Application of adaptive laboratory evolution for Yarrowia lipolytica: A comprehensive review

Recent Advances and Multiple Strategies of Monoterpenoid Overproduction in Saccharomyces cerevisiae and Yarrowia lipolytica

“…Adaptive evolution (ALE) enables microorganisms to naturally change, via long-term cultivation under controlled laboratory conditions with specific selection pressures such as high temperatures, low pH, high salinity, or the presence of particular chemicals. 66 This approach leverages the principles of natural selection, enabling strains to autonomously undergo genetic modifications in response to adaptive stress, thus securing survival and growth advantages in specified environments. 67 Here, ALE involves subjecting yeast to specific survival pressures to drive its evolution toward improved growth and production, a technique that has been successfully applied in both E. coli and yeasts.…”

Yarrowia lipolytica Yeast: A Treasure Trove of Enzymes for Biocatalytic Applications—A Review

“…Yarrowia lipolytica is a versatile yeast species known for its broad and diverse application over the years, with its robust enzymatic capabilities and significant biocatalytic potential [4]. Moreover, the processes based on this yeast have GRAS (Generally Recognized As Safe) status, and the yeast biomass has been defined as a safe novel food by the EFSA (European Food Safety Authority), allowing it to be used as food for people over the age of 3, as well as a dietary supplement [5,6].…”