Application of an enzyme immunoassay for urinary follicle-stimulating hormone to describe the effects of an acute stressor at different stages of the menstrual cycle in female laboratory macaques

Todd, Heather; Shideler, S. E.; Laughlin, Lisa S.; Overstreet, James W.; Pohl, C. R.; Byrd, William E.; Lasley, Bill L.

doi:10.1002/(sici)1098-2345(1999)48:2<135::aid-ajp5>3.0.co;2-6

Cited by 13 publications

(14 citation statements)

References 27 publications

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“…Ovarian cycles that exhibited prolonged periods of baseline E1C in the absence of an elevation in PdG (Ͼ15 days), that exhibited shortened periods of elevated PdG excretion (Ͻ5 days), or that were of very short duration between episodes of vaginal bleeding (Ͻ22 days) were categorized as abnormal cycles. The normal entrainment of FSH was defined using the FSH␤ subunit profiles of four premenopausal monkeys as assessed by Todd et al [7] using methods identical to those of the present study. The ability of the FSH␤ subunit assay to detect elevations in baseline was validated by monitoring animals following ovariectomy.…”

Section: Discussionmentioning

confidence: 99%

“…All urine samples were analyzed for urinary estrogen and creatinine, and a subset was analyzed for estrogen and progesterone metabolites, and follicle stimulating hormone (FSH␤ subunit) as previously reported [6,7]. Briefly, urinary estrogen and progesterone metabolites were measured by enzyme immunoassay (EIA).…”

Section: Assaysmentioning

confidence: 99%

“…The FSH␤ subunit ELISA was applied according to the method described by Todd et al [7]. Briefly, urine samples were prepared for assay by boiling for 2 min to dissociate dimers.…”

Section: Assaysmentioning

confidence: 99%

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Estrogen and Progesterone Metabolites and Follicle-Stimulating Hormone in the Aged Macaque Female1

Shideler¹,

Gee²,

Chen³

et al. 2001

Biology of Reproduction

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The study presented characterizes the ovarian and pituitary function of the aged female macaque through a complete annual reproductive cycle to compare hormone dynamics during the human and nonhuman primate menopausal transition. Data collected over an entire year from aged macaque females indicated that urinary FSHbeta subunit baseline levels statistically significantly increased in females after age-related abnormal menstrual cycles occurred. These abnormal cycles were followed by anovulation and complete cessation of follicular activity. No statistically significant difference in urinary FSHbeta subunit levels was seen between females that exhibited year-round normal ovarian cycles and those that exhibited seasonal ovarian cycles followed by an interval of anovulation during the nonbreeding season. Basal urinary estrogen metabolite levels were not observed to decrease until ovarian cycles became abnormal and FSHbeta subunit levels began to rise. Early follicular phase circulating inhibin beta levels were statistically significantly reduced only when ovariectomized females were compared to the year-round normally cycling females. A statistically nonsignificant trend toward decreased inhibin secretion, however, was apparent in aged females with normal cycles, aged females with abnormal cycles, anovulatory aged females, and finally, ovariectomized females. Whereas decreased circulating levels of dehydroepiandrosterone sulfate showed a general decline over the 1-yr study period in all groups, they were lowest in the year-round normally cycling group, progressively higher in the normal-to-anovulatory group and abnormal-to-anovulatory group, and highest in the anovulatory group. Finally, the nonbreeding season was associated with the highest number of abnormal cycles, suggesting that onset of complete ovarian senescence in these study macaques was more likely to occur during that time (i.e., females were less likely to return to normal ovarian cycles the following breeding season and more likely to exhibit permanent ovarian quiescence).

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Section: Discussionmentioning

confidence: 99%

Section: Assaysmentioning

confidence: 99%

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Estrogen and Progesterone Metabolites and Follicle-Stimulating Hormone in the Aged Macaque Female1

Shideler¹,

Gee²,

Chen³

et al. 2001

Biology of Reproduction

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“…Initially, we established serum assays for macaque E2 and Po and for their urinary metabolites (22). A urinary assay for the metabolites of FSH (11) also has been validated for assessing macaque pituitary function (23). Previously, there was no reliable assay for monkey chorionic gonadotropin (mCG) and this assay also has been developed (24).…”

Section: Animal Studiesmentioning

confidence: 99%

Biomarkers for assessing human female reproductive health, an interdisciplinary approach.

