Application of activated charcoal and nanocarbon to callus induction and plant regeneration in aromatic rice (<i>Oryza sativa</i> L.)

Chutipaijit, Sutee; Sutjaritvorakul, Thanawat

doi:10.1080/09542299.2017.1418184

Cited by 20 publications

(13 citation statements)

References 56 publications

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“…Rice is an important cereal crop, which fulfills the dietary needs of billions of people globally (Mahajan, Sekhon, Singh, Kaur, & Sidhu, ), whereas fragrant rice is well praised and liked by the consumers due to its high grain quality, strong aroma, and good taste (Chutipaijit & Sutjaritvorakul, ; Sasmal & Pal, ). “Basmati” and “Jasmine” are the typical fragrant rice types, which are world‐famous due to their long‐grain and fragrant characters.…”

Section: Introductionmentioning

confidence: 99%

Application of γ‐aminobutyric acid (GABA) and nitrogen regulates aroma biochemistry in fragrant rice

Xie

Ashraf

Zhong

et al. 2019

Food Science & Nutrition

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The 2‐acetyl‐1‐pyrroline (2AP) is a key aroma compound in fragrant rice. The present study assessed the γ‐aminobutyric acid (GABA) and nitrogen (N) application induced regulations in the biochemical basis of rice aroma formation. Four N levels, that is, 0, 0.87, 1.75, and 2.61 g/pot, and two GABA treatments, that is, 0 mg/L (GABA0) and 250 mg/L (GABA250), were applied to three fragrant rice cultivars, that is, Yuxiangyouzhan, Yungengyou 14, and Basmati‐385. Results showed that GABA250 increased 2AP, Na, Mn, Zn, and Fe contents by 8.44%, 10.95%, 25.70%, 11.14%, and 43.30%, respectively, under N treatments across cultivars. The GABA250 further enhanced the activities of proline dehydrogenase (PDH), ornithine aminotransferase (OAT) (both at 15 days after heading (d AH), and diamine oxidase (DAO) (at maturity) by 20.36%, 11.24%, and 17.71%, respectively. Significant interaction between GABA and N for Mn, Zn, and Fe contents in grains, proline content in leaves, GABA content in leaves at 15 d AH and maturity stage (MS), Δ1‐pyrroline‐5‐carboxylic acid (P5C) contents in leaves at 15 d AH, and Δ1‐pyrroline‐5‐carboxylate synthase (P5CS), PDH, and OAT activities in leaves at MS was noted. Moreover, the 2AP contents in grains at MS showed a significant and positive correlation with the proline contents in the leaves at 15d AH. In conclusion, GABA250 enhanced the 2AP, Na, Mn, Zn, and Fe contents, as well as the enzyme activities involved in 2AP biosynthesis. Exogenous GABA and N application improved the 2AP contents and nutrient uptake in fragrant rice.

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Section: Introductionmentioning

confidence: 99%

Application of γ‐aminobutyric acid (GABA) and nitrogen regulates aroma biochemistry in fragrant rice

Xie

Ashraf

Zhong

et al. 2019

Food Science & Nutrition

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“…Our result for Navara calli size (30 days post-inoculation) is comparable with O. sativa L. cv. Khao Dawk Mali 105 (3 weeks old) calli size as 9.3 mm (Chutipaijit and Sutjaritvorakul 2018). The changes in fresh weight (mg) of the calli with time on the different CIM were noted.…”

Section: Discussionmentioning

confidence: 99%

Optimization of in vitro culture media for improvement in yield of Navara ancient Indian medicinal rice

2019

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Medicinally important ancient Navara rice (GI Kerala, India 2007) is a very short duration (60-70 days) variety with a yield of only 0.5 tonnes/hectare costing ~ Rs. 400/kg. It is used for indigenous treatment for chronic diseases by local and oral consumption. In this study, scutellum-derived calli were generated from mature Navara seeds and these were inoculated on different CIM-1 to CIM-5 media supplemented with 2.5 mg/l 2,4-dichlorophenoxyacetic acid. Regeneration of calli on different regeneration media RI, RII and RIII media were performed. Regeneration of 30-day-old calli on RI media showed 30%, for RII it showed no regeneration and on RIII media only 12% regeneration was obtained. The addition of glutamine and proline showed a 30-40% improvement in somatic embryogenesis. The 74-88% callus induction frequency was obtained on CIM-1 to CIM-5. The fresh weight (mg) of 30-day-old calli is CIM-2 < CIM-3 < CIM-4 < CIM-1 << CIM-5 and corresponding size shows CIM-2-CIM-3 < CIM-5 < CIM-1 < CIM-4. A negative correlation between the callus fresh weight and the regeneration efficiency was observed. In CIM-5, 20-25 days 3.4-fold increase and 25-30 days a 1.7-fold increase in fresh weight of calli is noted. The 20-day-old calli transfer to RI media shows 80% regeneration frequency and 6-7 plantlets/callus, which are twofold higher as compared with 30-day-old calli. The somatic embryogenesis and its regeneration on synthetic media provide an alternative for biotechnological intervention for yield improvement, in turn cost reduction for Navara rice.

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“…It can adsorb harmful substances present in culture media, including impurities in agar, 5-hydroxymethylfurfural produced by sucrose during high-pressure sterilization, and phenoquinones secreted by explants during culture, as well as beneficial substances available in culture media, such as growth regulators, vitamin B6, folic acid, and nicotinic acid [2]. There are many reports on its effects such as anti-browning, improvement of primary culture survival rates, promotion of bud proliferation and seedling growth in the dark, and promotion of rooting [3][4][5][6][7]. However, the mechanism of action of AC in promoting plant growth has been rarely reported.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture

Dong

Wang

et al. 2020

BMC Genet

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Background: Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth. Results: In total, we identified 18,555 differentially expressed genes (DEGs). Of these, 11,182 were detected in the roots and 7373 in the leaves. In seedlings grown in the presence of AC, 9460 DEGs were upregulated and 7483 DEGs were downregulated in the presence of AC as compared to the control. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 254 DEG-enriched pathways, 226 of which were common between roots and leaves. Further analysis of the major metabolic pathways revealed that AC stimulated the expression of nine genes in the phenylpropanoid biosynthesis pathway, including PLA, CYP73A, COMT, CYP84A, and 4CL, the protein products of which promote cell differentiation and seedling growth. Further, AC upregulated genes involved in plant hormone signaling related to stress resistance and disease resistance, including EIN3, BZR1, JAR1, JAZ, and PR1, and downregulated genes related to plant growth inhibition, including BKI1, ARR-B, DELLA, and ABF. Conclusions: Growth medium containing AC promotes seedling growth by increasing the expression of certain genes in the phenylpropanoid biosynthesis pathway, which are related to cell differentiation and seedling growth, as well as genes involved in plant hormone signaling, which is related to resistance.

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Application of activated charcoal and nanocarbon to callus induction and plant regeneration in aromatic rice (Oryza sativa L.)

Cited by 20 publications

References 56 publications

Application of γ‐aminobutyric acid (GABA) and nitrogen regulates aroma biochemistry in fragrant rice

Application of γ‐aminobutyric acid (GABA) and nitrogen regulates aroma biochemistry in fragrant rice

Optimization of in vitro culture media for improvement in yield of Navara ancient Indian medicinal rice

Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture

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