Application of AFLP markers for QTL mapping in the rabbit

Abstract

Two rabbit (Oryctolagus cuniculus) inbred strains (AX/JU and IIIVO/JU) have been used for genetic analysis of quantitative traits related to dietary cholesterol susceptibility. Application of the AFLP (amplified fragment length polymorphism) technique with 15 primer combinations revealed 226 polymorphisms between the 2 inbred strains. A total of 57 animals from a backcross progeny (IIIVO/JU x [IIIVO/JU x AX/JU]F1) were available for the genetic analysis. These backcross animals were fed a commercial pelleted diet fortified with 0.3% w/w cholesterol during a test period that lasted five weeks. A male genetic map could be constructed, consisting of 12 linkage groups and 103 AFLP markers. Linkage analysis between the cholesterol-related traits and marker loci revealed a significant LOD score for the relative weight of adrenal glands in males (LOD score = 3.83), whereas suggestive linkages were found for basal serum total cholesterol levels in females (LOD score = 2.69), for serum total cholesterol response (area under the curve) in males (LOD score = 2.21), and for hematocrit in males (LOD score = 3.24).

Application of rabbits in biomedical research: a review.

“…AX/JU and IIIVO/JU have been used for genetic analysis of quantitative traits related to dietary cholesterol susceptibility. Application of the AFLP technique with 15 primer combinations revealed 226 polymorphisms between the two inbred strains (Van Haeringen et al, 2002). Recent data point to the fact that dietary cholesterol induces neuronal accumulation of Alzheimer's like beta-amyloid in New Zealand White rabbits, and copper supplementation in drinking water resulted in fibrillar type beta-amyloid accumulation (Sparks, 2004).…”

Genomics Meets Biodiversity: Advances in Molecular Marker Development and Their Applications in Plant Genetic Diversity Assessment

“…Secondly, the produced banding patterns for each technique are treated during the evaluation process as dominant markers, and the methods are described accordingly. Thus, it is difficult to distinguish heterozygous individuals from homozygous ones with respect to the band-presence allele, unless exact genotypes can be inferred from pedigree studies (van Haeringen et al, 2002). However, this assumption may be challenged in future and methods appropriately modified if the technical nature of each marker system is properly recognized.…”

Genome Mapping and Genomics in Domestic Animals

“…A first attempt to develop large-scale molecular genetic markers was based on amplified fragment-length polymorphism (AFLP) techniques (van Haeringen et al 2001(van Haeringen et al , 2002. The major advantage of this method is the possibility to develop hundreds of markers without any prior knowledge of the genome (Vos et al 1995).…”