Application of a spectrofluorimetric tool (bbe BenthoTorch) for monitoring potentially toxic benthic cyanobacteria in rivers

Echenique‐Subiabre, Isidora; Dalle, Caroline; Duval, Clément; Heath, Mark W.; Couté, Alain; Wood, Susanna A.; Humbert, Jean‐François; Quiblier, Catherine

doi:10.1016/j.watres.2016.05.081

Cited by 48 publications

(38 citation statements)

References 36 publications

(41 reference statements)

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“…The BenthoTorch is a hand held instrument that analyses Chl‐ a pigments in‐situ by fluorescence. This instrument has been shown to compare well with conventional spectroscopic‐based methods for Chl‐ a analysis (Kahlert & McKie, ), especially when the biofilm is thin (Echenique‐Subiabre et al., ), as was the case in our study. The BenthoTorch measures an area of 1.1 cm 2 and we systematically measured three separate locations on each NDS surface to produce a representative mean Chl‐ a number for each surface.…”

Section: Methodssupporting

confidence: 67%

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Persistent nitrogen limitation of stream biofilm communities along climate gradients in the Arctic

Myrstener

Rocher-Ros

Burrows

et al. 2018

Global Change Biology

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Climate change is rapidly reshaping Arctic landscapes through shifts in vegetation cover and productivity, soil resource mobilization, and hydrological regimes. The implications of these changes for stream ecosystems and food webs is unclear and will depend largely on microbial biofilm responses to concurrent shifts in temperature, light, and resource supply from land. To study those responses, we used nutrient diffusing substrates to manipulate resource supply to biofilm communities along regional gradients in stream temperature, riparian shading, and dissolved organic carbon (DOC) loading in Arctic Sweden. We found strong nitrogen (N) limitation across this gradient for gross primary production, community respiration and chlorophyll-a accumulation. For unamended biofilms, activity and biomass accrual were not closely related to any single physical or chemical driver across this region. However, the magnitude of biofilm response to N addition was: in tundra streams, biofilm response was constrained by thermal regimes, whereas variation in light availability regulated this response in birch and coniferous forest streams. Furthermore, heterotrophic responses to experimental N addition increased across the region with greater stream water concentrations of DOC relative to inorganic N. Thus, future shifts in resource supply to these ecosystems are likely to interact with other concurrent environmental changes to regulate stream productivity. Indeed, our results suggest that in the absence of increased nutrient inputs, Arctic streams will be less sensitive to future changes in other habitat variables such as temperature and DOC loading.

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Section: Methodssupporting

confidence: 67%

“…BenthoTorch is a hand held instrument that analyses Chl-a pigments in-situ by fluorescence. This instrument has been shown to compare well with conventional spectroscopic-based methods for Chl-a analysis (Kahlert & McKie, 2014), especially when the biofilm is thin (Echenique-Subiabre et al, 2016), as was the case in our study. The…”

Section: Chl-a Analysissupporting

confidence: 69%

Persistent nitrogen limitation of stream biofilm communities along climate gradients in the Arctic

Myrstener

Rocher-Ros

Burrows

et al. 2018

Global Change Biology

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“…54 . The equation of Talling and Driver 55 was used to estimate the chlorophyll-a (Chl-a) concentration (µg L −1 ), which was then transformed to surface unit concentration (mg cm −2 ) using the filtered biomass volume value and the sampling area defined by the surface of an underwater viewer (707 cm 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…Identification and enumeration of the samples were performed as described previously 54 . Cells were only identified as belonging to cyanobacteria, diatoms or green algae.…”

Section: Methodsmentioning

confidence: 99%

Deciphering biodiversity and interactions between bacteria and microeukaryotes within epilithic biofilms from the Loue River, France

