Application of amniotic fluid stem cells in repairing sciatic nerve injury in minipigs

Su, Cheng‐Kuan; Chang, Li-Hsun; Kao, Chien-Yu; Lee, Dong Hoon; Cho, Kuan-Hung; Li, Kuo; Chang, Hsiu‐Hua; Wang, Yao‐Horng; Chiu, Ing‐Ming

doi:10.1016/j.brainres.2017.11.010

Cited by 18 publications

(12 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They present good results when used in the treatment of chronic kidney disease, 43 infertility, 44 ophthalmic lesions, 45 , 46 burns and skin wounds, 47 , 48 osteoarthritis, 49 fasciitis and tendinitis, 50 ligament injuries 51 and nervous pathologies. 52 However, this cell type is still little explored in the treatment of spinal cord injuries in dogs, 37 , 39 and our work is the first to use them in dogs with chronic, non-iatrogenic intervertebral disc disease.…”

Section: Discussionmentioning

confidence: 94%

Treatment of Chronic Spinal Cord Injury in Dogs Using Amniotic Membrane-Derived Stem Cells: Preliminary Results

Orlandin

Gomes

Leandro

et al. 2021

SCCAA

View full text Add to dashboard Cite

Introduction: Intervertebral disc diseases (IVDD) represent the majority of neurological attendance and responsible for the most cases of paralysis in dogs. Treatments currently used do not show satisfactory results in patients with more severe and chronic neurological manifestations. Methods: To promote nerve and muscular recovery, as well as improve quality of life, we aimed to create a double-blind test method, associating spinal decompression surgery and allogeneic transplantation of amniotic membrane-derived stem cells (AMSCs) in dogs with chronic IVDD. Cells were characterized as fetal mesenchymal cells and safe for application. Eight animals completed the experiment: stem cell applications were made in four animals that had previously undergone an unsuccessful surgical procedure ("SC group", n = 4); two animals were submitted to surgery, followed by applications of stem cells ("Surgery + SC", n = 2); two other animals were submitted to surgery, followed by the application of saline solution ("Surgery + placebo", n = 2). During the surgical procedure, a topical application was performed on the lesion and after fifteen and forty-five days another two applications were made via epidural. Animals were monitored biweekly and reassessed three months after surgery, by functional tests and magnetic resonance exams. Results: Some animals presented significant neurological improvement, such as the recovery of nociception and ability to remain on station. Despite the need further studies, until the present moment, cell therapy has been feasible and has no harmful effects on animals. Conclusion:The protocol of preclinical trial showed the association with decompressive surgery and cell transplantation in dogs with thoracolumbar IVDD proved feasible, and it was possible to observe neurological improvement after treatment. No tissue improvement through MRI was found. The double-blind test guaranteed reliability of the evaluations and results obtained that, even with a small sample size, generated satisfactory results for the animals and owners.

show abstract

Section: Discussionmentioning

confidence: 94%

Treatment of Chronic Spinal Cord Injury in Dogs Using Amniotic Membrane-Derived Stem Cells: Preliminary Results

Orlandin

Gomes

Leandro

et al. 2021

SCCAA

View full text Add to dashboard Cite

show abstract

“…Indeed, previous studies have evaluated regeneration in pigs using ultrasonography, magnetic resonance and diffusion tensor imaging-based tractography, sensory and motor evoked potentials, nerve conduction velocity, muscle activation, and nerve tract tracing. [75][76][77][78][79][80][81] Electrophysiological functional assessments are frequently used to indirectly evaluate nerve regeneration and the extent of target muscle functionality at specific time points. [82][83][84][85] The evaluation and interpretation of functional recovery via nerve electrical stimulation and nerve/muscle recording can be technically challenging due to hardware issues, over-or under-sampling data, stimulus artifact, anatomical variation, or poor functional recovery.…”

Section: Discussionmentioning

confidence: 99%

A Porcine Model of Peripheral Nerve Injury Enabling Ultra-Long Regenerative Distances: Surgical Approach, Recovery Kinetics, and Clinical Relevance

Burrell¹,

Browne²,

Dutton³

et al. 2020

Neurosurg.

