Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

Anton, Milan; Moráňová, Ludmila; Hrstka, Roman; Bartošík, Martin

doi:10.1039/c9ay02383f

Cited by 14 publications

(10 citation statements)

References 34 publications

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“…In the next attempt, 61 cervical samples for both genotypes were screened and validated with real-time PCR and commercial HPV tests such as INNO-LiPA and COBAS. The system had 90% accuracy [ 80 ]. Recently, Ramírez-Chavarría et al [ 81 ] proposed a LAMP-based low-cost electrochemical sensor for specific detection of a SARS-CoV-2 gene sequence, in which RT-LAMP is performed on a disposable test strip consisting of a screen-printed electrode.…”

Section: Lamp Detection Methodsmentioning

confidence: 99%

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

2022

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Seeking optimized infectious pathogen detection tools is of primary importance to lessen the spread of infections, allowing prompt medical attention for the infected. Among nucleic-acid-based sensing techniques, loop-mediated isothermal amplification is a promising method, as it provides rapid, sensitive, and specific detection of microbial and viral pathogens and has enormous potential to transform current point-of-care molecular diagnostics. In this review, the advances in LAMP-based point-of-care diagnostics assays developed during the past few years for rapid and sensitive detection of infectious pathogens are outlined. The numerous detection methods of LAMP-based biosensors are discussed in an end-point and real-time manner with ideal examples. We also summarize the trends in LAMP-on-a-chip modalities, such as classical microfluidic, paper-based, and digital LAMP, with their merits and limitations. Finally, we provide our opinion on the future improvement of on-chip LAMP methods. This review serves as an overview of recent breakthroughs in the LAMP approach and their potential for use in the diagnosis of existing and emerging diseases.

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Section: Lamp Detection Methodsmentioning

confidence: 99%

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

2022

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“…Our team has published several research articles reporting fast and simple LAMP‐based assays combined with electrochemical detection (EC‐LAMP), targeting HPV16/18 genotypes in both cervical cell lines and cervical smears from women with precancerous lesions (Figure 1). 79–82 After development and optimization of the assay, 80 the EC‐LAMP was applied into a cohort of 61 clinical samples to evaluate its performance and compared it with PCR‐based methods and with INNO‐LiPA genotyping assay 81 . Good specificity and negative and positive predictive values were reached, all over 90%.…”

Section: Isothermal Amplification Techniques As Rapid Alternatives To...mentioning

confidence: 99%

Advanced technologies towards improved HPV diagnostics

Bartosik,

Moranova,

Izadi

et al. 2024

Journal of Medical Virology

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Persistent infection with high‐risk types of human papillomaviruses (HPV) is a major cause of cervical cancer, and an important factor in other malignancies, for example, head and neck cancer. Despite recent progress in screening and vaccination, the incidence and mortality are still relatively high, especially in low‐income countries. The mortality and financial burden associated with the treatment could be decreased if a simple, rapid, and inexpensive technology for HPV testing becomes available, targeting individuals for further monitoring with increased risk of developing cancer. Commercial HPV tests available in the market are often relatively expensive, time‐consuming, and require sophisticated instrumentation, which limits their more widespread utilization. To address these challenges, novel technologies are being implemented also for HPV diagnostics that include for example, isothermal amplification techniques, lateral flow assays, CRISPR‐Cas‐based systems, as well as microfluidics, paperfluidics and lab‐on‐a‐chip devices, ideal for point‐of‐care testing in decentralized settings. In this review, we first evaluate current commercial HPV tests, followed by a description of advanced technologies, explanation of their principles, critical evaluation of their strengths and weaknesses, and suggestions for their possible implementation into medical diagnostics.

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“…It requires 4-6 primers which makes the reaction highly sequence-specific and generates products of variable lengths, ranging from hundreds to few thousand bp. LAMP is widely used for pathogen analysis not only in a fluorescent or colorimetric format [117][118][119][120], but also in electrochemical assays to detect viruses, such as human papillomavirus (HPV) [121][122][123][124] and hepatitis virus B [125], and bacteria (E. coli, Salmonella, etc.) [126,127].…”

Section: Iat-based Ec Biosensorsmentioning

confidence: 99%

“…Indeed, the access to human clinical samples does not represent an insurmountable obstacle, as demonstrated by EC works that successfully employed their assays into patient samples with satisfactory results [69,70,73,75,78,141]. It would be highly beneficial if some EC strategy is applied to a larger cohort of patients (tens or even hundreds) and compared with a standard method of detection with rigorous statistics; this feature of EC assays is currently in its infancy [99,124,142], but would increase an impact of electrochemistry in clinical diagnostics.…”

Section: Outlook and Conclusionmentioning

confidence: 99%

Electrochemical biosensors for analysis of DNA point mutations in cancer research

et al. 2022

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Cancer is a genetic disease induced by mutations in DNA, in particular point mutations in important driver genes that lead to protein malfunctioning and ultimately to tumorigenesis. Screening for the most common DNA point mutations, especially in such genes as TP53, BRCA1 and BRCA2, EGFR, KRAS, or BRAF, is crucial to determine predisposition risk for cancer or to predict response to therapy. In this review, we briefly depict how these genes are involved in cancer, followed by a description of the most common techniques routinely applied for their analysis, including high-throughput next-generation sequencing technology and less expensive low-throughput options, such as real-time PCR, restriction fragment length polymorphism, or high resolution melting analysis. We then introduce benefits of electrochemical biosensors as interesting alternatives to the standard methods in terms of cost, speed, and simplicity. We describe most common strategies involved in electrochemical biosensing of point mutations, relying mostly on PCR or isothermal amplification techniques, and critically discuss major challenges and obstacles that, until now, prevented their more widespread application in clinical settings.

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Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

Abstract: Clinical evaluation of rapid, simple and inexpensive electrochemical assay using LAMP amplification for detection of two most oncogenic human papillomavirus types, HPV16 and HPV18.

Cited by 14 publications

References 34 publications

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

Advanced technologies towards improved HPV diagnostics

Electrochemical biosensors for analysis of DNA point mutations in cancer research

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