Zancarini

Echenique‐Subiabre

Debroas

et al. 2017

Sci Rep

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Epilithic river biofilms are complex matrix-enclosed communities harboring a great diversity of prokaryotic and eukaryotic microorganisms. Interactions between these communities and the relative impacts of environmental factors on their compositions are poorly understood. In this study, we assessed the spatio-temporal variation in the diversity and composition of bacterial and microeukaryotic communities within biofilms in a French river. Significant changes were found in the composition of these microbial communities over the sampling period and between the upstream and downstream stations. In addition, the beta diversity of the bacterial community tended to decrease along the river, mostly as a result of turnover. These changes could be caused by the different water temperatures and geological and hydrological river contexts at the sampling sites (from karst landscape to river plain). Finally, our network analysis showed multiple correlations among dominant OTUs. Among them, negative correlations between Rhodobacteraceae and two other dominant groups of photosynthetic microorganisms (cyanobacteria and diatoms) were particularly interesting, which raises the question of what environmental factors trigger the changes occurring in benthic microbial photosynthetic communities.

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“…Over the last decade there has been a marked increase in the distribution and intensity of benthic mat‐forming cyanobacteria in rivers and streams globally (Quiblier et al ). Some of these taxa also produce cyanotoxins (Quiblier et al ), which have been increasingly monitored in New Zealand (McAllister et al ), Europe (Echenique‐Subiabre et al ; Cantoral et al ), and the United States (e.g., California; California Water Boards ). Recently, Fetscher et al () reported that one‐third of the samples from streams in a large sample‐set from California contained one or more cyanotoxins of benthic origin.…”

Section: Introductionmentioning

confidence: 99%

Extracts from benthic anatoxin‐producing Phormidium are toxic to 3 macroinvertebrate taxa at environmentally relevant concentrations

Anderson

Voorhees

Phillips

et al. 2018

Enviro Toxic and Chemistry

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Toxin-producing cyanobacteria are increasing in rivers and streams globally, leading to growing concerns over their potential impacts on aquatic ecosystems. The present study was designed to culture field-collected Phormidium in the laboratory, identify individual species, conduct chemical analyses to identify cyanotoxins, and conduct toxicity tests to investigate the potential for this genera to impact stream health. Freshwater toxicity tests were conducted with standard US Environmental Protection Agency invertebrate test protocols with culture water used to grow 3 Phormidium strains isolated from the Russian River (CA, USA). Enzyme linked immunosorbent assays were used to measure total anatoxin concentrations. Culture waters from the 3 Phormidium strains were highly toxic to Ceriodaphnia dubia, Hyalella azteca, and Chironomus dilutus. The C. dubia 7-d survival median lethal concentrations were 0.71, 0.49, and 0.56 μg/L anatoxin for Phormidum strains 1, 2, and 3, respectively. The 7-d reproduction inhibitory concentrations, 25% were 0.55, 0.32, and 0.30 μg/L anatoxin for strains 1, 2, and 3, respectively. Chironomus dilutus survival was reduced at concentrations <2 μg/L anatoxin by all 3 strains, and the H. azteca 96-h lethal concentrations, 25% were 2.82, 1.26, and 5.30 μg/L for strains 1, 2, and 3, respectively. Additional liquid chromatography-mass spectrometry analyses demonstrated that the likely anatoxin variant in these cultures was dihydro-anatoxin-a. The results suggest that anatoxins produced by Phormidium have the potential to impact stream macroinvertebrates. Environ Toxicol Chem 2018;37:2851-2859. © 2018 SETAC.

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Application of a spectrofluorimetric tool (bbe BenthoTorch) for monitoring potentially toxic benthic cyanobacteria in rivers

Cited by 48 publications

References 36 publications

Persistent nitrogen limitation of stream biofilm communities along climate gradients in the Arctic

Persistent nitrogen limitation of stream biofilm communities along climate gradients in the Arctic

Deciphering biodiversity and interactions between bacteria and microeukaryotes within epilithic biofilms from the Loue River, France

Extracts from benthic anatoxin‐producing Phormidium are toxic to 3 macroinvertebrate taxa at environmentally relevant concentrations

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