Application of Aqueous Two-Phase Systems for the Recovery of Bioactive Low-Molecular Weight Compounds

Simental-Martínez, Jesús; Montalvo-Hernández, Bertha; Rito‐Palomares, Marco; Benavides, Jorge

doi:10.1080/01496395.2014.904878

Cited by 21 publications

(24 citation statements)

References 34 publications

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“…As shown in Figure 1, for the ethanol /(NH 4 ) 2 SO 4 ATPS, the highest K was attained for the highest TLL (57% w/w, system 3). This behaviour may be related with the hydrophobicity of both phases (Simental-Martínez et al 2014). An increase in TLL resulted in an increase of ethanol and salt concentrations on the top and bottom phases, respectively.…”

Section: Effect Of the Tie Lie Length On The Partition Behaviour Of Pmentioning

confidence: 99%

“…An increase in TLL resulted in an increase of ethanol and salt concentrations on the top and bottom phases, respectively. Thus, an increase in TLL resulted in a major difference among both phases, which led to increase the hydrophobicity of the ethanol phase favoring partitioning (Lucena de Souza et al 2010, Simental-Martínez et al 2014.…”

Section: Effect Of the Tie Lie Length On The Partition Behaviour Of Pmentioning

confidence: 99%

“…Many researchers have suggested that TLL, which relates the mass ratio between phases in equilibrium, could have influence on product recovery (Simental-Martínez et al 2014). In order to examine the behaviour of phenol recovery, three different tie lie lengths (TLL) were selected for each ATPS at a V r value around 1,0 to avoid any potential concentration effect (Table 1).…”

Section: Effect Of the Tie Lie Length On The Partition Behaviour Of Pmentioning

confidence: 99%

“…Moreover, alcohol-salt ATPS are of low viscosity, and usually led to high extraction efficiencies and purification levels in a single-step procedure (Reis et al 2014). The low viscosity is industrially relevant as the cost of pumping fluids is reduced (Simental-Martínez et al 2014). Alcohol-salt systems have received less attention because many biomolecules (such as proteins and enzymes) are incompatible with organic solvent phase, which can inactivate or denature the biomolecules (Ooi et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…(Rosa et al 2010, Simental-Martínez et al 2014. The most commonly used systems are based on polymer-polymer or polymer-salt.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Recovery of phenolic compounds from Eucalyptus wood wastes using ethanol-salt-based aqueous two-phase systems

Xavier¹,

Freire²,

Vidal³

2017

Maderas, Cienc. tecnol.

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In this work the partition behavior of phenols using ethanol-salt-based aqueous two phase systems (ATPS) was evaluated. The aim was the recovery of phenolic compounds with antioxidant capacity from eucalyptus wood industrial wastes. Experiments were planned to study the influence of several parameters on phenols partition, including type of inorganic salt (ammonium sulphate, a mixture of monopotassium phosphate and potassium diphosphate and potassium diphosphate), tie-lie lenght (TLL), volume ratio (V r ), settlement time and temperature. Phenols could be recovered preferently from the top or bottom phases depending on the salt used. It was demostrated that tie-lie lenght, volume ratio and temperature had influence on phenols partition. The highest total phenols yield 5,36 mg gallic acid equivalent (100 mg oven dried wood) -1 and FRAP antioxidant activity, 20 mmol AAE (100 g oven dried wood) -1 , was obtained using ATPS formed by 40.6% (w/w) ethanol and 12% (w/w) of ammonium sulphate at 65ºC. Analysis of the extract by RP-HPLC-ESI-TOF confirmed the presence of the phenolic compounds with potential antioxidant activity, namely, ellagic acid, myricetin 3-O-rhamnoside and quercetin 3-glucoside.

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Section: Effect Of the Tie Lie Length On The Partition Behaviour Of Pmentioning

confidence: 99%

Section: Effect Of the Tie Lie Length On The Partition Behaviour Of Pmentioning

confidence: 99%

Section: Effect Of the Tie Lie Length On The Partition Behaviour Of Pmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…(Rosa et al 2010, Simental-Martínez et al 2014. The most commonly used systems are based on polymer-polymer or polymer-salt.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Recovery of phenolic compounds from Eucalyptus wood wastes using ethanol-salt-based aqueous two-phase systems

Xavier¹,

Freire²,

Vidal³

2017

Maderas, Cienc. tecnol.

