Abstract. Pulsed accelerators with high dose microorganisms are poorly understood. The objectives of our study effects of pulsed electron beams on microorganisms; 2) microorganisms interesting in the context of food safety accelerators: URT-1 and URT-0.5. Experiments on the dose distribution in the layer of 80 mg/cm URT-1 showed that the penetration of radiation at URT more suitable than URT-0.5.We investigated (Klebsiella, Citrobacter, Staphylococcus aureus, Salmonella) and fungal (Aspergillus) food spoil for Staphylococcus aureus, Salmonella, fungus Aspergillus reduced by 40-50%, 11 kGy is the dose at which radappertization). The inactivation of accelerator URT-0.5.