Application of Apodization Digital Holography in Phase Measurement

Yancao, 张延曹 Zhang; Jianlin, 赵建林 Zhao; Qi, 范琦 Fan; Sheng, 杨盛 Yang

doi:10.3788/cjl20103706.1602

中国激光

2010

DOI: 10.3788/cjl20103706.1602

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Application of Apodization Digital Holography in Phase Measurement

张延曹 Zhang Yancao¹,

赵建林 Zhao Jianlin²,

范琦 Fan Qi³

et al.

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“…Several automatic cell counters have been developed recently, but all of them require the prestaining of cells by chemical reagents such as trypan blue or propium iodide [4] . Because of these issues, many optical methods have been studied to achieve the visual observation of living cells, such as Fourier phase microscopy (FPM) [5] , Hilbert phase microscopy (HPM) [6] , diffraction phase microscopy (DPM) [7] , and digital holographic microscopy (DHM) [8,9] . Among these, DHM attracts the most remarkable attention for several advantages.…”

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confidence: 99%

基于无透镜数字全息成像的活体细胞培养状态无损监测

Wang¹,

Wang²,

Zhao³

et al. 2011

中国光学快报

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A non-invasive detection method for the status analysis of cell culture is presented based on digital holography technology. Lensless Fourier transform digital holography (LFTDH) configuration is developed for living cell imaging without prestaining. Complex amplitude information is reconstructed by a single inverse fast Fourier transform, and the phase aberration is corrected through the two-step phase subtraction method. The image segmentation is then applied to the automatic evaluation of confluency. Finally, the cervical cancer cell TZMbl is employed for experimental validation, and the results demonstrate that LFTDH imaging with the corresponding image post-processing can provide an automatic and non-invasive approach for monitoring living cell culture.

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mentioning

confidence: 99%

基于无透镜数字全息成像的活体细胞培养状态无损监测

Wang¹,

Wang²,

Zhao³

et al. 2011

中国光学快报

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Improvement of Fresnel Diffraction Convolution Algorithm

Ge,

Song,

Caiyang

et al. 2024

Applied Sciences

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With the development of digital holography, the accuracy requirements for the reconstruction phase are becoming increasingly high. The transfer function of the double fast transform (D-FFT) algorithm is distorted when the diffraction distance is larger than the criterion distance dt, which reduces the accuracy of solving the phase. In this paper, the Fresnel diffraction integration algorithm is improved by using the low-pass Tukey window to obtain more accurate reconstructed phases. The improved algorithm is called the D-FFT (Tukey) algorithm. The D-FFT (Tukey) algorithm adjusts the degree of edge smoothing of the Tukey window, using the peak signal-to-noise ratio (PSNR) and the structural similarity (SSIM) to remove the ringing effect and obtain a more accurate reconstructed phase. In a simulation of USAF1951, the longitudinal resolution of the reconstructed phase obtained by D-FFT (Tukey) reached 1.5 μm, which was lower than the 3 μm obtained by the T-FFT algorithm. The results of Fresnel holography experiments on lung cancer cell slices also demonstrated that the phase quality obtained by the D-FFT (Tukey) algorithm was superior to that of the T-FFT algorithm. D-FFT (Tukey) algorithm has potential applications in phase correction, structured illumination digital holographic microscopy, and microscopic digital holography.

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Application of Apodization Digital Holography in Phase Measurement

Cited by 2 publications

References 0 publications

基于无透镜数字全息成像的活体细胞培养状态无损监测

基于无透镜数字全息成像的活体细胞培养状态无损监测

Improvement of Fresnel Diffraction Convolution Algorithm

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