Lasley

Overstreet

1998

Environ Health Perspect

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Identification of environmental hazards to reproductive health and characterization of the adverse outcomes necessitate a multidisciplinary approach. Epidemiologic studies are required for the identification of adverse health effects in human populations and then to confirm that specific exposures are responsible. Clinical studies are required to develop assays for reproductive biomarkers and to validate these assays prior to their application in the field. Assays for field use must be formatted and streamlined for large-scale applications and, whenever possible, computer algorithms should be developed to interpret biomarker data. Appropriate animal models must be identified, biomarker assays validated for that model, and animal experiments conducted to identify the mode of action and target organ of a putative reproductive toxicant. Finally, in vitro studies at the level of the cell and cell organelle are essential for mechanisms of toxicity to be clearly identified and understood. In this article we describe the interdisciplinary approach that we have developed for study of the effects of environmental agents on female reproductive functions. This effort requires specific skills of toxicologists, epidemiologists, physicians, biochemists, and physiologists.Environ Health Perspect 106 (Suppl 41:955-960 (1998). http.//ehpnetl.niehs.nih.gov/docs/1998/Suppl-4/955-9601asley/abstract.html

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“…An alternative FSH bioassay using cultured rat Sertoli cells noted a cyclic pattern similar to immunoactivity in rhesus monkeys [Matteri et al, 1992]; however, neither of these bioassays was sufficiently sensitive for FSH measurements throughout the reproductive cycle in New World monkeys. Recently, enzyme immunoassays have been developed using monoclonal antibodies against the human FSHβ subunit that are sufficiently sensitive for cyclic FSH measurements in marmoset plasma [Rosenbusch et al, 1997] and macaque urine [Todd et al, 1999]. Alternatively, an RIA developed with recombinant cynomolgus FSH was used in rhesus monkey studies [Ramaswamy et al, 1998;Fraser et al, 1999] and its application was investigated in the squirrel monkey.…”

Section: Introductionmentioning

confidence: 99%

Midcycle and luteal elevations of follicle stimulating hormone in squirrel monkeys (Saimiri boliviensis) during the estrous cycle

et al. 2000

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Follicle stimulating hormone (FSH) has fundamental importance in reproductive function, but its cyclic pattern has not previously been described in the squirrel monkey, due primarily to the lack of a suitable assay. An homologous radioimmunoassay (RIA) based on recombinant cynomolgus FSH measured changes in serum FSH relative to patterns of bioactive luteinizing hormone (LH), estradiol, and progesterone during the estrous cycle. FSH was observed to have a sharp peak during the late follicular phase coincident with the LH surge and then rose again during the luteal phase. Estradiol was low except for the midcycle rise, suggesting an inhibitory relationship. The rat granulosa cell in vitro FSH bioassay confirmed high levels of this hormone. Measurement of FSH in the squirrel monkey has found a pattern different from Old World primates in the luteal phase, which may provide insight into the reproductive mechanisms of this species. Am. J. Primatol. 52:207–211, 2000. © 2000 Wiley‐Liss, Inc.

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Application of an enzyme immunoassay for urinary follicle-stimulating hormone to describe the effects of an acute stressor at different stages of the menstrual cycle in female laboratory macaques

Cited by 13 publications

References 27 publications

Estrogen and Progesterone Metabolites and Follicle-Stimulating Hormone in the Aged Macaque Female1

Estrogen and Progesterone Metabolites and Follicle-Stimulating Hormone in the Aged Macaque Female1

Biomarkers for assessing human female reproductive health, an interdisciplinary approach.

Midcycle and luteal elevations of follicle stimulating hormone in squirrel monkeys (Saimiri boliviensis) during the estrous cycle

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