Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding

Abstract

Atmospheric and room-temperature plasma (ARTP) is an efficient microbial mutagenesis method with broad application prospects. Compared to traditional methods, ARTP technology can more effectively induce DNA damage and generate stable mutant strains. It is characterized by its simplicity, cost-effectiveness, and avoidance of hazardous chemicals, presenting a vast potential for application. The ARTP technology is widely used in bacterial, fungal, and microalgal mutagenesis for increasing productivity and improving characteristics. In conclusion, ARTP technology holds significant promise in the field of microbial breeding. Through ARTP technology, we can create mutant strains with specific genetic traits and improved performance, thereby increasing yield, improving quality, and meeting market demands. The field of microbial breeding will witness further innovation and progress with continuous refinement and optimization of ARTP technology.

Comprehensive Analysis of Catalytic Characteristics and Molecular Mechanisms in Mutant Trametes versicolor Strains with Enhanced Laccase Activities

“…The spore mortality rate gradually increased as the ARTP treatment time was extended, with a gradient of 10 s. It reached 92.330% at 50 s and further rose to 97.640% at 60 s. Notably, by the end of the treatment period (90 s), there was virtually no survival observed among the original strain (Figure 1a). According to previous reports, a lethality rate of 90% is considered appropriate [31,32]. Therefore, in this experiment, an exposure time of 50 s was selected to achieve the desired lethality rate.…”

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

“…The mortality rate of the strain increases with the treatment time. In general, the optimal treatment time is chosen to bring the strain mortality rate to about 90%, since the probability of obtaining a positive mutant strain is relatively high at this time 12,31 . The second part is strain growth stage and strain concentration.…”

“…The growth stage of the strain is also considered to be an important influencing factor. Since strains in the logarithmic growth stage can maintain a relatively large rate of growth and reproduction, they are more sensitive to the effects of external factors 12 . Therefore, strains at this stage were selected for mutagenesis.…”

“…The third part is the subsequent dilution and screening method. Individual dispersed microbial colonies were obtained by uniformly streaking the treated microbial cells onto agar plates 12 . Finally, ARTP, as a random mutagenesis method, requires a targeted selection of colony screening methods according to the specific research objectives 33 .…”

“…The working atmosphere of ARTP is mainly He or Ar, which facilitates the generation of a wide range of active species by plasma excitation, and thus facilitates the stimulation of different biological effects, such as biopathways, DNA damage, and DNA repair 11 . Compared with traditional random mutagenesis, ARTP produces higher DNA damage capacity (e.g., acting more efficiently on genes or genomes, and also causing alterations in multiple genes at the same time) with its unique properties, resulting in better mutation effects 12 . In addition, it has the advantages of simple operation process, mild experimental conditions, high compatibility with conventional mutagenesis, and fast mutation speed.…”