Application of an HPLC assay for the determination of folate derivatives in some vegetables, fruits and berries consumed in Finland

Vahteristo, Liisa; Lehikoinen, Kaisa; Ollilainen, Velimatti; Varo, Pertti

doi:10.1016/s0308-8146(96)00318-4

Cited by 110 publications

(97 citation statements)

References 18 publications

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“…The folate levels varied from 30 to 69 mg/100 g of fresh weight (FW), with a mean concentration in all strawberry cultivars of 47 6 9 mg/100 g of FW (Table 1); water content in the fruits varied between 87 % and 93 %. The values obtained appeared to be comparable to the data reported from previously published studies, where contents ranged from 36 (15) to 65 (16) mg/100 g of FW, and to values indicated in European food data tables, where folate in strawberries range from 20 to 99 mg/100 g FW. In 2006, our group started the evaluation of folate content in different strawberry varieties and selections cultivated at the experimental field of Marche Polytechnic University (4) .…”

Section: Strawberry Phytochemicals and Evidence For Biological Efficacysupporting

confidence: 88%

Impact of strawberries on human health: insight into marginally discussed bioactive compounds for the Mediterranean diet

Tulipani

Mezzetti

Battino

2009

Public Health Nutr.

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Objective: To review and update the current knowledge on the potential impact of strawberry on human health, with particular attention on compounds and indirect mechanisms of action not exhaustively considered. Design: Personal perspectives and recent data. Setting: International. Results: Our research group was among the few groups that have recently investigated the folate content in fresh, stored and processed strawberries, and the data look very promising. As well, some in vivo evidence of the impact of strawberry intake on the folate status in humans have already been reported, but a new increasing interest on this field is strongly hoped. Furthermore, the hypouricaemic effects previously ascribed to cherry consumption need to be evaluated in respect to strawberry intake. At the moment, inconsistent results come from the few investigations designed at this proposal. In our studies, a great interindividual variability was observed on plasma urate levels in response to strawberry intake, suggesting a putative effect. Conclusions: The mechanisms responsible for the potential health-promoting effects of strawberry may not be necessarily searched in the activity of phytochemicals. Particularly, a greater interest should be addressed to show whether a prolonged strawberry consumption may effectively improve the folate status and reduce the incidence of folate-related pathological conditions. Furthermore, the hypouricaemic effects of cherries need to be evaluated also in respect to strawberry intake, and the mechanisms of actions and anti-gout potentialities need to be studied in detail.Future investigations involving human trials should be aimed at following these underestimated scientific tracks.

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Section: Strawberry Phytochemicals and Evidence For Biological Efficacysupporting

confidence: 88%

Impact of strawberries on human health: insight into marginally discussed bioactive compounds for the Mediterranean diet

Tulipani

Mezzetti

Battino

2009

Public Health Nutr.

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“…Unlike 5-methyltetrahydrofolate which is the predominant natural form of folate in plants, 74 folic acid has to be reduced to tetrahydrofolate, and then further converted to the active form 5-methyltetrahydrofolate in the body (Figure 1). As shown in Figure 1, the formation of 5,10-methylenetetrahydrofolate from tetrahydrofolate requires a onecarbon unit from serine, dimethylglycine, sarcosine or glycine.…”

Section: Folic Acidmentioning

confidence: 99%

“…20 Vegetables generally contain much more betaine than folate. For example, 100 g of spinach, one of the richest vegetable sources of folate, contains only B50 mg of folate, 74 but has B600 mg of betaine and 22 mg of choline. 77 Therefore, diet-induced folate deficiency may be accompanied by a more severe deficiency of methyl-group donors.…”

Section: Folic Acidmentioning

confidence: 99%

Dietary methyl-consuming compounds and metabolic syndrome

Zhou

et al. 2011

Hypertens Res

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The metabolic syndrome, a major risk factor for type 2 diabetes and cardiovascular disease, is a cluster of metabolic abnormalities including obesity, insulin resistance, hypertension and dyslipidemia. Although systemic oxidative stress and aberrant methylation status are known to have important roles in the development of metabolic syndrome, how they occur remains unclear. The metabolism of methyl-consuming compounds generates reactive oxygen species and consumes labile methyl groups; therefore, a chronic increase in the levels of methyl-consuming compounds in the body can induce not only oxidative stress and subsequent tissue injury, but also methyl-group pool depletion and subsequent aberrant methylation status. In the past few decades, the intake amount of methyl-consuming compounds has substantially increased primarily due to pollution, food additives, niacin fortification and high meat consumption. Thus, increased methyl consumers might have a causal role in the development and prevalence of metabolic syndrome and its related diseases. Moreover, factors that decrease the elimination/ metabolism of methyl-consuming compounds and other xenobiotics (for example, sweat gland inactivity and decreased liver function) or increase the generation of endogenous methyl-consuming compounds (for example, mental stress-induced increase in catecholamine release) may accelerate the progression of metabolic syndrome. Based on current nutrition knowledge and the available evidence from epidemiological, ecological, clinical and laboratory studies on metabolic syndrome and its related diseases, this review outlines the relationship between methyl supply-consumption imbalance and metabolic syndrome, and proposes a novel mechanism for the pathogenesis and prevalence of metabolic syndrome and its related diseases.

