phenotype in human cord blood stem cells

Autologous bone marrow stem cell transplantation for the treatment of postoperative hand infection with a skin defect in diabetes mellitus: A case report

Stem Cell Therapy: Recent Success and Continuing Progress in Treating Diabetes

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