Application of a trityl-based radical probe for measuring superoxide

Rizzi, Cécile; Samouilov, Alexandre; Kutala, Vijay Kumar; Parinandi, Narasimham L.; Zweíer, Jay L.; Kuppusamy, Periannan

doi:10.1016/j.freeradbiomed.2003.09.014

Cited by 81 publications

(93 citation statements)

References 29 publications

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“…Due to its hydrophilicity and negative charge it does not penetrate cell membranes and is thus suitable for detection of extracellular superoxide. However, the probe is not absolutely specific for superoxide because it is also oxidized by agents such as peroxyl radicals [22]. …”

Section: Stable Free Radicalsmentioning

confidence: 99%

Use of spectroscopic probes for detection of reactive oxygen species

Bartosz

2006

Clinica Chimica Acta

268

176

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Section: Stable Free Radicalsmentioning

confidence: 99%

Use of spectroscopic probes for detection of reactive oxygen species

Bartosz

2006

Clinica Chimica Acta

268

176

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“…One of them is TAM that specifically reacts with superoxide with second order rate constant 3.1 £ 10 3 M 21 s 21 resulting in the loss of EPR signal intensity [16]. We used TAM to detect the formation of superoxide during oxidation of DOPA by PO.…”

Section: Resultsmentioning

confidence: 99%

“…Detection of superoxide radicals by spin traps DEPMPO (10 mM) [14,15], TAM (10 mM) [16] and CP-H (1 mM) [17] spin traps have been used to detect formation of superoxide radicals. EPR spectrometer settings for DEPMPO spin trap experiments were as following: modulation amplitude 2 G, microwave power 20 mW.…”

Section: Sources Of Superoxide Radicalsmentioning

confidence: 99%

See 1 more Smart Citation

Superoxide and hydrogen peroxide formation during enzymatic oxidation of DOPA by phenoloxidase

Komarov

Slepneva

Глупов

et al. 2005

Free Radical Research

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Generation of superoxide anion and hydrogen peroxide during enzymatic oxidation of 3-(3,4-dihydroxyphenyl)-DL-alanine (DOPA) has been studied. The ability of DOPA to react with O2*- has been revealed. EPR spectrum of DOPA-semiquinone formed upon oxidation of DOPA by O2*- was observed using spin stabilization technique of ortho-semiquinones by Zn2+ ions. Simultaneously, the oxidation of DOPA by O2*- was found to produce hydrogen peroxide (H2O2). The analysis of H2O2 formation upon oxidation of DOPA by O2*- using 1-hydroxy-3-carboxy-pyrrolidine (CP-H), and SOD as competitive reagents for superoxide provides consistent values of the rate constant for the reaction between DOPA and O2*- being equal to (3.4+/-0.6)x10(5) M(-1) s(-1). The formation of H2O2 during enzymatic oxidation of DOPA by phenoloxidase (PO) has been shown. The H2O2 production was found to be SOD-sensitive. The inhibition of H2O2 production by SOD was about 25% indicating that H2O2 is produced both from superoxide anion and via two-electron reduction of oxygen at the enzyme. The attempts to detect superoxide production during enzymatic oxidation of DOPA using a number of spin traps failed apparently due to high value of the rate constant for DOPA interaction with O2*-.

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“…Ascorbate, urate, and glutathione may reduce both the free rdaical and the spin trap radical adduct to ESR-silent species [2]. Rizzi et al [14] presented a new spin trap, TAM OX063, a triarylmethyl radical which traps superoxide anion without being influenced by bioreduction.…”

Section: Spin Trappingmentioning

confidence: 99%