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“…A detecção do ácido fólico é realizada por absorção na região do ultra-violeta [15,20], enquanto que para os folatos, a preferência é utilizar a fluorescência [12,14,27,28]. Alguns autores utilizaram esses dois detectores em série para a determinação simultânea de AF e folatos [13,16,26,27].…”

Section: Introductionunclassified

“…Alguns autores utilizaram esses dois detectores em série para a determinação simultânea de AF e folatos [13,16,26,27]. CATHARINO & GODOY [6] observaram que para concentrações baixas de ácido fólico (±20µg/ 100g) é possível a detecção utilizando o detector de fluorescência, porém nenhum sinal foi registrado com concentrações maiores.…”

Section: Introductionunclassified

Avaliação das condições experimentais de CLAE na determinação de ácido fólico em leites enriquecidos

Catharino¹,

Visentainer²,

Godoy³

2003

Ciênc. Tecnol. Aliment.

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O enriquecimento de alimentos com ácido fólico (AF) tem sido incentivado na última década devido, principalmente, a trabalhos que demonstraram uma possível relação da vitamina com as doenças que acometem o tubo neural do feto, isto é, as malformações congênitas, que podem ser ocasionadas pela carência de ácido fólico na alimentação de gestantes [3,7,8,9,11,17,24]. Os produtos escolhidos para o enriquecimento com ácido fólico nos EUA são principalmente os cereais matinais, as farinhas e o macarrão, entre outros [2,25]. No Brasil, o processo de enriquecimento de alimentos com AF vem incluindo uma ampla variedade de produtos, em especial os lácteos.A importância de métodos analíticos apropriados que garantam os níveis de enriquecimento nos produtos é uma preocupação, que deve ser encarada como fator essencial para oferecer a população as quantidades estipuladas nos rótulos dos produtos para benefício comum de todos. Entretanto, a determinação de ácido fólico apresenta muitos desafios, mesmo com a utilização de técni-cas sofisticadas, como é o caso da cromatografia líquida de alta eficiência, os quais incluem o melhoramento nos procedimentos de extração, limpeza e condições cromatográficas mais simples.Há poucos trabalhos sobre o desenvolvimento de metodologias para análise de AF em produtos alimentí-cios [20,21], a maioria das pesquisas foram conduzidas para a determinação de folatos [4,12,18,19,26,28]. Geralmente no processo de extração é feita uma hidrólise alcalina, em virtude do aumento da estabilidade e solubilidade do ácido fólico, para romper os complexos protéicos, seguida de hidrólise enzimática para converter os folatos poliglutâmicos à forma monoglutâmica [4, AVALIAÇÃO DAS CONDIÇÕES EXPERIMENTAIS DE CLAE NA DETERMINAÇÃO DE ÁCIDO FÓLICO EM LEITES ENRIQUECIDOS 1Rodrigo Ramos CATHARINO 2, *, Jesuí Virgilio VISENTAINER 3 , Helena Teixeira GODOY 2 RESUMOA importância que foi atribuída ao ácido fólico recentemente, em virtude de sua ação benéfica ao homem, tem aumentado o interesse dos pesquisadores por esta vitamina. Conseqüentemente, aumentou a preocupação dos analistas em desenvolver metodologias apropriadas para a determinação do ácido fólico (AF) em alimentos enriquecidos ou não com esta vitamina e o controle do mesmo em alimentos enriquecidos. Dentro desse panorama, o objetivo deste trabalho foi de avaliar algumas condições experimentais na determinação e no controle de ácido fólico, por cromatografia líquida de alta eficiência (CLAE), em leites enriquecidos. Utilizando coluna de fase reversa (C 18 ), foram testados 35 diferentes sistemas de eluição isocrática e 11 por gradiente. Os perfis cromatográficos foram monitorados em quatro diferentes comprimentos de onda. Foram realizados estudos sobre a estabilidade das soluções-padrão de AF utilizadas na quantificação. Em relação às etapas pré-cromatográficas, foram avaliadas 15 soluções extratoras. Os procedimentos de extração com soluções alcalinas, pH acima de 7.0, forneceram os melhores resultados. As melhores condições de análise foram obtidas com eluiç...

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Application of an HPLC assay for the determination of folate derivatives in some vegetables, fruits and berries consumed in Finland

Cited by 110 publications

References 18 publications

Impact of strawberries on human health: insight into marginally discussed bioactive compounds for the Mediterranean diet

Impact of strawberries on human health: insight into marginally discussed bioactive compounds for the Mediterranean diet

Dietary methyl-consuming compounds and metabolic syndrome

Avaliação das condições experimentais de CLAE na determinação de ácido fólico em leites enriquecidos

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