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Primary recovery of bioactive compounds from stressed carrot tissue using aqueous two‐phase systems strategies

Sánchez-Rangel

Jacobo‐Velázquez

Cisneros‐Zevallos

et al. 2014

J of Chemical Tech & Biotech

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BACKGROUND The application of controlled postharvest abiotic stresses in carrots induces the production of phenolic compounds, mainly chlorogenic acids, which present diverse biological functions. Aqueous two‐phase systems (ATPS) is a liquid–liquid extraction technique that has demonstrated its potential for the fractionation and recovery of bioactive compounds. ATPS have the advantages of using environmentally friendly constituents, ease of scale‐up and process integration capability. This experimental work addresses for the first time the recovery of bioactive compounds (mainly hydroxycinnamic acid derivatives) from abiotically stressed carrot tissue using ATPS strategies, including in situ extraction, in alcohol–salt and ionic liquid–salt systems. RESULTS The partitioning of chlorogenic acid was favored in the ethanol–potassium phosphate system achieving a recovery yield of 81.9%. Hydroxycinnamic acid derivatives fractioning was favored in 1‐ethyl‐3‐methylimidazolium acetate (EMIM‐Ac)–potassium phosphate ATPS, where recovery yields up to 85.4% were achieved. The in situ extraction strategy not only demonstrated potential for merging extraction and recovery in the same system but also, depending on ATPS used, allowed differential phenolic profiles to be obtained. CONCLUSION ATPS strategies (either solid–liquid extraction followed by ATPS or in situ extraction) were shown to have great potential for the recovery of bioactives from stressed carrot tissue. © 2014 Society of Chemical Industry

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Recovery of roseoflavin from a recombinant Streptomyces davaonensis strain by using biphasic aqueous systems

Ibarra‐Herrera

Meza-Dorantes

Mora-Lugo

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND Roseoflavin (RoS) is a naturally occurring red color riboflavin analog compound, produced mainly by Streptomyces davaonensis, with a rising profile owing to its antibacterial potential. However, there is a lack of strategies for its downstream processing and the existing ones are limited to chromatographic techniques involving harmful reagents. The use of aqueous two‐phase systems (ATPS), a liquid–liquid extraction method, is explored in this work as an alternative strategy to recover and purify RoS from S. davaonensis cultures. RESULTS Polymer‐phosphate, alcohol‐phosphate and ionic liquid (IL)‐phosphate systems that varied in composition were tested, where polyethylene glycol (PEG), ethanol (EtOH) and 1‐ethyl‐3‐methylimidazolium acetate ([C2mim] [OAC]) represent the top phases, and phosphate the bottom phase. The partition behavior of either RoS alone or with contaminants was evaluated. In both cases, RoS showed a preference for top phase in all the systems tested. When cell‐free cultures were added to the systems, IL‐salt (13% and 30% w/w), EtOH‐phosphate [50% tie‐line length (TLL)] and PEG8000‐phosphate (35% w/w TLL) systems presented the highest RoS recovery (>95%), but separation of RoS and contaminants was not accomplished. The systems that demonstrated separation of RoS and contaminants were the PEG3350‐ and PEG8000‐salt systems, both with a 15% w/w TLL, as RoS and contaminants partitioned to top and bottom phases, respectively. CONCLUSION This is the first attempt at using ATPS to recover RoS from S. davaonensis cultures. It has been proven that RoS can be recovered directly from S. davaonensis cultures using biphasic systems. This strategy may represent a greener alternative to chromatographic techniques. © 2021 Society of Chemical Industry (SCI).

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Application of Aqueous Two-Phase Systems for the Recovery of Bioactive Low-Molecular Weight Compounds

Cited by 21 publications

References 34 publications

Recovery of phenolic compounds from Eucalyptus wood wastes using ethanol-salt-based aqueous two-phase systems

Recovery of phenolic compounds from Eucalyptus wood wastes using ethanol-salt-based aqueous two-phase systems

Primary recovery of bioactive compounds from stressed carrot tissue using aqueous two‐phase systems strategies

Recovery of roseoflavin from a recombinant Streptomyces davaonensis strain by using biphasic aqueous systems

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