Application of a Translational Profiling Approach for the Comparative Analysis of CNS Cell Types

Abstract

In the above article, Figure 2A is stated to summarize data from Figures 1A and 1B; however, we inadvertently displayed a plot of a different data set that was collected with a similar but slightly different experimental design. The data in Figures 1A and 1B are from an experiment in which one group of flies underwent mock conditioning and an independent group was conditioned with electric shock, whereas the data in Figure 2A were from an experiment in which the same population of flies sequentially underwent mock conditioning and actual conditioning.We provide here a corrected graph for Figure 2A plotting the data from Figure 1. The new plot does not affect the description of the results in the paper or the conclusions drawn. We apologize for any inconvenience caused by this error.

Alternative Splicing in the Mammalian Nervous System: Recent Insights into Mechanisms and Functional Roles

“…Furthermore, it may identify cell-specific AS regulators based on differences in expression levels. The translating ribosome affinity purification (TRAP) methodology, in which mice are genetically engineered to produce a fluorescently tagged ribosomal protein in specialized cell types of interest (Doyle et al, 2008;Heiman et al, 2014;, can additionally be used to determine the translation profile of individual cell types and detect cell-specific AS factors and protein isoforms. Moreover, the CRISPR/Cas9 technique can be used to delete neuron subtype-specific exons of interest and examine the functions of corresponding isoforms.…”

The Treasury of the Commons: Making Use of Public Gene Expression Resources to Better Characterize the Molecular Diversity of Inhibitory Interneurons in the Cerebellar Cortex

“…Many of the genes reported here were not identified in an earlier screen of cerebellar inhibitory interneurons using a translational profiling approach with a focus on genes enriched in transgene-tagged subsets of inhibitory interneurons [40]. We would like to stress that several of the subset-specific genes identified here, but that were not identified in the study by Doyle et al [40], including Cnr1 [41] and Slc6a5 (Glyt2) [42], are known to be expressed at the protein level in cerebellar inhibitory interneurons, so our observations on transcript expression would be fully expected, in general, to translate to the protein level. Thus, our approach is a necessary and powerful complement to other approaches.…”

LGR5/GPR49 is implicated in motor neuron specification in nervous system

“…We then explored the LGR5 expression pattern in CNS. By taking advantage of another dataset (GSE13379) of singe cell transcriptome in CNS (gene profiles derived from genetically defined cell populations in translating ribosome affinity purification (TRAP) transgenic mice) [15], we demonstrated that LGR5 is specifically highly expressed in a spectrum of neurons including Cholinergic neurons in spinal cord, motor neurons in brain stem, and neurons in Layer 5a and 6 in cortex (Fig. 1).…”

“…SC, spinal cord; BS, brain stem; Ctx, Cortex; CB, cerebellum; BF, basal forebrain; CS, corpus striatum; OG, oligodendrocyte; Ste/Bas neurons, Stellate and Basket neurons; Cho, cholinergic; Pnoc, prepronociceptin; CCK, cholecystokinin; Cort, cortistatin; Drd1, dopamine receptor D1. Relative mRNA levels were normalized with GCRMA and filtered to remove probesets with low signal and those identified as background [15]. and D).…”