Application of acute phase protein measurements 
in veterinary clinical chemistry

Petersen, Henrik H.; Nielsen, Jens Peter; Heegaard, Peter M. H.

doi:10.1051/vetres:2004002

Cited by 801 publications

(875 citation statements)

References 162 publications

(263 reference statements)

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“…Similarly, two peaks of high protein levels were obtained for Hp determinations, appearing 1 and 2 weeks after CRP increments, respectively, and showing the later/slow behavior of Hp after being in contact with PCV2. A slow behavior of Hp in comparison with CRP after an inflammatory stimulus has been reported before in serum samples (Petersen et al, 2004), with Hp being characterized as a second-line APP. However, neither kinetic comparison of different APP in saliva samples nor weekly longitudinal experiments, which allow comparison with our results, have been reported until now, to the authors' knowledge.…”

Section: Discussionsupporting

confidence: 68%

Longitudinal analysis of acute-phase proteins in saliva in pig farms with different health status

Gutiérrez

Cerón

Fuentes

et al. 2012

Animal

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This study assesses the utility of saliva samples to monitor the time course of the acute-phase response to different viruses in pigs under field conditions by using time-resolved immunofluorometric assays (TR-IFMA). A total of 30 pigs from three different farms, located in Southeast Spain, were used. Farm 1 had outbreaks of porcine circovirus type 2, farm 2 had infections with porcine reproductive and respiratory syndrome virus and farm 3 had concomitant infections with both viruses. Serology was used to determine the time of seroconversion of pigs to two different pathogens. The levels of two acute-phase proteins (APPs), C-reactive protein (CRP) and haptoglobin (Hp), were measured in saliva and serum samples and compared with pig's serology. Kinetic curves of both APPs across the study obtained in saliva samples were similar to those of serum, with R of 0.68 and 0.78 for CRP and Hp, respectively. The median CRP and Hp concentrations in saliva were higher around the theorized time of infection, according to previous experimental studies, and at seroconversion of animals. CRP increments were apparent 1 week before the increments obtained in Hp. These findings indicate that salivary APP concentrations, by using TR-IFMA, can be used in longitudinal studies as non-invasive early indicators of health status.

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Section: Discussionsupporting

confidence: 68%

Longitudinal analysis of acute-phase proteins in saliva in pig farms with different health status

Gutiérrez

Cerón

Fuentes

et al. 2012

Animal

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“…Research interest has been focussed on the study of pig APP response in experimental acute inflammation and bacterial infections, as well as in farm animal manipulations [7,18,21,28,31,39,42]. Although there have been studies about virus infections in pigs [2,11,35,38], little is known about the APP response elicited by these pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…APP were first characterised in humans [20], rodents [19,22] and later in other animal species, such as pigs [13,21]. Clinical use of APP determination has been proposed in veterinary medicine to determine the health status of farm animals in various production processes [14,28,39]. Increases in haptoglobin (Hp), C-reactive protein (CRP) and serum amyloid A in pigs with induced inflammation or bacterial infection have been reported [13,18,21].…”

Section: Introductionmentioning

confidence: 99%

Pig Major Acute-Phase Protein and apolipoprotein A-I responses correlate with the clinical course of experimentally induced African Swine Fever and Aujeszky's disease

et al. 2007

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-In the present work, we studied the acute phase protein response after experimental virus infection in pigs. The animals were experimentally infected with African Swine Fever (ASF) or Aujeszky's disease (AD) viruses. The clinical course of ASF infection correlated with increasingly high levels of pig Major Acute-phase Protein (pig-MAP) (mean value of 6 mg/mL on day 6 post infection (p.i.), from 6 to 9 times higher than day 0) and sharp apolipoprotein A-I (apo A-I) decrease (mean value of 0.5 mg/mL, from 4 to 10 times lower than day 0 on day 4 p.i.). AD-clinical signs appeared at day 3 p.i., both in vaccinated (moderate clinical signs) and non-vaccinated pigs (severe outcome within 48 h p.i.). Pig-MAP and apo A-I profiles also followed clinical signs (changing from 0.70 mg/mL to around 3 mg/mL and from around 3 mg/mL to 0.96 mg/mL, respectively in non-vaccinated animals), with minor changes in concentration in the vaccinated group. Haptoglobin levels significantly increased in ASF and AD infected animals (mean maximum values of 2.77 and 3.96 mg/mL, respectively). Minor differences for the C-Reactive Protein in the case of ASF were observed, whereas its concentration increased more than 7 times in AD-infection. The albumin level was not modified in either case. The correlation of clinical signs to our data suggests the potential use of pig-MAP and apo A-I in monitoring infections in swine.

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“…The identification of appropriate biomarkers would improve the situation as biomarkers can be used to diagnose infection [15,28,38,50] and, potentially, are also applicable as markers for breeding selection. In case of respiratory tract infections local disease markers such as proteins present in bronchoalveolar lavage fluid (BALF) are likely to be most meaningful [13,35,53].…”

Section: Introductionmentioning

confidence: 99%

Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance toActinobacillus pleuropneumoniaeinfection

D¹,

Buettner²,

Naim³

et al. 2009

Vet. Res.

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-Biomarkers facilitating both pathogen-independent diagnosis of respiratory health and breeding selection of pigs with increased resistance to respiratory tract infections would be of considerable interest to the pig industry. Following this concept we performed a comparative glycoproteome analysis of bronchoalveolar lavage fluid (BALF) from healthy pigs and pigs 4 days (acute) and 20 days (chronic) after an experimental infection with Actinobacillus pleuropneumoniae. In order to identify possible differences in BALF glycoprotein patterns we investigated pigs of three different breeding lines (German Landrace, Piétrain, Hampshire). In total, 12 glycosylated proteins (alpha-1-acid glycoprotein, fetuin A, properdin, haptoglobin precursor, haptoglobin, hemoglobin, hyaluronidase, inter-alpha-trypsin inhibitor family heavy chain-related protein, alpha-1-antichymotrypsin 3, pulmonary surfactant-associated protein D (SP-D), transferrin, and alpha-1B-glycoprotein) were identified as being differentially expressed depending on the health status of the animal. Fetuin A levels were consistently low in chronically infected pigs thereby being a potential marker for chronic infection. Hyaluronidase levels were consistently high in all pigs after experimental infection independent on isolation of the pathogen thereby being a potential marker for previous pathogen contact and latent infection. High levels of fetuin A as well as low levels of haptoglobin and pulmonary SP-D correlated with the absence of lung lesions in pigs of the Hampshire breeding line, implying a potential application as selection markers for breeding programmes.porcine respiratory disease / glycoprotein analysis / bronchoalveolar lavage fluid / biomarker

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Application of acute phase protein measurements in veterinary clinical chemistry

Cited by 801 publications

References 162 publications

Longitudinal analysis of acute-phase proteins in saliva in pig farms with different health status

Longitudinal analysis of acute-phase proteins in saliva in pig farms with different health status

Pig Major Acute-Phase Protein and apolipoprotein A-I responses correlate with the clinical course of experimentally induced African Swine Fever and Aujeszky's disease

Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance toActinobacillus pleuropneumoniaeinfection

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