Application of an ELISA to the Determination of Benalaxyl in Red Wines

Rosso, Ilaria; Giraudi, Gianfranco; Gamberini, Roberta; Baggiani, Claudio; Vanni, Adriano

doi:10.1021/jf9901183

Cited by 14 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous extraction step is usually employed to avoid matrix effects, such as solid-phase microextraction with divinylbenzeneecarboxenepolydimethylsiloxane fibres (Rial-Otero, Yagüe-Ruiz, Cancho-Grande, & Simal-G andara, 2002), liquideliquid extraction (Vaquero-Fern andez et al, 2008), solid-phase extraction with Oasis HLB cartridges (Fontana, Rodríguez, Ramil, Altamirano, & Cela, 2011), dispersive liquideliquid microextraction (Rodríguez-Cabo, Rodríguez, Ramil, & Cela, 2011), or the QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure (Moeder, Bauer, Popp, van Pinxteren, & Reemtsma, 2012;Walorczyk, Drozdzynski, & Gnusowski, 2011). Also, immunoassays have been developed and validated in wines for the analysis of pesticides like benalaxyl (Rosso, Giraudi, Gamberini, Baggiani, & Vanni, 2000), tebufenozide (Irwin, Tolhurst, Jackson, & Gale, 2003), fenhexamid (Mercader & Abad-Fuentes, 2009), or bromopropylate (Ramon-Azc on, S anchez-Baeza, Sanvicens, & Marco, 2009), showing excellent performance.…”

Section: Introductionmentioning

confidence: 99%

Moiety and linker site heterologies for highly sensitive immunoanalysis of cyprodinil in fermented alcoholic drinks

Esteve‐Turrillas¹,

Mercader²,

Agulló³

et al. 2015

Food Control

View full text Add to dashboard Cite

1Cyprodinil is a new-generation anilinopyrimidine fungicide widely used in crop protection 2 and frequently found in fruits. In this study, novel derivatives of cyprodinil with linker site 3 heterologies were synthesized and employed in order to produce antibodies with enhanced affinity. 4Moreover, moiety-heterologous haptens were designed and prepared for assay sensitivity 5 improvement. Two competitive enzyme-linked immunosorbent assays for the analysis of this active 6 substance were developed using direct and indirect formats, achieving IC 50 values around 0.15 7 µg/L. Analytical figures of merit and usability of the optimized assays were evaluated with wine and 8 cider as model food processed matrices. The obtained recoveries were from 90% to 120%, and the 9 limit of quantification was in the 1 to 5 µg/L range. Finally, a monitoring study (n=150) was 10

show abstract

Section: Introductionmentioning

confidence: 99%

Moiety and linker site heterologies for highly sensitive immunoanalysis of cyprodinil in fermented alcoholic drinks

Esteve‐Turrillas¹,

Mercader²,

Agulló³

et al. 2015

Food Control

View full text Add to dashboard Cite

show abstract

“…The activity of BX is mainly attributed to the (−)‐R‐enantiomer 11. A number of methods have been reported for the determination of BX residues in various samples, such as enzyme‐linked immunosorbent assay in water and wine12, 13 and gas chromatography in vegetables and fruits 14. The stereoselective degradation of BX in rabbit plasma,11 soils, and cucumber plants15 were investigated by chiral high‐performance liquid chromatography.…”

Section: Introductionmentioning

confidence: 99%

Stereoselective metabolism of benalaxyl in liver microsomes from rat and rabbit

et al. 2011

View full text Add to dashboard Cite

Benalaxyl (BX), methyl-N-phenylacetyl-N-2,6-xylyl alaninate, is a potent acylanilide fungicide and consist of a pair of enantiomers. The stereoselective metabolism of BX was investigated in rat and rabbit microsomes in vitro. The degradation kinetics and the enantiomer fraction (EF) were determined using normal high-performance liquid chromatography with diode array detection and a cellulose-tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase (CDMPC-CSP). The t(1/2) of (-)-R-BX and (+)-S-BX in rat liver microsomes were 22.35 and 10.66 min of rac-BX and 5.42 and 4.03 of BX enantiomers. However, the t(1/2) of (-)-R-BX and (+)-S-BX in rabbit liver microsomes were 11.75 and 15.26 min of rac-BX and 5.66 and 9.63 of BX enantiomers. The consequence was consistent with the stereoselective toxicokinetics of BX in vitro. There was no chiral inversion from the (-)-R-BX to (+)-S-BX or inversion from (+)-S-BX to (-)-R-BX in both rabbit and rat microsomes. These results suggested metabolism of BX enantiomers was stereoselective in rat and rabbit liver microsomes.

show abstract

“…It can be absorbed by the roots, stems, and leaves and apoplastic movement occurs acropetally to all parts of the plant. In agriculture, it is extensively used to control late blights of potatoes and tomatoes and downy mildews of hops, vines, lettuce, onions, soya beans, tobacco, and other crops, caused by fungi of the family Peronosporaceae − . Benalaxyl molecules consist of a pair of enantiomers (Figure ), R -enantiomer with left optical (−) rotation and S -enantiomer with right optical (+) rotation, because of the presence of the asymmetric carbon in the alkyl moiety .…”

Section: Introductionmentioning

confidence: 99%

Enantioselective Acute Toxicity and Bioaccumulation of Benalaxyl in Earthworm (Eisenia fedtia)

Liu²,

Diao³

et al. 2009

J. Agric. Food Chem.

View full text Add to dashboard Cite

The enantioselectivities of individual enantiomers of benalaxyl in acute toxicity and bioaccumulation in earthworm ( Eisenia fedtia ) were studied. The acute toxicity was tested by paper contact test. After 48 h of exposure, the calculated LC(50) values of the R-(-)-form, rac-form, and S-(+)-form were 4.99, 5.08, and 6.66 microg/cm(2), respectively. After 72 h of exposure, the calculated LC(50) values were 1.23, 1.73, and 2.45 microg/cm(2), respectively. Therefore, the acute toxicity of benalaxyl enantiomers was enantioselective. A method for determining residues of the two enantiomers of benalaxyl in earthworm tissue by high-performance liquid chromatography based on cellulose tri-(3,5-dimethylphenyl-carbamate) chiral stationary phase was developed. During the bioaccumulation experiment, the enantiomer fraction in earthworm tissue was maintained approximately at 0.6, whereas enantiomer fraction in spiked soil was maintained at 0.5; in other words, the bioaccumulation of benalaxyl was enantioselective in earthworm tissue. Peak-shaped accumulation curves were observed for both enantiomers, and the calculated biota to soil accumulations (kg dry kg(-1) wet weight) at steady state were below 1 for both enantiomers. During the elimination experiment, 79.0% of R-(-)-enantiomer and 89.6% of S-(+)-enantiomer in earthworm tissue were eliminated within 2 days.

show abstract

Application of an ELISA to the Determination of Benalaxyl in Red Wines

Cited by 14 publications

References 9 publications

Moiety and linker site heterologies for highly sensitive immunoanalysis of cyprodinil in fermented alcoholic drinks

Moiety and linker site heterologies for highly sensitive immunoanalysis of cyprodinil in fermented alcoholic drinks

Stereoselective metabolism of benalaxyl in liver microsomes from rat and rabbit

Enantioselective Acute Toxicity and Bioaccumulation of Benalaxyl in Earthworm (Eisenia fedtia)

Contact Info

Product

Resources

About