Application of analytical and preparative high-speed counter-current chromatography for separation of alkaloids from Coptis chinensis Franch

Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC

“…It is known to contain protoberberine alkaloids such as berberine, palmatine, coptisine, epiberberine, jatrorrhizine, columbamine and berberastin. Another TCM Phellodendron, used in clinical treatment some cases in combination with Coptis, is reported as an anti-stomachic, anti-inflammatory and antipyretic agent with the major constituents of protoberberine alkaloids [20,21]. Fig.…”

An Economical Method for Preparative Purification of Five Alkaloids from Coptis Chinensis Franch by High-Speed Counter-Current Chromatography Using Singled Prepared Solvent System by GC

“…Many papers have been reported to separate alkaloids from this plant by HSCCC [22,23]. The solvent system composed of chloroform-methanol-water is considered to be one of the classical solvent systems in alkaloids purification, but the ratios between the solvents were not invariable.…”

“…Yang et al [22] have used an optimum solvent system to separate four alkaloids including palmatine, berberine, epiberberine and coptisine from C. chinensis by preparative HSCCC, successfully. Peng et al [23] also separated four alkaloids with high recoveries from C. chinensis by HSCCC with direct injection the powders of a raw materials without any preparation.…”

Countercurrent Chromatography in Analytical Chemistry (IUPAC Technical Report)

“…CCC was used for the systematic selection and optimization of a two-phase solvent system to separate alkaloids from Coptis chinensis Franch using a system of chloroform-methanol-HCl solution (0.3-0.1 mol/L) at different volume ratios [42]. One separation run yielded four pure alkaloids, including palmatine, berberine, epiberberine, and coptisine from a crude alkaloid extract.…”