Application of an association of yeast and lactic acid bacteria to bioencapsulate carotenoids in Daphnia magna (Straus, 1820)

Abstract

Freshwater zooplankton, which is commonly used as a starting fish feed, has a low content of essential compounds such as carotenoids. The possibility of increasing the productivity of carotenogenic yeast, Rhodotorula glutinis, for further bioencapsulation of zooplankton Daphnia magna is shown. An association of a UV irradiated yeast strain with lactic acid bacteria was cultivated for this purpose. This permitted intensifying the carotenogenesis of yeast, and the content of â-carotene increased by 1.7 times, and that of torularodine by 2.3 times compared with the native monoculture. The use of the association of microorganisms as a feed substrate in the cultivation of Daphnia magna provided both an increase in the carotenoid content in the investigated crustaceans more than 8 times, and their biomass by 2.5 times compared with the standard feed of Saccharomyces cerevisiae.

Molecular farming: Expanding the field of edible vaccines for sustainable fish aquaculture

“…Crustaceans, namely Artemia , Moina , and Daphnia , commonly used as fish food in the early stages of fish life, represent a third alternative to the abovementioned strategies. Owing to their capacity to naturally consume bacteria, yeasts, or microalgae, these crustaceans are commonly used as carriers for nutritional or antibacterial agents 53–58 . Additionally, due to their encapsulating capacities, they have been previously used for oral delivery of various probiotic strains, resulting in improved health, overall growth, and fitness of different fish species 59–61 .…”