View full text Add to dashboard Cite

BACKGROUND Millions of Americans experience residual deficits from traumatic peripheral nerve injury (PNI). Despite advancements in surgical technique, repair typically results in poor functional outcomes due to prolonged periods of denervation resulting from long regenerative distances coupled with slow rates of axonal regeneration. Novel surgical solutions require valid preclinical models that adequately replicate the key challenges of clinical PNI. OBJECTIVE To develop a preclinical model of PNI in swine that addresses 2 challenging, clinically relevant PNI scenarios: long segmental defects (≥5 cm) and ultra-long regenerative distances (20-27 cm). Thus, we aim to demonstrate that a porcine model of major PNI is suitable as a potential framework to evaluate novel regenerative strategies prior to clinical deployment. METHODS A 5-cm-long common peroneal nerve or deep peroneal nerve injury was repaired using a saphenous nerve or sural nerve autograft, respectively. Histological and electrophysiological assessments were performed at 9 to 12 mo post repair to evaluate nerve regeneration and functional recovery. Relevant anatomy, surgical approach, and functional/histological outcomes were characterized for both repair techniques. RESULTS Axons regenerated across the repair zone and were identified in the distal stump. Electrophysiological recordings confirmed these findings and suggested regenerating axons reinnervated target muscles. CONCLUSION The models presented herein provide opportunities to investigate peripheral nerve regeneration using different nerves tailored for specific mechanisms of interest, such as nerve modality (motor, sensory, and mixed fiber composition), injury length (short/long gap), and total regenerative distance (proximal/distal injury).

show abstract

“…In addition, human amniotic membrane epithelial cells (hAEC) ( Jin et al, 2015 ), hAMSC ( Chen et al, 2022 ), hAF stem cells ( Su et al, 2018 ), or hUC-MSC ( Bojanic et al, 2020 ) have been tested alone or coupled to additional scaffolds like PCL or chitosan or embedded in fibrin hydrogel-containing polylactate (PLA) nerve conduits ( Su et al, 2018 ). For sciatic nerve injury models, Schwann cell proliferation and migration were used as in vitro assays ( Chen et al, 2022 ).…”

Section: Specific Sections Dedicated To Pnd Applicationsmentioning

confidence: 99%

General consensus on multimodal functions and validation analysis of perinatal derivatives for regenerative medicine applications

Pozzobon

D’Agostino

Roubelakis

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Perinatal tissues, such as placenta and umbilical cord contain a variety of somatic stem cell types, spanning from the largely used hematopoietic stem and progenitor cells to the most recently described broadly multipotent epithelial and stromal cells. As perinatal derivatives (PnD), several of these cell types and related products provide an interesting regenerative potential for a variety of diseases. Within COST SPRINT Action, we continue our review series, revising and summarizing the modalities of action and proposed medical approaches using PnD products: cells, secretome, extracellular vesicles, and decellularized tissues. Focusing on the brain, bone, skeletal muscle, heart, intestinal, liver, and lung pathologies, we discuss the importance of potency testing in validating PnD therapeutics, and critically evaluate the concept of PnD application in the field of tissue regeneration. Hereby we aim to shed light on the actual therapeutic properties of PnD, with an open eye for future clinical application. This review is part of a quadrinomial series on functional/potency assays for validation of PnD, spanning biological functions, such as immunomodulation, anti-microbial/anti-cancer, anti-inflammation, wound healing, angiogenesis, and regeneration.

show abstract

Application of amniotic fluid stem cells in repairing sciatic nerve injury in minipigs

Cited by 18 publications

References 45 publications

Treatment of Chronic Spinal Cord Injury in Dogs Using Amniotic Membrane-Derived Stem Cells: Preliminary Results

Treatment of Chronic Spinal Cord Injury in Dogs Using Amniotic Membrane-Derived Stem Cells: Preliminary Results

A Porcine Model of Peripheral Nerve Injury Enabling Ultra-Long Regenerative Distances: Surgical Approach, Recovery Kinetics, and Clinical Relevance

General consensus on multimodal functions and validation analysis of perinatal derivatives for regenerative medicine applications

Contact Info

Product

Resources

About