Application of an automated tandem high-performance liquid chromatographic system to peptide mapping of genetic variants of human serum albumin

Takahashi, Nobuhiro; Takahashi, Yōko; Ishioka, Noriaki; Blumberg, Baruch S.; Putnam, Frank W.

doi:10.1016/0021-9673(86)80072-3

Cited by 40 publications

(45 citation statements)

References 12 publications

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“…Previous study ofthe CNBr digest by isoelectric focusing had indicated the amino acid substitution was in CB5, a large CNBr fragment (residues 330-446). However, despite extensive anjlysis the variant peptide was not identified in the tryptic peptide profile produced by the automated tandem HPLC system (9,12). In the present work the total albumin (albumin A and albumin Hir-1, denoted A/Hir-1) was purified by the two-step procedure (13) (Fig.…”

Section: Resultsmentioning

confidence: 93%

“…This exchange accords with the +2 slow mobility of Hir-2 and corresponds to a single base change in the codon GAA to AAA. (9). T48A* and T48B* denote the two new (variant) peptides containing the amino acid substitution in albumin Hir-l; T50A* and T5OB* are the variant peptides for albumin Tochigi, and T5OAt and T50Bt are the variant peptides for albumin Hir-2.…”

Section: Resultsmentioning

confidence: 99%

“…For want of structural information such variants generally have been assigned ethnic or geographical names. Recently we (4,(9)(10)(11)(12)(13)(14)(15) and others (16)(17)(18)(19)(20)(21) have determined the structural change in a number of variant albumins. We have now completed the structural study of the five rare types of inherited albumin variants discovered in the Biochemical Genetics Study of the Radiation Effects Research Foundation (RERF); this surveyed some 30 blood and plasma polypeptides in a cohort of 15,581 unrelated children in Nagasaki and Hiroshima (2).…”

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confidence: 99%

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Point substitutions in Japanese alloalbumins.

Arai

Madison²,

Huss³

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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We have completed the structural study of five rare types of inherited albumin variants (alloalbumins) discovered in the Biochemical Genetics Study of 15,581 unrelated children in Hiroshima and Nagasaki. We have also identified the structural chane inl five other alloalbumin specimens detected during clinical electrophoresis of sera from Japanese living near Tokyo. Each of the five albumin variants from Nagasaki and Hiroshima has a single amino acid substitution. AlU of these substitutions differ, and none has been reported in non-Japanese populations. No instances of proalbumin variants or of albumin B (the most frequent alloalbumins in Caucasians) were detected in the children in Hiroshima and Nagasaki. However, one instance of a variant proalbumin and two examples of albumin B occurred in Japanese from the vicinity of Tokyo. In addition a previously unreported point substitution was found in albumin Tochigi, which is present in two unrelated persons from Tochigi prefecture. Four of the point mutations in the Japanese alloalbumins are in close proximity in a short segment of the polypeptide chain (residues 354-382) in which three additional point substitutions have been reported in diverse populations. These results, combined with earlier data, suggest that point substitutions are grouped in certain segments of the albumin molecule.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Point substitutions in Japanese alloalbumins.

Arai

Madison²,

Huss³

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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“…All individuals were heterozygous for the trait except for one homozygous Naskapi donor (12,13). Purification of the albumin from serum samples was described by Takahashi et al (12).…”

Section: Methodsmentioning

confidence: 99%

“…Previously described methods used for the characterization of the albumin variants and for structural study include the following: (i) NaDodSO4/PAGE of the carboxymethylated albumins and CNBr digests (13), (it) automated tandem HPLC mapping of tryptic peptides (12), (iii) HPLC mapping and isoelectric focusing of CNBr peptides (13), and (iv) amino acid-sequence analysis of the intact purified albumin and of tryptic and CNBr peptides with the Beckman sequencer model 890C (12), radioautography with 63Ni2+ (8). RESULTS tTo whom reprint requests should be addressed.…”

Section: Methodsmentioning

confidence: 99%

Structural changes and metal binding by proalbumins and other amino-terminal genetic variants of human serum albumin.

Takahashi

Putnam

1987

Proc. Natl. Acad. Sci. U.S.A.

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Proalbumins are rare genetic variants of human serum albumin containing a basic propeptide that is not removed during post-transcriptional processing because of a mutation in the site of excision, an Arg-Arg sequence. We have identified the amino acid substitutions in three different types of proalbumins designated Gainesville, Taipei, and Takefu. The first two proalbumins are identical to previously described proalbumins of the Christchurch and Lille types, respectively, and exhibit the characteristic properties ofsusceptibility to tryptic cleavage and of lower metal-binding affinity. Takefu is a third

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