Application of a Thin-Film Transistor Array for Cellular-Resolution Electrophysiology and Electrochemistry

Abstract

The constant development and improvement of microelectromechanical systems (MEMS) have been given a great opportunity to develop new reliable microsystems devices for biomedical research. This article presents a locally addressable 2-D arrayed indium-tin oxide microelectrode platform with integrated thin-film transistors (TFTs) for biological and chemical sensing. Microelectrode arrays (MEAs) using TFT have the advantage of being transparent with a high density of microelectrodes on a large surface. In vitro 2-D electrical measurements on 28 parallel-connected lines selected from a 22 500 MEA were successfully performed with heart cells for the first time. Observation of cell contraction was performed simultaneously with an inverted microscope. Voltammetry and amperometry measurements were also demonstrated using the transparent microelectrodes as working electrodes and an integrated Ag/AgCl reference electrode. Due to its unique features, we believe that the TFT platform can provide more understanding of the key communication between heart cells, and large-scale cardiovascular and nervous systems. As a result, the platform offers unique access to versatile Manuscript

Multi-modal Thin-Film-Transistor Biosensing Platforms for Bio-medical Investigations: Contribution to Internet-of-Medical-Things

“…Finally, the authors demonstrated cyclic voltammetry and amperometry experimental results with the TFT-device, in agreement with results obtained with a standard gold working electrode. Amperometry was performed on Tyramine and the smallest concentration detected was 10μM [7].…”

34‐3: Sensing and Biomimetic Stimulation of Cardiomyocyte Cell Culture with a Thin‐Film‐Transistor Active‐Matrix Platform

“…1). Our group has already published results demonstrating measurement of the electrophysiology activity of cardiac muscle cells (cardiomyocyte cell) cultured on a TFT platform, as well as cell culture monitoring using impedance spectroscopy [6,7]. In this article, we present results of electrical stimulation of a cardiomyocyte cell culture with a TFT platform.…”

Toward the development of a label-free multiple immunosensor based on thin film transistor microelectrode arrays

“…Recently, the research group of the authors has got interest in using the TFT technology for biological applications, such as electrophysiology, cells culture monitoring by impedance sensing and bio-sensing [3][4][5]. Actually, when compared to commercial MEAs with 64 electrodes per chip in general, TFT-µEAs are advantageous in higher electrode density with about 1.2 × 10 4 electrodes cm -2 (50-100 µm pitches and ∼5 µm spacing between neighboring electrodes) to avoid misaligning with cells, size of which is about 10-20 µm in diameter [6,7].…”

“…Moreover, Bakkum et al have conducted a two-dimensional mapping analysis for cell viability and function using human-derived cells, such as human hepatocarcinoma cell line Hep G2, by electrical impedance variation simultaneously with microscopic observation of fluorescent imaging on the transparent TFT-µEAs [2]. Furthermore, real-time extracellular potential measurement for cardiomyocytes and neural cells was also performed with similar devices toward the application to electrophysiological research [4,5]. It offers an alternative to MEA and CMOS for characterizing two-dimensional analyses of cardiomyocytes and neural cells on a large surface, with using high resolution and a dimension of pixels is as small as few cells.…”